Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17055 and BWI76_RS17065 are separated by 400 nucleotides BWI76_RS17065 and BWI76_RS17070 are separated by 342 nucleotides
BWI76_RS17055: BWI76_RS17055 - hypothetical protein, at 3,538,538 to 3,539,659
_RS17055
BWI76_RS17065: BWI76_RS17065 - phosphoenolpyruvate synthase, at 3,540,060 to 3,542,438
_RS17065
BWI76_RS17070: BWI76_RS17070 - phosphoenolpyruvate synthase regulatory protein, at 3,542,781 to 3,543,614
_RS17070
Position (kb)
3540
3541
3542
3543 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3539.214 kb on - strand, within BWI76_RS17055 at 3539.214 kb on - strand, within BWI76_RS17055 at 3539.232 kb on + strand, within BWI76_RS17055 at 3539.240 kb on - strand, within BWI76_RS17055 at 3539.325 kb on - strand, within BWI76_RS17055 at 3539.439 kb on - strand, within BWI76_RS17055 at 3539.555 kb on - strand at 3539.569 kb on + strand at 3539.706 kb on - strand at 3539.940 kb on + strand at 3539.940 kb on + strand at 3540.597 kb on - strand, within BWI76_RS17065 at 3540.799 kb on + strand, within BWI76_RS17065 at 3540.800 kb on - strand, within BWI76_RS17065 at 3540.880 kb on + strand, within BWI76_RS17065 at 3541.317 kb on + strand, within BWI76_RS17065 at 3541.393 kb on - strand, within BWI76_RS17065 at 3541.393 kb on - strand, within BWI76_RS17065 at 3541.393 kb on - strand, within BWI76_RS17065 at 3541.393 kb on - strand, within BWI76_RS17065 at 3541.444 kb on + strand, within BWI76_RS17065 at 3541.452 kb on - strand, within BWI76_RS17065 at 3541.617 kb on + strand, within BWI76_RS17065 at 3541.630 kb on - strand, within BWI76_RS17065 at 3542.225 kb on + strand at 3542.322 kb on + strand at 3542.363 kb on + strand at 3542.368 kb on + strand at 3542.381 kb on - strand at 3542.381 kb on - strand at 3542.381 kb on - strand at 3542.381 kb on - strand at 3542.391 kb on + strand at 3542.396 kb on + strand at 3542.396 kb on + strand at 3542.404 kb on - strand at 3542.404 kb on - strand at 3542.411 kb on - strand at 3542.428 kb on + strand at 3542.430 kb on + strand at 3542.430 kb on + strand at 3542.444 kb on - strand at 3542.496 kb on + strand at 3542.510 kb on + strand at 3542.581 kb on + strand at 3542.600 kb on - strand at 3542.761 kb on + strand at 3542.788 kb on + strand at 3542.788 kb on + strand at 3542.788 kb on + strand at 3542.788 kb on + strand at 3542.796 kb on - strand at 3542.796 kb on - strand at 3542.844 kb on + strand at 3542.844 kb on + strand at 3542.844 kb on + strand at 3542.877 kb on + strand, within BWI76_RS17070 at 3542.885 kb on + strand, within BWI76_RS17070 at 3542.890 kb on + strand, within BWI76_RS17070 at 3542.940 kb on + strand, within BWI76_RS17070 at 3543.189 kb on - strand, within BWI76_RS17070 at 3543.220 kb on + strand, within BWI76_RS17070
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,539,214 - BWI76_RS17055 0.60 -0.3 3,539,214 - BWI76_RS17055 0.60 +0.4 3,539,232 + BWI76_RS17055 0.62 -0.1 3,539,240 - BWI76_RS17055 0.63 +1.1 3,539,325 - BWI76_RS17055 0.70 -0.1 3,539,439 - BWI76_RS17055 0.80 -0.9 3,539,555 - -1.1 3,539,569 + -0.8 3,539,706 - -0.8 3,539,940 + +0.7 3,539,940 + -1.2 3,540,597 - BWI76_RS17065 0.23 -0.1 3,540,799 + BWI76_RS17065 0.31 -0.1 3,540,800 - BWI76_RS17065 0.31 +0.2 3,540,880 + BWI76_RS17065 0.34 -0.5 3,541,317 + BWI76_RS17065 0.53 +0.9 3,541,393 - BWI76_RS17065 0.56 +0.4 3,541,393 - BWI76_RS17065 0.56 -1.4 3,541,393 - BWI76_RS17065 0.56 +0.5 3,541,393 - BWI76_RS17065 0.56 +0.0 3,541,444 + BWI76_RS17065 0.58 +0.3 3,541,452 - BWI76_RS17065 0.59 +0.4 3,541,617 + BWI76_RS17065 0.65 -0.1 3,541,630 - BWI76_RS17065 0.66 -0.2 3,542,225 + -1.8 3,542,322 + +0.1 3,542,363 + +0.2 3,542,368 + +0.3 3,542,381 - +0.1 3,542,381 - -0.7 3,542,381 - -0.8 3,542,381 - +0.1 3,542,391 + -3.4 3,542,396 + -0.9 3,542,396 + -0.6 3,542,404 - -2.5 3,542,404 - -0.3 3,542,411 - +0.5 3,542,428 + +0.8 3,542,430 + +1.0 3,542,430 + -0.9 3,542,444 - -0.3 3,542,496 + -0.3 3,542,510 + -0.9 3,542,581 + -0.9 3,542,600 - -0.4 3,542,761 + +0.6 3,542,788 + +0.3 3,542,788 + +0.7 3,542,788 + -1.3 3,542,788 + -0.3 3,542,796 - +0.5 3,542,796 - -1.6 3,542,844 + -1.1 3,542,844 + +0.0 3,542,844 + -0.3 3,542,877 + BWI76_RS17070 0.12 +0.7 3,542,885 + BWI76_RS17070 0.12 -0.6 3,542,890 + BWI76_RS17070 0.13 -0.2 3,542,940 + BWI76_RS17070 0.19 -0.5 3,543,189 - BWI76_RS17070 0.49 -0.4 3,543,220 + BWI76_RS17070 0.53 +1.2
Or see this region's nucleotide sequence