Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20910

Experiment: L-Isoleucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20905 and BWI76_RS20910 are separated by 153 nucleotidesBWI76_RS20910 and BWI76_RS20915 are separated by 3 nucleotidesBWI76_RS20915 and BWI76_RS20920 are separated by 78 nucleotides BWI76_RS20905: BWI76_RS20905 - 4-hydroxy-tetrahydrodipicolinate synthase, at 4,371,468 to 4,372,346 _RS20905 BWI76_RS20910: BWI76_RS20910 - glycine cleavage system transcriptional repressor, at 4,372,500 to 4,373,069 _RS20910 BWI76_RS20915: BWI76_RS20915 - peroxiredoxin, at 4,373,073 to 4,373,543 _RS20915 BWI76_RS20920: BWI76_RS20920 - AI-2E family transporter, at 4,373,622 to 4,374,689 _RS20920 Position (kb) 4372 4373 4374Strain fitness (log2 ratio) -2 -1 0 1at 4372.500 kb on - strandat 4372.526 kb on + strandat 4372.537 kb on + strandat 4372.538 kb on + strandat 4372.686 kb on - strand, within BWI76_RS20910at 4372.701 kb on - strand, within BWI76_RS20910at 4372.706 kb on + strandat 4372.951 kb on - strand, within BWI76_RS20910at 4372.991 kb on + strand, within BWI76_RS20910at 4372.997 kb on - strand, within BWI76_RS20910at 4373.057 kb on + strandat 4373.106 kb on + strandat 4373.204 kb on + strand, within BWI76_RS20915at 4373.212 kb on + strand, within BWI76_RS20915at 4373.212 kb on + strand, within BWI76_RS20915at 4373.217 kb on - strand, within BWI76_RS20915at 4373.219 kb on + strand, within BWI76_RS20915at 4373.220 kb on - strand, within BWI76_RS20915at 4373.222 kb on - strand, within BWI76_RS20915at 4373.417 kb on - strand, within BWI76_RS20915at 4373.514 kb on + strandat 4373.790 kb on - strand, within BWI76_RS20920at 4373.883 kb on - strand, within BWI76_RS20920at 4373.969 kb on + strand, within BWI76_RS20920at 4374.001 kb on - strand, within BWI76_RS20920

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (N)
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4,372,500 - +1.4
4,372,526 + +0.3
4,372,537 + +1.6
4,372,538 + +1.6
4,372,686 - BWI76_RS20910 0.33 +0.2
4,372,701 - BWI76_RS20910 0.35 +1.4
4,372,706 + -0.1
4,372,951 - BWI76_RS20910 0.79 +1.4
4,372,991 + BWI76_RS20910 0.86 +1.5
4,372,997 - BWI76_RS20910 0.87 +1.4
4,373,057 + +1.5
4,373,106 + +0.4
4,373,204 + BWI76_RS20915 0.28 +0.1
4,373,212 + BWI76_RS20915 0.30 -0.7
4,373,212 + BWI76_RS20915 0.30 -0.0
4,373,217 - BWI76_RS20915 0.31 -0.1
4,373,219 + BWI76_RS20915 0.31 -0.0
4,373,220 - BWI76_RS20915 0.31 -0.4
4,373,222 - BWI76_RS20915 0.32 -0.3
4,373,417 - BWI76_RS20915 0.73 -1.9
4,373,514 + -0.1
4,373,790 - BWI76_RS20920 0.16 -0.0
4,373,883 - BWI76_RS20920 0.24 +0.3
4,373,969 + BWI76_RS20920 0.32 +0.2
4,374,001 - BWI76_RS20920 0.35 +0.2

Or see this region's nucleotide sequence