Experiment: tungstate 0.05 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5619 and Pf6N2E2_5620 are separated by 66 nucleotides Pf6N2E2_5620 and Pf6N2E2_5621 are separated by 336 nucleotides Pf6N2E2_5621 and Pf6N2E2_5622 are separated by 7 nucleotides Pf6N2E2_5622 and Pf6N2E2_5623 are separated by 107 nucleotides Pf6N2E2_5623 and Pf6N2E2_5624 are separated by 1 nucleotides
Pf6N2E2_5619 - Putative two-component system sensor kinase, at 6,436,414 to 6,437,754
_5619
Pf6N2E2_5620 - P pilus assembly/Cpx signaling pathway, periplasmic inhibitor/zinc-resistance associated protein, at 6,437,821 to 6,438,258
_5620
Pf6N2E2_5621 - DNA-binding response regulator, at 6,438,595 to 6,439,272
_5621
Pf6N2E2_5622 - Translation initiation factor 2 (IF-2; GTPase), at 6,439,280 to 6,439,690
_5622
Pf6N2E2_5623 - YciL protein, at 6,439,798 to 6,440,097
_5623
Pf6N2E2_5624 - Intracellular septation protein IspA, at 6,440,099 to 6,440,695
_5624
Position (kb)
6438
6439
6440 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 6437.595 kb on - strand, within Pf6N2E2_5619 at 6437.757 kb on + strand at 6437.757 kb on + strand at 6437.758 kb on - strand at 6437.822 kb on + strand at 6437.822 kb on + strand at 6437.822 kb on + strand at 6437.822 kb on + strand at 6437.823 kb on - strand at 6438.029 kb on + strand, within Pf6N2E2_5620 at 6438.029 kb on + strand, within Pf6N2E2_5620 at 6438.029 kb on + strand, within Pf6N2E2_5620 at 6438.029 kb on + strand, within Pf6N2E2_5620 at 6438.030 kb on - strand, within Pf6N2E2_5620 at 6438.030 kb on - strand, within Pf6N2E2_5620 at 6438.030 kb on - strand, within Pf6N2E2_5620 at 6438.030 kb on - strand, within Pf6N2E2_5620 at 6438.367 kb on + strand at 6438.551 kb on + strand at 6438.551 kb on + strand at 6438.551 kb on + strand at 6438.552 kb on - strand at 6438.554 kb on + strand at 6438.554 kb on + strand at 6438.554 kb on + strand at 6438.555 kb on - strand at 6438.555 kb on - strand at 6438.555 kb on - strand at 6438.561 kb on + strand at 6438.561 kb on + strand at 6438.561 kb on + strand at 6438.561 kb on + strand at 6438.561 kb on + strand at 6438.561 kb on + strand at 6438.562 kb on - strand at 6438.571 kb on - strand at 6438.571 kb on - strand at 6438.578 kb on - strand at 6438.578 kb on - strand at 6438.578 kb on - strand at 6438.578 kb on - strand at 6438.580 kb on + strand at 6438.580 kb on + strand at 6438.580 kb on + strand at 6438.581 kb on - strand at 6438.605 kb on + strand at 6438.605 kb on + strand at 6438.605 kb on + strand at 6438.605 kb on + strand at 6438.605 kb on + strand at 6438.606 kb on - strand at 6438.608 kb on + strand at 6438.608 kb on + strand at 6438.608 kb on + strand at 6438.608 kb on + strand at 6438.608 kb on + strand at 6438.609 kb on - strand at 6438.611 kb on + strand at 6438.694 kb on - strand, within Pf6N2E2_5621 at 6438.710 kb on + strand, within Pf6N2E2_5621 at 6438.710 kb on + strand, within Pf6N2E2_5621 at 6438.923 kb on - strand, within Pf6N2E2_5621 at 6438.977 kb on + strand, within Pf6N2E2_5621 