Experiment: tungstate 0.05 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4994 and Pf6N2E2_4995 are separated by 71 nucleotides Pf6N2E2_4995 and Pf6N2E2_4996 are separated by 9 nucleotides Pf6N2E2_4996 and Pf6N2E2_4997 are separated by 9 nucleotides
Pf6N2E2_4994 - Nicotinate-nucleotide adenylyltransferase (EC 2.7.7.18), at 5,760,400 to 5,761,086
_4994
Pf6N2E2_4995 - Iojap protein, at 5,761,158 to 5,761,652
_4995
Pf6N2E2_4996 - LSU m3Psi1915 methyltransferase RlmH, at 5,761,662 to 5,762,129
_4996
Pf6N2E2_4997 - Penicillin-binding protein 2 (PBP-2), at 5,762,139 to 5,764,034
_4997
Position (kb)
5761
5762
5763 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5760.691 kb on + strand, within Pf6N2E2_4994 at 5760.691 kb on + strand, within Pf6N2E2_4994 at 5760.691 kb on + strand, within Pf6N2E2_4994 at 5760.691 kb on + strand, within Pf6N2E2_4994 at 5760.691 kb on + strand, within Pf6N2E2_4994 at 5761.119 kb on + strand at 5761.137 kb on + strand at 5761.137 kb on + strand at 5761.137 kb on + strand at 5761.137 kb on + strand at 5761.728 kb on + strand, within Pf6N2E2_4996 at 5761.728 kb on + strand, within Pf6N2E2_4996 at 5761.728 kb on + strand, within Pf6N2E2_4996 at 5761.728 kb on + strand, within Pf6N2E2_4996 at 5761.728 kb on + strand, within Pf6N2E2_4996 at 5762.085 kb on + strand at 5762.085 kb on + strand at 5762.127 kb on + strand at 5762.127 kb on + strand at 5762.127 kb on + strand at 5762.127 kb on + strand at 5762.127 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction tungstate 0.05 M remove 5,760,691 + Pf6N2E2_4994 0.42 +0.4 5,760,691 + Pf6N2E2_4994 0.42 +0.0 5,760,691 + Pf6N2E2_4994 0.42 -0.0 5,760,691 + Pf6N2E2_4994 0.42 +0.7 5,760,691 + Pf6N2E2_4994 0.42 -0.1 5,761,119 + -0.3 5,761,137 + -0.0 5,761,137 + -0.7 5,761,137 + -1.1 5,761,137 + +1.3 5,761,728 + Pf6N2E2_4996 0.14 -2.9 5,761,728 + Pf6N2E2_4996 0.14 +0.3 5,761,728 + Pf6N2E2_4996 0.14 -0.1 5,761,728 + Pf6N2E2_4996 0.14 +0.3 5,761,728 + Pf6N2E2_4996 0.14 -1.8 5,762,085 + +0.9 5,762,085 + -0.0 5,762,127 + -3.3 5,762,127 + -3.2 5,762,127 + -1.1 5,762,127 + -1.9 5,762,127 + -1.6
Or see this region's nucleotide sequence