Experiment: BHIS with Dimetridazole 0.0125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1359 and BT1360 are separated by 1 nucleotides BT1360 and BT1361 are separated by 33 nucleotides
BT1359: BT1359 - Tetratricopeptide repeat family protein (NCBI ptt file), at 1,685,751 to 1,687,457
BT1359
BT1360: BT1360 - tRNA/rRNA methyltransferase (NCBI ptt file), at 1,687,459 to 1,688,202
BT1360
BT1361: BT1361 - DNA repair protein recN (Recombination protein N) (NCBI ptt file), at 1,688,236 to 1,689,903
BT1361
Position (kb)
1687
1688
1689 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1686.470 kb on - strand, within BT1359 at 1686.518 kb on - strand, within BT1359 at 1686.518 kb on - strand, within BT1359 at 1686.518 kb on - strand, within BT1359 at 1686.537 kb on - strand, within BT1359 at 1686.592 kb on - strand, within BT1359 at 1686.633 kb on + strand, within BT1359 at 1686.640 kb on + strand, within BT1359 at 1686.641 kb on - strand, within BT1359 at 1686.658 kb on + strand, within BT1359 at 1686.659 kb on - strand, within BT1359 at 1686.659 kb on - strand, within BT1359 at 1686.752 kb on - strand, within BT1359 at 1686.918 kb on - strand, within BT1359 at 1686.990 kb on - strand, within BT1359 at 1686.991 kb on + strand, within BT1359 at 1686.991 kb on + strand, within BT1359 at 1686.992 kb on - strand, within BT1359 at 1686.995 kb on - strand, within BT1359 at 1686.995 kb on - strand, within BT1359 at 1687.001 kb on - strand, within BT1359 at 1687.064 kb on + strand, within BT1359 at 1687.070 kb on - strand, within BT1359 at 1687.348 kb on - strand at 1687.443 kb on + strand at 1687.457 kb on + strand at 1688.238 kb on - strand at 1688.386 kb on - strand at 1688.386 kb on - strand at 1688.388 kb on - strand at 1688.389 kb on + strand at 1688.501 kb on - strand, within BT1361 at 1688.627 kb on - strand, within BT1361 at 1688.690 kb on - strand, within BT1361 at 1688.889 kb on - strand, within BT1361 at 1688.889 kb on - strand, within BT1361 at 1688.889 kb on - strand, within BT1361 at 1688.889 kb on - strand, within BT1361
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimetridazole 0.0125 mM remove 1,686,470 - BT1359 0.42 +0.8 1,686,518 - BT1359 0.45 +0.1 1,686,518 - BT1359 0.45 +0.4 1,686,518 - BT1359 0.45 -0.4 1,686,537 - BT1359 0.46 -1.6 1,686,592 - BT1359 0.49 +1.0 1,686,633 + BT1359 0.52 -0.6 1,686,640 + BT1359 0.52 -1.7 1,686,641 - BT1359 0.52 -0.0 1,686,658 + BT1359 0.53 +0.4 1,686,659 - BT1359 0.53 +1.0 1,686,659 - BT1359 0.53 -0.0 1,686,752 - BT1359 0.59 -0.0 1,686,918 - BT1359 0.68 +1.0 1,686,990 - BT1359 0.73 -0.0 1,686,991 + BT1359 0.73 -0.6 1,686,991 + BT1359 0.73 -0.0 1,686,992 - BT1359 0.73 -1.3 1,686,995 - BT1359 0.73 -0.8 1,686,995 - BT1359 0.73 -0.0 1,687,001 - BT1359 0.73 -1.2 1,687,064 + BT1359 0.77 -1.3 1,687,070 - BT1359 0.77 +1.0 1,687,348 - +2.0 1,687,443 + +1.0 1,687,457 + -1.0 1,688,238 - -2.1 1,688,386 - -0.8 1,688,386 - -0.6 1,688,388 - -1.4 1,688,389 + -1.3 1,688,501 - BT1361 0.16 -3.1 1,688,627 - BT1361 0.23 -1.3 1,688,690 - BT1361 0.27 -1.6 1,688,889 - BT1361 0.39 -3.7 1,688,889 - BT1361 0.39 -3.2 1,688,889 - BT1361 0.39 -0.6 1,688,889 - BT1361 0.39 -4.0
Or see this region's nucleotide sequence