Experiment: BHIS with Dimetridazole 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1739 and BT1740 are separated by 72 nucleotides BT1740 and BT1741 are separated by 4 nucleotides BT1741 and BT1742 overlap by 4 nucleotides
BT1739: BT1739 - excinuclease ABC subunit A (NCBI ptt file), at 2,144,331 to 2,147,102
BT1739
BT1740: BT1740 - hypothetical protein (NCBI ptt file), at 2,147,175 to 2,147,660
BT1740
BT1741: BT1741 - hypothetical protein (NCBI ptt file), at 2,147,665 to 2,147,895
BT1741
BT1742: BT1742 - carbon starvation protein A (NCBI ptt file), at 2,147,892 to 2,149,322
BT1742
Position (kb)
2147
2148 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2146.181 kb on - strand, within BT1739 at 2146.211 kb on + strand, within BT1739 at 2146.211 kb on + strand, within BT1739 at 2146.226 kb on + strand, within BT1739 at 2146.380 kb on + strand, within BT1739 at 2146.411 kb on + strand, within BT1739 at 2146.500 kb on - strand, within BT1739 at 2146.626 kb on + strand, within BT1739 at 2146.626 kb on + strand, within BT1739 at 2146.626 kb on + strand, within BT1739 at 2146.627 kb on - strand, within BT1739 at 2146.633 kb on + strand, within BT1739 at 2146.735 kb on - strand, within BT1739 at 2146.736 kb on + strand, within BT1739 at 2146.736 kb on + strand, within BT1739 at 2146.744 kb on + strand, within BT1739 at 2146.761 kb on - strand, within BT1739 at 2146.946 kb on + strand at 2146.947 kb on - strand at 2146.949 kb on + strand at 2146.949 kb on + strand at 2146.974 kb on + strand at 2146.979 kb on - strand at 2147.030 kb on - strand at 2147.067 kb on + strand at 2147.069 kb on + strand at 2147.069 kb on + strand at 2147.121 kb on + strand at 2147.122 kb on - strand at 2147.159 kb on + strand at 2147.182 kb on + strand at 2147.201 kb on + strand at 2147.245 kb on + strand, within BT1740 at 2147.246 kb on - strand, within BT1740 at 2147.246 kb on - strand, within BT1740 at 2147.248 kb on + strand, within BT1740 at 2147.250 kb on + strand, within BT1740 at 2147.402 kb on - strand, within BT1740 at 2147.402 kb on - strand, within BT1740 at 2147.421 kb on + strand, within BT1740 at 2147.429 kb on - strand, within BT1740 at 2147.498 kb on + strand, within BT1740 at 2147.543 kb on + strand, within BT1740 at 2147.546 kb on - strand, within BT1740 at 2147.616 kb on + strand at 2147.752 kb on + strand, within BT1741 at 2147.752 kb on + strand, within BT1741 at 2147.826 kb on - strand, within BT1741 at 2147.846 kb on + strand, within BT1741 at 2147.847 kb on - strand, within BT1741 at 2147.934 kb on - strand at 2147.948 kb on + strand at 2147.949 kb on - strand at 2147.949 kb on - strand at 2147.959 kb on - strand at 2147.989 kb on - strand at 2148.021 kb on - strand at 2148.021 kb on - strand at 2148.021 kb on - strand at 2148.024 kb on + strand at 2148.024 kb on + strand at 2148.025 kb on - strand at 2148.025 kb on - strand at 2148.025 kb on - strand at 2148.025 kb on - strand at 2148.026 kb on + strand at 2148.027 kb on - strand at 2148.027 kb on - strand at 2148.028 kb on + strand at 2148.029 kb on - strand at 2148.054 kb on + strand, within