Experiment: tungstate 0.05 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_137 and Pf6N2E2_138 are separated by 30 nucleotides Pf6N2E2_138 and Pf6N2E2_139 are separated by 435 nucleotides
Pf6N2E2_137 - Glycolate dehydrogenase (EC 1.1.99.14), subunit GlcD, at 186,099 to 187,634
_137
Pf6N2E2_138 - Alpha/beta hydrolase fold (EC 3.8.1.5), at 187,665 to 188,594
_138
Pf6N2E2_139 - Two-component response regulator, at 189,030 to 189,977
_139
Position (kb) 187
188
189 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 187.085 kb on - strand, within Pf6N2E2_137 at 187.168 kb on + strand, within Pf6N2E2_137 at 187.168 kb on + strand, within Pf6N2E2_137 at 187.168 kb on + strand, within Pf6N2E2_137 at 187.171 kb on + strand, within Pf6N2E2_137 at 187.171 kb on + strand, within Pf6N2E2_137 at 187.172 kb on - strand, within Pf6N2E2_137 at 187.293 kb on + strand, within Pf6N2E2_137 at 187.293 kb on + strand, within Pf6N2E2_137 at 187.294 kb on - strand, within Pf6N2E2_137 at 187.359 kb on + strand, within Pf6N2E2_137 at 187.360 kb on - strand, within Pf6N2E2_137 at 187.468 kb on + strand, within Pf6N2E2_137 at 187.585 kb on + strand at 187.585 kb on + strand at 187.634 kb on + strand at 187.634 kb on + strand at 187.634 kb on + strand at 187.634 kb on + strand at 187.634 kb on + strand at 187.634 kb on + strand at 187.634 kb on + strand at 187.634 kb on + strand at 187.634 kb on + strand at 187.634 kb on + strand at 187.634 kb on + strand at 187.635 kb on - strand at 187.635 kb on - strand at 187.635 kb on - strand at 187.635 kb on - strand at 187.635 kb on - strand at 187.635 kb on - strand at 187.635 kb on - strand at 187.840 kb on - strand, within Pf6N2E2_138 at 187.840 kb on - strand, within Pf6N2E2_138 at 187.840 kb on - strand, within Pf6N2E2_138 at 188.005 kb on + strand, within Pf6N2E2_138 at 188.006 kb on - strand, within Pf6N2E2_138 at 188.171 kb on - strand, within Pf6N2E2_138 at 188.263 kb on + strand, within Pf6N2E2_138 at 188.326 kb on + strand, within Pf6N2E2_138 at 188.381 kb on - strand, within Pf6N2E2_138 at 188.515 kb on + strand at 188.540 kb on - strand at 188.540 kb on - strand at 188.655 kb on + strand at 188.656 kb on - strand at 188.656 kb on - strand at 188.656 kb on - strand at 188.656 kb on - strand at 188.662 kb on + strand at 188.662 kb on + strand at 188.699 kb on - strand at 188.699 kb on - strand at 188.715 kb on + strand at 188.715 kb on + strand at 188.715 kb on + strand at 188.715 kb on + strand at 188.716 kb on - strand at 188.716 kb on - strand at 188.716 kb on - strand at 188.723 kb on - strand at 188.723 kb on - strand at 188.723 kb on - strand at 188.747 kb on + strand at 188.747 kb on + strand at 188.747 kb on + strand at 