Experiment: Chloride 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_97 and Pf6N2E2_98 overlap by 4 nucleotides Pf6N2E2_98 and Pf6N2E2_99 are separated by 53 nucleotides Pf6N2E2_99 and Pf6N2E2_100 are separated by 298 nucleotides Pf6N2E2_100 and Pf6N2E2_101 are separated by 128 nucleotides Pf6N2E2_101 and Pf6N2E2_102 are separated by 338 nucleotides
Pf6N2E2_97 - putataive branched-chain amino acid transport protein AzlC, at 142,645 to 143,355
_97
Pf6N2E2_98 - putative transmembrane protein, at 143,352 to 143,660
_98
Pf6N2E2_99 - hypothetical protein, at 143,714 to 143,854
_99
Pf6N2E2_100 - membrane protein, putative, at 144,153 to 145,364
_100
Pf6N2E2_101 - CigR (Putative inner membrane protein), at 145,493 to 146,005
_101
Pf6N2E2_102 - sigma-54 dependent transcriptional regulator, at 146,344 to 148,188
_102
Position (kb) 144
145
146 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 143.230 kb on - strand, within Pf6N2E2_97 at 143.392 kb on + strand, within Pf6N2E2_98 at 143.393 kb on - strand, within Pf6N2E2_98 at 143.629 kb on + strand, within Pf6N2E2_98 at 143.630 kb on - strand at 143.757 kb on - strand, within Pf6N2E2_99 at 143.757 kb on - strand, within Pf6N2E2_99 at 143.757 kb on - strand, within Pf6N2E2_99 at 143.768 kb on - strand, within Pf6N2E2_99 at 143.870 kb on + strand at 143.923 kb on + strand at 143.923 kb on + strand at 143.923 kb on + strand at 143.923 kb on + strand at 143.923 kb on + strand at 143.923 kb on + strand at 143.924 kb on - strand at 143.924 kb on - strand at 143.954 kb on - strand at 143.954 kb on - strand at 143.976 kb on - strand at 144.117 kb on + strand at 144.117 kb on + strand at 144.118 kb on - strand at 144.118 kb on - strand at 144.118 kb on - strand at 144.118 kb on - strand at 144.128 kb on + strand at 144.129 kb on - strand at 144.129 kb on - strand at 144.129 kb on - strand at 144.129 kb on - strand at 144.132 kb on - strand at 144.132 kb on - strand at 144.132 kb on - strand at 144.132 kb on - strand at 144.132 kb on - strand at 144.172 kb on + strand at 144.172 kb on + strand at 144.172 kb on + strand at 144.173 kb on - strand at 144.173 kb on - strand at 144.196 kb on + strand at 144.196 kb on + strand at 144.196 kb on + strand at 144.197 kb on - strand at 144.197 kb on - strand at 144.197 kb on - strand at 144.276 kb on - strand, within Pf6N2E2_100 at 144.300 kb on + strand, within Pf6N2E2_100 at 144.403 kb on + strand, within Pf6N2E2_100 at 144.404 kb on - strand, within Pf6N2E2_100 at 144.511 kb on + strand, within Pf6N2E2_100 at 144.511 kb on + strand, within Pf6N2E2_100 at 144.512 kb on - strand, within Pf6N2E2_100 at 144.512 kb on - strand, within Pf6N2E2_100 at 144.600 kb on + strand, within Pf6N2E2_100 at 144.610 kb on + strand, within Pf6N2E2_100 at 144.610 kb on + strand, within Pf6N2E2_100 at 144.610 kb on + strand, within Pf6N2E2_100 at 144.631 kb on + strand, within Pf6N2E2_100 at 144.827 kb on - strand, within Pf6N2E2_100 at 144.900 kb on + strand, within Pf6N2E2_100 at 144.901 kb on - strand, within Pf6N2E2_100 at 144.951 kb on + strand, within Pf6N2E2_100 at 144.951 kb on + strand, within