Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20905 and BWI76_RS20910 are separated by 153 nucleotides BWI76_RS20910 and BWI76_RS20915 are separated by 3 nucleotides BWI76_RS20915 and BWI76_RS20920 are separated by 78 nucleotides
BWI76_RS20905: BWI76_RS20905 - 4-hydroxy-tetrahydrodipicolinate synthase, at 4,371,468 to 4,372,346
_RS20905
BWI76_RS20910: BWI76_RS20910 - glycine cleavage system transcriptional repressor, at 4,372,500 to 4,373,069
_RS20910
BWI76_RS20915: BWI76_RS20915 - peroxiredoxin, at 4,373,073 to 4,373,543
_RS20915
BWI76_RS20920: BWI76_RS20920 - AI-2E family transporter, at 4,373,622 to 4,374,689
_RS20920
Position (kb)
4372
4373
4374 Strain fitness (log2 ratio)
-2
-1
0
1 at 4372.500 kb on - strand at 4372.526 kb on + strand at 4372.537 kb on + strand at 4372.538 kb on + strand at 4372.686 kb on - strand, within BWI76_RS20910 at 4372.701 kb on - strand, within BWI76_RS20910 at 4372.706 kb on + strand at 4372.951 kb on - strand, within BWI76_RS20910 at 4372.991 kb on + strand, within BWI76_RS20910 at 4372.997 kb on - strand, within BWI76_RS20910 at 4373.057 kb on + strand at 4373.106 kb on + strand at 4373.204 kb on + strand, within BWI76_RS20915 at 4373.212 kb on + strand, within BWI76_RS20915 at 4373.212 kb on + strand, within BWI76_RS20915 at 4373.217 kb on - strand, within BWI76_RS20915 at 4373.219 kb on + strand, within BWI76_RS20915 at 4373.220 kb on - strand, within BWI76_RS20915 at 4373.222 kb on - strand, within BWI76_RS20915 at 4373.417 kb on - strand, within BWI76_RS20915 at 4373.514 kb on + strand at 4373.790 kb on - strand, within BWI76_RS20920 at 4373.883 kb on - strand, within BWI76_RS20920 at 4373.969 kb on + strand, within BWI76_RS20920 at 4374.001 kb on - strand, within BWI76_RS20920
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 4,372,500 - +1.4 4,372,526 + +0.3 4,372,537 + +1.6 4,372,538 + +1.6 4,372,686 - BWI76_RS20910 0.33 +0.2 4,372,701 - BWI76_RS20910 0.35 +1.4 4,372,706 + -0.1 4,372,951 - BWI76_RS20910 0.79 +1.4 4,372,991 + BWI76_RS20910 0.86 +1.5 4,372,997 - BWI76_RS20910 0.87 +1.4 4,373,057 + +1.5 4,373,106 + +0.4 4,373,204 + BWI76_RS20915 0.28 +0.1 4,373,212 + BWI76_RS20915 0.30 -0.7 4,373,212 + BWI76_RS20915 0.30 -0.0 4,373,217 - BWI76_RS20915 0.31 -0.1 4,373,219 + BWI76_RS20915 0.31 -0.0 4,373,220 - BWI76_RS20915 0.31 -0.4 4,373,222 - BWI76_RS20915 0.32 -0.3 4,373,417 - BWI76_RS20915 0.73 -1.9 4,373,514 + -0.1 4,373,790 - BWI76_RS20920 0.16 -0.0 4,373,883 - BWI76_RS20920 0.24 +0.3 4,373,969 + BWI76_RS20920 0.32 +0.2 4,374,001 - BWI76_RS20920 0.35 +0.2
Or see this region's nucleotide sequence