188.748 kb on - strand at 188.748 kb on - strand at 188.748 kb on - strand at 188.748 kb on - strand at 188.748 kb on - strand at 188.748 kb on - strand at 188.748 kb on - strand at 188.748 kb on - strand at 188.789 kb on + strand at 188.789 kb on + strand at 188.789 kb on + strand at 188.790 kb on - strand at 188.833 kb on + strand at 188.833 kb on + strand at 188.937 kb on + strand at 188.937 kb on + strand at 188.937 kb on + strand at 188.937 kb on + strand at 188.937 kb on + strand at 188.938 kb on - strand at 188.938 kb on - strand at 188.938 kb on - strand at 188.938 kb on - strand at 188.938 kb on - strand at 188.938 kb on - strand at 188.939 kb on + strand at 188.939 kb on + strand at 188.939 kb on + strand at 188.939 kb on + strand at 188.939 kb on + strand at 188.939 kb on + strand at 188.939 kb on + strand at 188.939 kb on + strand at 188.939 kb on + strand at 188.940 kb on - strand at 188.940 kb on - strand at 188.940 kb on - strand at 188.940 kb on - strand at 188.940 kb on - strand at 188.940 kb on - strand at 188.940 kb on - strand at 188.941 kb on + strand at 188.942 kb on - strand at 189.066 kb on + strand at 189.118 kb on + strand at 189.119 kb on - strand at 189.336 kb on + strand, within Pf6N2E2_139 at 189.336 kb on + strand, within Pf6N2E2_139 at 189.336 kb on + strand, within Pf6N2E2_139 at 189.336 kb on + strand, within Pf6N2E2_139 at 189.336 kb on + strand, within Pf6N2E2_139 at 189.336 kb on + strand, within Pf6N2E2_139 at 189.336 kb on + strand, within Pf6N2E2_139 at 189.336 kb on + strand, within Pf6N2E2_139 at 189.337 kb on - strand, within Pf6N2E2_139 at 189.337 kb on - strand, within Pf6N2E2_139 at 189.337 kb on - strand, within Pf6N2E2_139 at 189.337 kb on - strand, within Pf6N2E2_139 at 189.337 kb on - strand, within Pf6N2E2_139 at 189.337 kb on - strand, within Pf6N2E2_139 at 189.337 kb on - strand, within Pf6N2E2_139 at 189.337 kb on - strand, within Pf6N2E2_139 at 189.337 kb on - strand, within Pf6N2E2_139 at 189.337 kb on - strand, within Pf6N2E2_139 at 189.337 kb on - strand, within Pf6N2E2_139 at 189.337 kb on - strand, within Pf6N2E2_139 at 189.402 kb on + strand, within Pf6N2E2_139 at 189.402 kb on + strand, within Pf6N2E2_139 at 189.402 kb on + strand, within Pf6N2E2_139 at 189.403 kb on - strand, within Pf6N2E2_139 at 189.403 kb on - strand, within Pf6N2E2_139 at 189.403 kb on - strand, within Pf6N2E2_139
Per-strain Table
