Experiment: tungstate 0.05 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3373 and Pf6N2E2_3374 are separated by 59 nucleotides Pf6N2E2_3374 and Pf6N2E2_3375 are separated by 11 nucleotides Pf6N2E2_3375 and Pf6N2E2_3376 are separated by 7 nucleotides Pf6N2E2_3376 and Pf6N2E2_3377 are separated by 84 nucleotides Pf6N2E2_3377 and Pf6N2E2_3378 are separated by 10 nucleotides Pf6N2E2_3378 and Pf6N2E2_3379 are separated by 195 nucleotides
Pf6N2E2_3373 - Outer membrane lipoprotein SmpA, a component of the essential YaeT outer-membrane protein assembly complex, at 3,905,971 to 3,906,324
_3373
Pf6N2E2_3374 - UPF0125 protein yfjF, at 3,906,384 to 3,906,671
_3374
Pf6N2E2_3375 - Putative oligoketide cyclase/lipid transport protein, similarity with yeast ubiquinone-binding protein YOL008W, at 3,906,683 to 3,907,126
_3375
Pf6N2E2_3376 - Sodium-dependent transporter family protein, at 3,907,134 to 3,908,426
_3376
Pf6N2E2_3377 - hypothetical protein, at 3,908,511 to 3,908,645
_3377
Pf6N2E2_3378 - tmRNA-binding protein SmpB, at 3,908,656 to 3,909,138
_3378
Pf6N2E2_3379 - Lactate-responsive regulator LldR in Enterobacteria, GntR family, at 3,909,334 to 3,910,101
_3379
Position (kb)
3907
3908
3909 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3906.282 kb on - strand, within Pf6N2E2_3373 at 3906.310 kb on - strand at 3906.310 kb on - strand at 3906.322 kb on + strand at 3906.322 kb on + strand at 3906.322 kb on + strand at 3906.322 kb on + strand at 3906.323 kb on - strand at 3906.323 kb on - strand at 3906.323 kb on - strand at 3906.362 kb on - strand at 3906.362 kb on - strand at 3906.385 kb on + strand at 3906.676 kb on - strand at 3906.676 kb on - strand at 3907.129 kb on - strand at 3907.129 kb on - strand at 3907.129 kb on - strand at 3907.129 kb on - strand at 3907.129 kb on - strand at 3907.129 kb on - strand at 3907.129 kb on - strand at 3907.129 kb on - strand at 3907.129 kb on - strand at 3907.162 kb on - strand at 3907.162 kb on - strand at 3907.162 kb on - strand at 3907.162 kb on - strand at 3907.187 kb on - strand at 3907.187 kb on - strand at 3907.187 kb on - strand at 3907.187 kb on - strand at 3907.187 kb on - strand at 3907.187 kb on - strand at 3907.219 kb on - strand at 3907.379 kb on - strand, within Pf6N2E2_3376 at 3907.429 kb on - strand, within Pf6N2E2_3376 at 3907.429 kb on - strand, within Pf6N2E2_3376 at 3907.429 kb on - strand, within Pf6N2E2_3376 at 3907.429 kb on - strand, within Pf6N2E2_3376 at 3907.429 kb on - strand, within Pf6N2E2_3376 at 3907.429 kb