Pf6N2E2_100 at 144.961 kb on + strand, within Pf6N2E2_100 at 144.979 kb on + strand, within Pf6N2E2_100 at 144.980 kb on - strand, within Pf6N2E2_100 at 144.980 kb on - strand, within Pf6N2E2_100 at 144.980 kb on - strand, within Pf6N2E2_100 at 144.985 kb on + strand, within Pf6N2E2_100 at 144.985 kb on + strand, within Pf6N2E2_100 at 144.986 kb on - strand, within Pf6N2E2_100 at 144.986 kb on - strand, within Pf6N2E2_100 at 144.986 kb on - strand, within Pf6N2E2_100 at 144.986 kb on - strand, within Pf6N2E2_100 at 144.986 kb on - strand, within Pf6N2E2_100 at 145.000 kb on + strand, within Pf6N2E2_100 at 145.001 kb on - strand, within Pf6N2E2_100 at 145.079 kb on - strand, within Pf6N2E2_100 at 145.079 kb on - strand, within Pf6N2E2_100 at 145.136 kb on - strand, within Pf6N2E2_100 at 145.352 kb on - strand at 145.352 kb on - strand at 145.391 kb on + strand at 145.391 kb on + strand at 145.391 kb on + strand at 145.391 kb on + strand at 145.391 kb on + strand at 145.392 kb on - strand at 145.392 kb on - strand at 145.392 kb on - strand at 145.392 kb on - strand at 145.393 kb on + strand at 145.393 kb on + strand at 145.433 kb on + strand at 145.434 kb on - strand at 145.434 kb on - strand at 145.436 kb on + strand at 145.437 kb on - strand at 145.437 kb on - strand at 145.452 kb on + strand at 145.452 kb on + strand at 145.452 kb on + strand at 145.452 kb on + strand at 145.452 kb on + strand at 145.452 kb on + strand at 145.453 kb on - strand at 145.453 kb on - strand at 145.453 kb on - strand at 145.453 kb on - strand at 145.454 kb on + strand at 145.454 kb on + strand at 145.454 kb on + strand at 145.454 kb on + strand at 145.455 kb on - strand at 145.455 kb on - strand at 145.455 kb on - strand at 145.460 kb on + strand at 145.630 kb on + strand, within Pf6N2E2_101 at 145.630 kb on + strand, within Pf6N2E2_101 at 145.630 kb on + strand, within Pf6N2E2_101 at 145.631 kb on - strand, within Pf6N2E2_101 at 145.907 kb on + strand, within Pf6N2E2_101 at 145.908 kb on - strand, within Pf6N2E2_101 at 145.917 kb on - strand, within Pf6N2E2_101 at 145.976 kb on + strand at 145.976 kb on + strand at 145.976 kb on + strand at 145.976 kb on + strand at 145.976 kb on + strand at 145.977 kb on - strand at 145.977 kb on - strand at 145.977 kb on - strand at 145.977 kb on - strand at 145.977 kb on - strand at 145.977 kb on - strand at 146.004 kb on - strand at 146.014 kb on - strand at 146.027 kb on + strand at 146.027 kb on + strand at 146.028 kb on - strand at 146.143 kb on - strand at 146.143 kb on - strand at 146.143 kb on - strand at 146.268 kb on + strand at 146.268 kb on + strand at 146.268 kb on + strand at 146.294 kb on + strand at 146.294 kb on + strand at 146.294 kb on + strand at 146.298 kb on + strand at 146.298 kb on + strand at 146.298 kb on + strand at 146.298 kb on + strand at 146.298 kb on + strand at 146.298 kb on + strand at 146.342 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Chloride 0.5 M remove 143,230 - Pf6N2E2_97 0.82 -0.4 143,392 + Pf6N2E2_98 0.13 +1.2 143,393 - Pf6N2E2_98 0.13 -0.7 143,629 + Pf6N2E2_98 0.90 -0.3 143,630 - +0.4 143,757 - Pf6N2E2_99 0.30 +0.6 143,757 - Pf6N2E2_99 0.30 +0.0 143,757 - Pf6N2E2_99 0.30 +0.4 143,768 - Pf6N2E2_99 0.38 -1.6 143,870 + +1.1 143,923 + +0.7 143,923 + -1.0 143,923 + +1.1 143,923 + +0.7 143,923 + +1.2 143,923 + -1.8 143,924 - -1.2 143,924 - +0.3 143,954 - -0.4 143,954 - +0.7 143,976 - +0.5 144,117 + +0.1 144,117 + -0.8 144,118 - -0.7 144,118 - -0.3 144,118 - -0.5 144,118 - -0.6 144,128 + +0.5 144,129 - +0.0 144,129 - -0.6 144,129 - +0.8 144,129 - -0.5 144,132 - +1.1 144,132 - +0.3 144,132 - -0.4 144,132 - -0.1 144,132 - -1.1 144,172 + -1.3 144,172 + +1.3 144,172 + -0.6 144,173 - +0.1 144,173 - -0.5 144,196 + +1.5 144,196 + +1.0 144,196 + +0.5 144,197 - +0.5 144,197 - -0.1 144,197 - -2.9 144,276 - Pf6N2E2_100 0.10 -0.4 144,300 + Pf6N2E2_100 0.12 +0.2 144,403 + Pf6N2E2_100 0.21 -0.0 144,404 - Pf6N2E2_100 0.21 -0.7 144,511 + Pf6N2E2_100 0.30 +0.5 144,511 + Pf6N2E2_100 0.30 -0.5 144,512 - Pf6N2E2_100 0.30 -0.9 144,512 - Pf6N2E2_100 0.30 -0.5 144,600 + Pf6N2E2_100 0.37 +0.5 144,610 + Pf6N2E2_100 0.38 -0.7 144,610 + Pf6N2E2_100 0.38 +0.3 144,610 + Pf6N2E2_100 0.38 +0.4 144,631 + Pf6N2E2_100 0.39 -0.2 144,827 - Pf6N2E2_100 0.56 +0.3 144,900 + Pf6N2E2_100 0.62 -0.2 144,901 - Pf6N2E2_100 0.62 -0.2 144,951 + Pf6N2E2_100 0.66 +0.8 144,951 + Pf6N2E2_100 0.66 -0.2 144,961 + Pf6N2E2_100 0.67 -1.0 144,979 + Pf6N2E2_100 0.68 -1.0 144,980 - Pf6N2E2_100 0.68 -0.3 144,980 - Pf6N2E2_100 0.68 -0.4 144,980 - Pf6N2E2_100 0.68 -2.9 144,985 + Pf6N2E2_100 0.69 -2.4 144,985 + Pf6N2E2_100 0.69 +0.7 144,986 - Pf6N2E2_100 0.69 -0.6 144,986 - Pf6N2E2_100 0.69 -1.1 144,986 - Pf6N2E2_100 0.69 -0.6 144,986 - Pf6N2E2_100 0.69 -0.9 144,986 - Pf6N2E2_100 0.69 -0.3 145,000 + Pf6N2E2_100 0.70 -0.6 145,001 - Pf6N2E2_100 0.70 +0.6 145,079 - Pf6N2E2_100 0.76 -0.2 145,079 - Pf6N2E2_100 0.76 +0.7 145,136 - Pf6N2E2_100 0.81 -1.0 145,352 - -0.4 145,352 - -0.5 145,391 + -0.2 145,391 + +0.5 145,391 + +1.0 145,391 + -0.3 145,391 + -1.7 145,392 - +0.1 145,392 - +0.3 145,392 - -0.4 145,392 - -1.0 145,393 + -0.3 145,393 + +0.9 145,433 + -0.3 145,434 - +1.7 145,434 - +0.2 145,436 + -1.2 145,437 - -1.0 145,437 - -1.6 145,452 + +0.2 145,452 + -0.1 145,452 + +0.3 145,452 + +0.5 145,452 + +0.9 145,452 + -0.9 145,453 - -1.7 145,453 - +0.3 145,453 - +0.1 145,453 - -0.9 145,454 + +2.0 145,454 + +2.7 145,454 + +2.0 145,454 + -0.2 145,455 - -0.5 145,455 - -0.6 145,455 - +0.0 145,460 + +0.9 145,630 + Pf6N2E2_101 0.27 -0.9 145,630 + Pf6N2E2_101 0.27 -0.5 145,630 + Pf6N2E2_101 0.27 +1.0 145,631 - Pf6N2E2_101 0.27 -1.0 145,907 + Pf6N2E2_101 0.81 +0.5 145,908 - Pf6N2E2_101 0.81 -0.1 145,917 - Pf6N2E2_101 0.83 -0.1 145,976 + -0.1 145,976 + -1.2 145,976 + -0.4 145,976 + +1.7 145,976 + +0.2 145,977 - -0.6 145,977 - -0.4 145,977 - -1.1 145,977 - -0.4 145,977 - +2.1 145,977 - -0.5 146,004 - +0.2 146,014 - -0.9 146,027 + +1.1 146,027 + +0.4 146,028 - -0.2 146,143 - +0.2 146,143 - +0.5 146,143 - -0.9 146,268 + +0.3 146,268 + -1.0 146,268 + -1.4 146,294 + -0.4 146,294 + -0.0 146,294 + +0.5 146,298 + +1.7 146,298 + -0.6 146,298 + +0.6 146,298 + -0.3 146,298 + -0.2 146,298 + +0.2 146,342 + -0.7
Or see this region's nucleotide sequence