Position Strand Gene LocusTag Fraction tungstate 0.05 M remove 187,085 - Pf6N2E2_137 0.64 -0.1 187,168 + Pf6N2E2_137 0.70 +1.0 187,168 + Pf6N2E2_137 0.70 +0.1 187,168 + Pf6N2E2_137 0.70 +0.5 187,171 + Pf6N2E2_137 0.70 -0.7 187,171 + Pf6N2E2_137 0.70 +0.2 187,172 - Pf6N2E2_137 0.70 -0.1 187,293 + Pf6N2E2_137 0.78 -0.5 187,293 + Pf6N2E2_137 0.78 -1.3 187,294 - Pf6N2E2_137 0.78 +0.2 187,359 + Pf6N2E2_137 0.82 -0.1 187,360 - Pf6N2E2_137 0.82 +0.6 187,468 + Pf6N2E2_137 0.89 -0.2 187,585 + -0.5 187,585 + -0.7 187,634 + -0.6 187,634 + +0.4 187,634 + -0.1 187,634 + +0.2 187,634 + +0.3 187,634 + +0.2 187,634 + -0.1 187,634 + -0.7 187,634 + +0.2 187,634 + -0.3 187,634 + +0.0 187,635 - -0.3 187,635 - +0.3 187,635 - +1.0 187,635 - -1.3 187,635 - +0.4 187,635 - -0.5 187,635 - -0.9 187,840 - Pf6N2E2_138 0.19 -0.1 187,840 - Pf6N2E2_138 0.19 -0.3 187,840 - Pf6N2E2_138 0.19 +1.5 188,005 + Pf6N2E2_138 0.37 +0.3 188,006 - Pf6N2E2_138 0.37 -3.0 188,171 - Pf6N2E2_138 0.54 -0.8 188,263 + Pf6N2E2_138 0.64 -2.5 188,326 + Pf6N2E2_138 0.71 -0.8 188,381 - Pf6N2E2_138 0.77 -0.5 188,515 + -0.1 188,540 - -0.8 188,540 - -0.1 188,655 + +0.2 188,656 - +1.3 188,656 - +0.1 188,656 - -0.1 188,656 - -0.4 188,662 + -0.7 188,662 + -0.8 188,699 - -0.1 188,699 - -0.1 188,715 + -1.2 188,715 + -0.5 188,715 + +1.0 188,715 + -1.8 188,716 - -0.4 188,716 - +0.7 188,716 - -0.0 188,723 - -0.1 188,723 - +0.8 188,723 - -0.0 188,747 + -0.4 188,747 + -0.3 188,747 + -0.3 188,748 - +0.0 188,748 - -1.0 188,748 - +1.0 188,748 - +1.1 188,748 - +0.1 188,748 - -0.2 188,748 - +0.8 188,748 - +1.8 188,789 + +1.2 188,789 + -0.0 188,789 + +2.5 188,790 - -0.3 188,833 + +1.5 188,833 + -0.8 188,937 + +1.5 188,937 + -0.6 188,937 + -1.1 188,937 + -0.4 188,937 + -1.1 188,938 - -0.3 188,938 - +1.6 188,938 - -0.3 188,938 - +1.3 188,938 - -0.2 188,938 - +2.5 188,939 + -0.1 188,939 + -1.4 188,939 + -0.1 188,939 + -1.4 188,939 + +1.6 188,939 + -0.6 188,939 + -1.1 188,939 + +1.2 188,939 + -0.8 188,940 - -0.2 188,940 - -1.3 188,940 - -0.8 188,940 - +0.7 188,940 - -0.5 188,940 - +1.6 188,940 - +1.9 188,941 + -0.8 188,942 - -0.1 189,066 + +0.1 189,118 + +0.1 189,119 - -0.8 189,336 + Pf6N2E2_139 0.32 +0.6 189,336 + Pf6N2E2_139 0.32 +0.3 189,336 + Pf6N2E2_139 0.32 +0.4 189,336 + Pf6N2E2_139 0.32 -0.7 189,336 + Pf6N2E2_139 0.32 -0.8 189,336 + Pf6N2E2_139 0.32 -0.3 189,336 + Pf6N2E2_139 0.32 +0.7 189,336 + Pf6N2E2_139 0.32 -0.8 189,337 - Pf6N2E2_139 0.32 +1.0 189,337 - Pf6N2E2_139 0.32 +1.2 189,337 - Pf6N2E2_139 0.32 -0.1 189,337 - Pf6N2E2_139 0.32 -0.6 189,337 - Pf6N2E2_139 0.32 +0.4 189,337 - Pf6N2E2_139 0.32 +0.5 189,337 - Pf6N2E2_139 0.32 +0.6 189,337 - Pf6N2E2_139 0.32 -0.8 189,337 - Pf6N2E2_139 0.32 +0.8 189,337 - Pf6N2E2_139 0.32 -0.5 189,337 - Pf6N2E2_139 0.32 +0.4 189,337 - Pf6N2E2_139 0.32 -0.3 189,402 + Pf6N2E2_139 0.39 -0.5 189,402 + Pf6N2E2_139 0.39 +0.5 189,402 + Pf6N2E2_139 0.39 -0.4 189,403 - Pf6N2E2_139 0.39 -1.0 189,403 - Pf6N2E2_139 0.39 +0.7 189,403 - Pf6N2E2_139 0.39 +0.7
Or see this region's nucleotide sequence