on - strand, within Pf6N2E2_3376 at 3907.429 kb on - strand, within Pf6N2E2_3376 at 3907.429 kb on - strand, within Pf6N2E2_3376 at 3907.429 kb on - strand, within Pf6N2E2_3376 at 3907.429 kb on - strand, within Pf6N2E2_3376 at 3907.429 kb on - strand, within Pf6N2E2_3376 at 3907.429 kb on - strand, within Pf6N2E2_3376 at 3907.429 kb on - strand, within Pf6N2E2_3376 at 3907.431 kb on - strand, within Pf6N2E2_3376 at 3907.431 kb on - strand, within Pf6N2E2_3376 at 3907.431 kb on - strand, within Pf6N2E2_3376 at 3907.431 kb on - strand, within Pf6N2E2_3376 at 3907.431 kb on - strand, within Pf6N2E2_3376 at 3907.431 kb on - strand, within Pf6N2E2_3376 at 3907.431 kb on - strand, within Pf6N2E2_3376 at 3907.431 kb on - strand, within Pf6N2E2_3376 at 3907.431 kb on - strand, within Pf6N2E2_3376 at 3907.431 kb on - strand, within Pf6N2E2_3376 at 3907.431 kb on - strand, within Pf6N2E2_3376 at 3907.431 kb on - strand, within Pf6N2E2_3376 at 3907.431 kb on - strand, within Pf6N2E2_3376 at 3907.529 kb on - strand at 3907.529 kb on - strand, within Pf6N2E2_3376 at 3907.529 kb on - strand, within Pf6N2E2_3376 at 3907.799 kb on - strand, within Pf6N2E2_3376 at 3907.799 kb on - strand, within Pf6N2E2_3376 at 3907.799 kb on - strand, within Pf6N2E2_3376 at 3907.799 kb on - strand, within Pf6N2E2_3376 at 3907.799 kb on - strand, within Pf6N2E2_3376 at 3907.799 kb on - strand, within Pf6N2E2_3376 at 3907.799 kb on - strand, within Pf6N2E2_3376 at 3907.808 kb on - strand, within Pf6N2E2_3376 at 3907.808 kb on - strand, within Pf6N2E2_3376 at 3907.808 kb on - strand, within Pf6N2E2_3376 at 3907.808 kb on - strand, within Pf6N2E2_3376 at 3908.174 kb on - strand, within Pf6N2E2_3376 at 3908.174 kb on - strand, within Pf6N2E2_3376 at 3908.174 kb on - strand, within Pf6N2E2_3376 at 3908.174 kb on - strand, within Pf6N2E2_3376 at 3908.202 kb on - strand, within Pf6N2E2_3376 at 3908.202 kb on - strand, within Pf6N2E2_3376 at 3908.203 kb on + strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.204 kb on - strand, within Pf6N2E2_3376 at 3908.290 kb on - strand, within Pf6N2E2_3376 at 3908.429 kb on - strand at 3908.429 kb on - strand at 3908.429 kb on - strand at 3908.445 kb on - strand at 3908.445 kb on - strand at 3908.445 kb on - strand at 3908.445 kb on - strand at 3908.445 kb on - strand at 3908.482 kb on - strand at 3908.482 kb on - strand at 3908.482 kb on - strand at 3908.641 kb on + strand at 3909.410 kb on + strand at 3909.410 kb on + strand at 3909.410 kb on + strand at 3909.410 kb on + strand