at 6438.978 kb on - strand, within Pf6N2E2_5621 at 6439.124 kb on - strand, within Pf6N2E2_5621 at 6439.253 kb on - strand at 6439.253 kb on - strand at 6439.259 kb on - strand at 6439.259 kb on - strand at 6439.306 kb on + strand at 6439.306 kb on + strand at 6439.306 kb on + strand at 6439.306 kb on + strand at 6439.306 kb on + strand at 6439.327 kb on + strand, within Pf6N2E2_5622 at 6439.327 kb on + strand, within Pf6N2E2_5622 at 6439.347 kb on - strand, within Pf6N2E2_5622 at 6439.665 kb on - strand at 6439.665 kb on - strand at 6439.717 kb on + strand at 6439.718 kb on - strand at 6439.718 kb on - strand at 6439.718 kb on - strand at 6439.800 kb on - strand at 6439.841 kb on + strand, within Pf6N2E2_5623 at 6439.841 kb on + strand, within Pf6N2E2_5623 at 6439.842 kb on - strand, within Pf6N2E2_5623 at 6439.987 kb on + strand, within Pf6N2E2_5623 at 6440.090 kb on + strand at 6440.090 kb on + strand at 6440.120 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction tungstate 0.05 M remove 6,437,595 - Pf6N2E2_5619 0.88 -2.1 6,437,757 + -1.9 6,437,757 + -2.5 6,437,758 - -0.2 6,437,822 + -0.5 6,437,822 + -0.7 6,437,822 + -2.3 6,437,822 + -1.7 6,437,823 - +1.0 6,438,029 + Pf6N2E2_5620 0.47 +0.4 6,438,029 + Pf6N2E2_5620 0.47 +0.2 6,438,029 + Pf6N2E2_5620 0.47 -0.9 6,438,029 + Pf6N2E2_5620 0.47 -0.3 6,438,030 - Pf6N2E2_5620 0.48 +0.4 6,438,030 - Pf6N2E2_5620 0.48 -0.8 6,438,030 - Pf6N2E2_5620 0.48 -0.6 6,438,030 - Pf6N2E2_5620 0.48 +1.2 6,438,367 + -2.6 6,438,551 + +0.6 6,438,551 + +1.2 6,438,551 + -1.9 6,438,552 - -1.2 6,438,554 + -0.3 6,438,554 + -2.8 6,438,554 + -0.8 6,438,555 - -0.2 6,438,555 - -1.2 6,438,555 - -0.4 6,438,561 + -0.7 6,438,561 + +0.3 6,438,561 + -0.2 6,438,561 + -0.6 6,438,561 + +0.2 6,438,561 + -2.7 6,438,562 - +0.3 6,438,571 - -0.1 6,438,571 - -0.7 6,438,578 - +0.2 6,438,578 - -0.8 6,438,578 - +0.3 6,438,578 - -0.1 6,438,580 + -0.9 6,438,580 + -1.6 6,438,580 + +0.5 6,438,581 - +0.4 6,438,605 + -2.1 6,438,605 + -2.0 6,438,605 + -3.0 6,438,605 + -2.2 6,438,605 + -2.9 6,438,606 - -4.1 6,438,608 + -1.9 6,438,608 + -1.6 6,438,608 + -1.3 6,438,608 + -3.0 6,438,608 + -1.9 6,438,609 - -3.5 6,438,611 + -1.1 6,438,694 - Pf6N2E2_5621 0.15 -0.4 6,438,710 + Pf6N2E2_5621 0.17 -1.8 6,438,710 + Pf6N2E2_5621 0.17 -2.8 6,438,923 - Pf6N2E2_5621 0.48 -0.7 6,438,977 + Pf6N2E2_5621 0.56 -1.9 6,438,978 - Pf6N2E2_5621 0.56 -2.9 6,439,124 - Pf6N2E2_5621 0.78 -1.0 6,439,253 - -0.8 6,439,253 - -3.2 6,439,259 - -1.4 6,439,259 - -1.8 6,439,306 + -1.4 6,439,306 + -2.2 6,439,306 + -2.7 6,439,306 + -2.3 6,439,306 + -2.2 6,439,327 + Pf6N2E2_5622 0.11 -1.4 6,439,327 + Pf6N2E2_5622 0.11 -1.7 6,439,347 - Pf6N2E2_5622 0.16 -0.3 6,439,665 - +0.6 6,439,665 - -0.1 6,439,717 + +0.5 6,439,718 - +0.4 6,439,718 - +1.3 6,439,718 - +0.4 6,439,800 - +0.3 6,439,841 + Pf6N2E2_5623 0.14 -0.5 6,439,841 + Pf6N2E2_5623 0.14 +0.3 6,439,842 - Pf6N2E2_5623 0.15 +0.7 6,439,987 + Pf6N2E2_5623 0.63 +0.3 6,440,090 + -0.7 6,440,090 + +0.4 6,440,120 - -0.1
Or see this region's nucleotide sequence