BT1742 at 2148.055 kb on - strand, within BT1742 at 2148.113 kb on + strand, within BT1742 at 2148.177 kb on - strand, within BT1742 at 2148.200 kb on + strand, within BT1742 at 2148.201 kb on - strand, within BT1742 at 2148.201 kb on - strand, within BT1742 at 2148.218 kb on + strand, within BT1742 at 2148.223 kb on - strand, within BT1742 at 2148.223 kb on - strand, within BT1742 at 2148.240 kb on - strand, within BT1742 at 2148.248 kb on - strand, within BT1742 at 2148.323 kb on + strand, within BT1742 at 2148.380 kb on + strand, within BT1742 at 2148.380 kb on + strand, within BT1742 at 2148.398 kb on + strand, within BT1742 at 2148.461 kb on - strand, within BT1742 at 2148.481 kb on - strand, within BT1742 at 2148.481 kb on - strand, within BT1742 at 2148.518 kb on - strand, within BT1742
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimetridazole 0.0125 mM remove 2,146,181 - BT1739 0.67 -4.7 2,146,211 + BT1739 0.68 -2.6 2,146,211 + BT1739 0.68 -3.7 2,146,226 + BT1739 0.68 -4.5 2,146,380 + BT1739 0.74 -1.8 2,146,411 + BT1739 0.75 -2.8 2,146,500 - BT1739 0.78 -0.0 2,146,626 + BT1739 0.83 -4.1 2,146,626 + BT1739 0.83 -3.2 2,146,626 + BT1739 0.83 -1.4 2,146,627 - BT1739 0.83 +1.0 2,146,633 + BT1739 0.83 -5.5 2,146,735 - BT1739 0.87 -5.4 2,146,736 + BT1739 0.87 -1.0 2,146,736 + BT1739 0.87 -3.4 2,146,744 + BT1739 0.87 -2.5 2,146,761 - BT1739 0.88 -4.4 2,146,946 + -0.6 2,146,947 - -2.2 2,146,949 + -3.7 2,146,949 + -5.3 2,146,974 + -2.4 2,146,979 - -0.0 2,147,030 - -5.2 2,147,067 + -2.0 2,147,069 + -0.6 2,147,069 + -1.7 2,147,121 + -0.6 2,147,122 - -0.4 2,147,159 + -0.7 2,147,182 + -0.0 2,147,201 + -0.0 2,147,245 + BT1740 0.14 +0.2 2,147,246 - BT1740 0.15 -1.8 2,147,246 - BT1740 0.15 -0.4 2,147,248 + BT1740 0.15 -1.8 2,147,250 + BT1740 0.15 -0.6 2,147,402 - BT1740 0.47 -0.6 2,147,402 - BT1740 0.47 -0.0 2,147,421 + BT1740 0.51 -0.0 2,147,429 - BT1740 0.52 -0.0 2,147,498 + BT1740 0.66 -0.3 2,147,543 + BT1740 0.76 -0.1 2,147,546 - BT1740 0.76 +1.0 2,147,616 + -0.7 2,147,752 + BT1741 0.38 -0.6 2,147,752 + BT1741 0.38 -0.6 2,147,826 - BT1741 0.70 -0.9 2,147,846 + BT1741 0.78 +0.4 2,147,847 - BT1741 0.79 -0.6 2,147,934 - +1.0 2,147,948 + -1.5 2,147,949 - -0.6 2,147,949 - -1.1 2,147,959 - -1.0 2,147,989 - -1.3 2,148,021 - +0.1 2,148,021 - -0.0 2,148,021 - -1.4 2,148,024 + -0.6 2,148,024 + +0.2 2,148,025 - -1.2 2,148,025 - -1.0 2,148,025 - -0.1 2,148,025 - -0.5 2,148,026 + -1.7 2,148,027 - -0.0 2,148,027 - -0.3 2,148,028 + -0.0 2,148,029 - -0.6 2,148,054 + BT1742 0.11 +1.4 2,148,055 - BT1742 0.11 -0.0 2,148,113 + BT1742 0.15 -2.4 2,148,177 - BT1742 0.20 -1.0 2,148,200 + BT1742 0.22 -0.5 2,148,201 - BT1742 0.22 +1.0 2,148,201 - BT1742 0.22 +1.0 2,148,218 + BT1742 0.23 -0.0 2,148,223 - BT1742 0.23 -0.6 2,148,223 - BT1742 0.23 -1.8 2,148,240 - BT1742 0.24 -2.5 2,148,248 - BT1742 0.25 -0.0 2,148,323 + BT1742 0.30 +0.2 2,148,380 + BT1742 0.34 +1.0 2,148,380 + BT1742 0.34 -0.8 2,148,398 + BT1742 0.35 -1.0 2,148,461 - BT1742 0.40 -0.1 2,148,481 - BT1742 0.41 -1.8 2,148,481 - BT1742 0.41 +0.4 2,148,518 - BT1742 0.44 +1.0
Or see this region's nucleotide sequence