at 3909.410 kb on + strand at 3909.410 kb on + strand at 3909.410 kb on + strand at 3909.410 kb on + strand at 3909.410 kb on + strand at 3909.410 kb on + strand at 3909.411 kb on - strand, within Pf6N2E2_3379 at 3909.411 kb on - strand, within Pf6N2E2_3379 at 3909.411 kb on - strand, within Pf6N2E2_3379 at 3909.411 kb on - strand, within Pf6N2E2_3379 at 3909.411 kb on - strand, within Pf6N2E2_3379 at 3909.411 kb on - strand, within Pf6N2E2_3379 at 3909.417 kb on + strand, within Pf6N2E2_3379 at 3909.417 kb on + strand, within Pf6N2E2_3379 at 3909.417 kb on + strand, within Pf6N2E2_3379 at 3909.417 kb on + strand, within Pf6N2E2_3379 at 3909.417 kb on + strand, within Pf6N2E2_3379 at 3909.417 kb on + strand, within Pf6N2E2_3379 at 3909.417 kb on + strand, within Pf6N2E2_3379 at 3909.417 kb on + strand, within Pf6N2E2_3379 at 3909.418 kb on - strand, within Pf6N2E2_3379 at 3909.418 kb on - strand, within Pf6N2E2_3379 at 3909.418 kb on - strand, within Pf6N2E2_3379 at 3909.418 kb on - strand, within Pf6N2E2_3379 at 3909.418 kb on - strand, within Pf6N2E2_3379 at 3909.418 kb on - strand, within Pf6N2E2_3379
Per-strain Table
Position Strand Gene LocusTag Fraction tungstate 0.05 M remove 3,906,282 - Pf6N2E2_3373 0.88 +0.5 3,906,310 - -1.1 3,906,310 - +0.3 3,906,322 + -2.4 3,906,322 + -0.0 3,906,322 + +0.5 3,906,322 + +1.0 3,906,323 - +0.2 3,906,323 - +0.1 3,906,323 - -0.2 3,906,362 - +1.3 3,906,362 - -0.1 3,906,385 + +0.3 3,906,676 - +0.3 3,906,676 - -0.5 3,907,129 - -1.6 3,907,129 - -0.8 3,907,129 - -0.2 3,907,129 - +0.3 3,907,129 - +0.5 3,907,129 - -0.4 3,907,129 - -1.7 3,907,129 - +0.0 3,907,129 - -0.2 3,907,162 - +1.0 3,907,162 - +0.3 3,907,162 - +1.4 3,907,162 - +1.3 3,907,187 - +0.3 3,907,187 - +0.8 3,907,187 - -0.3 3,907,187 - -0.3 3,907,187 - +1.3 3,907,187 - +0.1 3,907,219 - -0.3 3,907,379 - Pf6N2E2_3376 0.19 +0.8 3,907,429 - Pf6N2E2_3376 0.23 -0.2 3,907,429 - Pf6N2E2_3376 0.23 -0.3 3,907,429 - Pf6N2E2_3376 0.23 +0.8 3,907,429 - Pf6N2E2_3376 0.23 +0.4 3,907,429 - Pf6N2E2_3376 0.23 +0.4 3,907,429 - Pf6N2E2_3376 0.23 +1.3 3,907,429 - Pf6N2E2_3376 0.23 +0.6 3,907,429 - Pf6N2E2_3376 0.23 +0.2 3,907,429 - Pf6N2E2_3376 0.23 +0.4 3,907,429 - Pf6N2E2_3376 0.23 -0.9 3,907,429 - Pf6N2E2_3376 0.23 +1.2 3,907,429 - Pf6N2E2_3376 0.23 -0.4 3,907,429 - Pf6N2E2_3376 0.23 +1.8 3,907,431 - Pf6N2E2_3376 0.23 -0.2 3,907,431 - Pf6N2E2_3376 0.23 -1.0 3,907,431 - Pf6N2E2_3376 0.23 +0.3 3,907,431 - Pf6N2E2_3376 0.23 +0.5 3,907,431 - Pf6N2E2_3376 0.23 +0.8 3,907,431 - Pf6N2E2_3376 0.23 +1.0 3,907,431 - Pf6N2E2_3376 0.23 +1.9 3,907,431 - Pf6N2E2_3376 0.23 +0.4 3,907,431 - Pf6N2E2_3376 0.23 +0.9 3,907,431 - Pf6N2E2_3376 0.23 +0.2 3,907,431 - Pf6N2E2_3376 0.23 +0.6 3,907,431 - Pf6N2E2_3376 0.23 -0.0 3,907,431 - Pf6N2E2_3376 0.23 +0.9 3,907,529 - +0.3 3,907,529 - Pf6N2E2_3376 0.31 +0.0 3,907,529 - Pf6N2E2_3376 0.31 +1.3 3,907,799 - Pf6N2E2_3376 0.51 +0.1 3,907,799 - Pf6N2E2_3376 0.51 +1.1 3,907,799 - Pf6N2E2_3376 0.51 +1.6 3,907,799 - Pf6N2E2_3376 0.51 +0.4 3,907,799 - Pf6N2E2_3376 0.51 +0.9 3,907,799 - Pf6N2E2_3376 0.51 +0.6 3,907,799 - Pf6N2E2_3376 0.51 +0.2 3,907,808 - Pf6N2E2_3376 0.52 -0.7 3,907,808 - Pf6N2E2_3376 0.52 +1.0 3,907,808 - Pf6N2E2_3376 0.52 +0.1 3,907,808 - Pf6N2E2_3376 0.52 +0.2 3,908,174 - Pf6N2E2_3376 0.80 +0.8 3,908,174 - Pf6N2E2_3376 0.80 +0.7 3,908,174 - Pf6N2E2_3376 0.80 +1.2 3,908,174 - Pf6N2E2_3376 0.80 +0.7 3,908,202 - Pf6N2E2_3376 0.83 -1.9 3,908,202 - Pf6N2E2_3376 0.83 -0.6 3,908,203 + Pf6N2E2_3376 0.83 -0.1 3,908,204 - Pf6N2E2_3376 0.83 +0.3 3,908,204 - Pf6N2E2_3376 0.83 -0.2 3,908,204 - Pf6N2E2_3376 0.83 +0.7 3,908,204 - Pf6N2E2_3376 0.83 +0.6 3,908,204 - Pf6N2E2_3376 0.83 +1.9 3,908,204 - Pf6N2E2_3376 0.83 +0.9 3,908,204 - Pf6N2E2_3376 0.83 -0.1 3,908,204 - Pf6N2E2_3376 0.83 +1.1 3,908,204 - Pf6N2E2_3376 0.83 +1.1 3,908,204 - Pf6N2E2_3376 0.83 +0.3 3,908,204 - Pf6N2E2_3376 0.83 -0.1 3,908,204 - Pf6N2E2_3376 0.83 -1.4 3,908,290 - Pf6N2E2_3376 0.89 -0.8 3,908,429 - +0.7 3,908,429 - +0.7 3,908,429 - -0.5 3,908,445 - +0.6 3,908,445 - +0.0 3,908,445 - +0.6 3,908,445 - -0.2 3,908,445 - +0.6 3,908,482 - -0.2 3,908,482 - -0.5 3,908,482 - +0.4 3,908,641 + +1.1 3,909,410 + -1.3 3,909,410 + +1.2 3,909,410 + +1.7 3,909,410 + -0.1 3,909,410 + -0.1 3,909,410 + +0.5 3,909,410 + +1.4 3,909,410 + -1.2 3,909,410 + -3.9 3,909,410 + -1.4 3,909,411 - Pf6N2E2_3379 0.10 -0.6 3,909,411 - Pf6N2E2_3379 0.10 +1.1 3,909,411 - Pf6N2E2_3379 0.10 -1.5 3,909,411 - Pf6N2E2_3379 0.10 +0.4 3,909,411 - Pf6N2E2_3379 0.10 +1.5 3,909,411 - Pf6N2E2_3379 0.10 +1.1 3,909,417 + Pf6N2E2_3379 0.11 +0.2 3,909,417 + Pf6N2E2_3379 0.11 +0.8 3,909,417 + Pf6N2E2_3379 0.11 -1.0 3,909,417 + Pf6N2E2_3379 0.11 -0.7 3,909,417 + Pf6N2E2_3379 0.11 +0.3 3,909,417 + Pf6N2E2_3379 0.11 -0.4 3,909,417 + Pf6N2E2_3379 0.11 -1.4 3,909,417 + Pf6N2E2_3379 0.11 -0.3 3,909,418 - Pf6N2E2_3379 0.11 -0.3 3,909,418 - Pf6N2E2_3379 0.11 -0.7 3,909,418 - Pf6N2E2_3379 0.11 -0.3 3,909,418 - Pf6N2E2_3379 0.11 -0.1 3,909,418 - Pf6N2E2_3379 0.11 -0.2 3,909,418 - Pf6N2E2_3379 0.11 +0.6
Or see this region's nucleotide sequence