Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS17065

Experiment: L-Isoleucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS17055 and BWI76_RS17065 are separated by 400 nucleotidesBWI76_RS17065 and BWI76_RS17070 are separated by 342 nucleotides BWI76_RS17055: BWI76_RS17055 - hypothetical protein, at 3,538,538 to 3,539,659 _RS17055 BWI76_RS17065: BWI76_RS17065 - phosphoenolpyruvate synthase, at 3,540,060 to 3,542,438 _RS17065 BWI76_RS17070: BWI76_RS17070 - phosphoenolpyruvate synthase regulatory protein, at 3,542,781 to 3,543,614 _RS17070 Position (kb) 3540 3541 3542 3543Strain fitness (log2 ratio) -3 -2 -1 0 1at 3539.214 kb on - strand, within BWI76_RS17055at 3539.214 kb on - strand, within BWI76_RS17055at 3539.232 kb on + strand, within BWI76_RS17055at 3539.240 kb on - strand, within BWI76_RS17055at 3539.325 kb on - strand, within BWI76_RS17055at 3539.439 kb on - strand, within BWI76_RS17055at 3539.555 kb on - strandat 3539.569 kb on + strandat 3539.706 kb on - strandat 3539.940 kb on + strandat 3539.940 kb on + strandat 3540.597 kb on - strand, within BWI76_RS17065at 3540.799 kb on + strand, within BWI76_RS17065at 3540.800 kb on - strand, within BWI76_RS17065at 3540.880 kb on + strand, within BWI76_RS17065at 3541.317 kb on + strand, within BWI76_RS17065at 3541.393 kb on - strand, within BWI76_RS17065at 3541.393 kb on - strand, within BWI76_RS17065at 3541.393 kb on - strand, within BWI76_RS17065at 3541.393 kb on - strand, within BWI76_RS17065at 3541.444 kb on + strand, within BWI76_RS17065at 3541.452 kb on - strand, within BWI76_RS17065at 3541.617 kb on + strand, within BWI76_RS17065at 3541.630 kb on - strand, within BWI76_RS17065at 3542.225 kb on + strandat 3542.322 kb on + strandat 3542.363 kb on + strandat 3542.368 kb on + strandat 3542.381 kb on - strandat 3542.381 kb on - strandat 3542.381 kb on - strandat 3542.381 kb on - strandat 3542.391 kb on + strandat 3542.396 kb on + strandat 3542.396 kb on + strandat 3542.404 kb on - strandat 3542.404 kb on - strandat 3542.411 kb on - strandat 3542.428 kb on + strandat 3542.430 kb on + strandat 3542.430 kb on + strandat 3542.444 kb on - strandat 3542.496 kb on + strandat 3542.510 kb on + strandat 3542.581 kb on + strandat 3542.600 kb on - strandat 3542.761 kb on + strandat 3542.788 kb on + strandat 3542.788 kb on + strandat 3542.788 kb on + strandat 3542.788 kb on + strandat 3542.796 kb on - strandat 3542.796 kb on - strandat 3542.844 kb on + strandat 3542.844 kb on + strandat 3542.844 kb on + strandat 3542.877 kb on + strand, within BWI76_RS17070at 3542.885 kb on + strand, within BWI76_RS17070at 3542.890 kb on + strand, within BWI76_RS17070at 3542.940 kb on + strand, within BWI76_RS17070at 3543.189 kb on - strand, within BWI76_RS17070at 3543.220 kb on + strand, within BWI76_RS17070

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (N)
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3,539,214 - BWI76_RS17055 0.60 -0.3
3,539,214 - BWI76_RS17055 0.60 +0.4
3,539,232 + BWI76_RS17055 0.62 -0.1
3,539,240 - BWI76_RS17055 0.63 +1.1
3,539,325 - BWI76_RS17055 0.70 -0.1
3,539,439 - BWI76_RS17055 0.80 -0.9
3,539,555 - -1.1
3,539,569 + -0.8
3,539,706 - -0.8
3,539,940 + +0.7
3,539,940 + -1.2
3,540,597 - BWI76_RS17065 0.23 -0.1
3,540,799 + BWI76_RS17065 0.31 -0.1
3,540,800 - BWI76_RS17065 0.31 +0.2
3,540,880 + BWI76_RS17065 0.34 -0.5
3,541,317 + BWI76_RS17065 0.53 +0.9
3,541,393 - BWI76_RS17065 0.56 +0.4
3,541,393 - BWI76_RS17065 0.56 -1.4
3,541,393 - BWI76_RS17065 0.56 +0.5
3,541,393 - BWI76_RS17065 0.56 +0.0
3,541,444 + BWI76_RS17065 0.58 +0.3
3,541,452 - BWI76_RS17065 0.59 +0.4
3,541,617 + BWI76_RS17065 0.65 -0.1
3,541,630 - BWI76_RS17065 0.66 -0.2
3,542,225 + -1.8
3,542,322 + +0.1
3,542,363 + +0.2
3,542,368 + +0.3
3,542,381 - +0.1
3,542,381 - -0.7
3,542,381 - -0.8
3,542,381 - +0.1
3,542,391 + -3.4
3,542,396 + -0.9
3,542,396 + -0.6
3,542,404 - -2.5
3,542,404 - -0.3
3,542,411 - +0.5
3,542,428 + +0.8
3,542,430 + +1.0
3,542,430 + -0.9
3,542,444 - -0.3
3,542,496 + -0.3
3,542,510 + -0.9
3,542,581 + -0.9
3,542,600 - -0.4
3,542,761 + +0.6
3,542,788 + +0.3
3,542,788 + +0.7
3,542,788 + -1.3
3,542,788 + -0.3
3,542,796 - +0.5
3,542,796 - -1.6
3,542,844 + -1.1
3,542,844 + +0.0
3,542,844 + -0.3
3,542,877 + BWI76_RS17070 0.12 +0.7
3,542,885 + BWI76_RS17070 0.12 -0.6
3,542,890 + BWI76_RS17070 0.13 -0.2
3,542,940 + BWI76_RS17070 0.19 -0.5
3,543,189 - BWI76_RS17070 0.49 -0.4
3,543,220 + BWI76_RS17070 0.53 +1.2

Or see this region's nucleotide sequence