Experiment: tungstate 0.05 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1214 and Pf6N2E2_1215 are separated by 168 nucleotides Pf6N2E2_1215 and Pf6N2E2_1216 are separated by 51 nucleotides Pf6N2E2_1216 and Pf6N2E2_1217 overlap by 4 nucleotides
Pf6N2E2_1214 - C-terminal domain of CinA type S; Protein Implicated in DNA repair function with RecA and MutS, at 1,419,804 to 1,420,304
_1214
Pf6N2E2_1215 - probable membrane protein YPO2362, at 1,420,473 to 1,421,084
_1215
Pf6N2E2_1216 - ATP-dependent DNA ligase (EC 6.5.1.1) clustered with Ku protein, LigD, at 1,421,136 to 1,423,772
_1216
Pf6N2E2_1217 - Gll2069 protein, at 1,423,769 to 1,424,920
_1217
Position (kb)
1421
1422
1423
1424 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1420.157 kb on - strand, within Pf6N2E2_1214 at 1420.276 kb on + strand at 1420.277 kb on - strand at 1420.277 kb on - strand at 1420.277 kb on - strand at 1420.528 kb on + strand at 1420.528 kb on + strand at 1420.528 kb on + strand at 1420.529 kb on - strand at 1420.529 kb on - strand at 1420.529 kb on - strand at 1420.529 kb on - strand at 1420.560 kb on + strand, within Pf6N2E2_1215 at 1420.601 kb on - strand, within Pf6N2E2_1215 at 1420.702 kb on - strand, within Pf6N2E2_1215 at 1420.702 kb on - strand, within Pf6N2E2_1215 at 1420.810 kb on - strand, within Pf6N2E2_1215 at 1421.046 kb on + strand at 1421.181 kb on - strand at 1421.383 kb on + strand at 1421.578 kb on - strand, within Pf6N2E2_1216 at 1421.938 kb on + strand, within Pf6N2E2_1216 at 1421.938 kb on + strand, within Pf6N2E2_1216 at 1421.939 kb on - strand, within Pf6N2E2_1216 at 1422.361 kb on + strand, within Pf6N2E2_1216 at 1422.361 kb on + strand, within Pf6N2E2_1216 at 1422.565 kb on + strand, within Pf6N2E2_1216 at 1422.565 kb on + strand, within Pf6N2E2_1216 at 1422.565 kb on + strand, within Pf6N2E2_1216 at 1422.565 kb on + strand, within Pf6N2E2_1216 at 1422.565 kb on + strand, within Pf6N2E2_1216 at 1422.565 kb on + strand, within Pf6N2E2_1216 at 1422.565 kb on + strand, within Pf6N2E2_1216 at 1422.565 kb on + strand, within Pf6N2E2_1216 at 1422.566 kb on - strand, within Pf6N2E2_1216 at 1422.566 kb on - strand, within Pf6N2E2_1216 at 1422.566 kb on - strand, within Pf6N2E2_1216 at 1422.566 kb on - strand, within Pf6N2E2_1216 at 1422.566 kb on - strand, within Pf6N2E2_1216 at 1422.757 kb on + strand, within Pf6N2E2_1216 at 1422.775 kb on + strand, within Pf6N2E2_1216 at 1422.775 kb on + strand, within Pf6N2E2_1216 at 1422.775 kb on + strand, within Pf6N2E2_1216 at 1422.776 kb on - strand, within Pf6N2E2_1216 at 1423.021 kb on + strand, within Pf6N2E2_1216 at 1423.022 kb on - strand, within Pf6N2E2_1216 at 1423.022 kb on - strand, within Pf6N2E2_1216 at 1423.022 kb on - strand, within Pf6N2E2_1216 at 1423.258 kb on + strand, within Pf6N2E2_1216 at 1423.507 kb on + strand, within Pf6N2E2_1216 at 1423.747 kb on + strand at 1423.747 kb on + strand at 1423.747 kb on + strand at 1423.748 kb on - strand at 1423.748 kb on - strand at 1423.748 kb on - strand at 1423.748 kb on - strand at 1423.942 kb on + strand, within Pf6N2E2_1217 at 1423.943 kb on - strand, within Pf6N2E2_1217 at 1423.961 kb on + strand, within Pf6N2E2_1217 at 1424.136 kb on + strand, within Pf6N2E2_1217 at 1424.137 kb on - strand, within Pf6N2E2_1217 at 1424.137 kb on - strand, within Pf6N2E2_1217 at 1424.277 kb on - strand, within Pf6N2E2_1217 at 1424.337 kb on + strand, within Pf6N2E2_1217 at 1424.433 kb on + strand, within Pf6N2E2_1217 at 1424.433 kb on + strand, within Pf6N2E2_1217 at 1424.549 kb on - strand, within Pf6N2E2_1217 at 1424.590 kb on - strand, within Pf6N2E2_1217 at 1424.590 kb on - strand, within Pf6N2E2_1217 at 1424.590 kb on - strand, within Pf6N2E2_1217
Per-strain Table
Position Strand Gene LocusTag Fraction tungstate 0.05 M remove 1,420,157 - Pf6N2E2_1214 0.70 +1.0 1,420,276 + +1.4 1,420,277 - +0.2 1,420,277 - +0.4 1,420,277 - +0.9 1,420,528 + +0.3 1,420,528 + +1.1 1,420,528 + +0.4 1,420,529 - -0.2 1,420,529 - -1.3 1,420,529 - +0.8 1,420,529 - -0.0 1,420,560 + Pf6N2E2_1215 0.14 -1.8 1,420,601 - Pf6N2E2_1215 0.21 -0.2 1,420,702 - Pf6N2E2_1215 0.37 +0.4 1,420,702 - Pf6N2E2_1215 0.37 +0.8 1,420,810 - Pf6N2E2_1215 0.55 +0.4 1,421,046 + +0.2 1,421,181 - +0.3 1,421,383 + -0.4 1,421,578 - Pf6N2E2_1216 0.17 +0.2 1,421,938 + Pf6N2E2_1216 0.30 -0.1 1,421,938 + Pf6N2E2_1216 0.30 +0.4 1,421,939 - Pf6N2E2_1216 0.30 -0.5 1,422,361 + Pf6N2E2_1216 0.46 -1.3 1,422,361 + Pf6N2E2_1216 0.46 -1.2 1,422,565 + Pf6N2E2_1216 0.54 -0.2 1,422,565 + Pf6N2E2_1216 0.54 +1.6 1,422,565 + Pf6N2E2_1216 0.54 +2.0 1,422,565 + Pf6N2E2_1216 0.54 -2.1 1,422,565 + Pf6N2E2_1216 0.54 -0.6 1,422,565 + Pf6N2E2_1216 0.54 -0.6 1,422,565 + Pf6N2E2_1216 0.54 +0.4 1,422,565 + Pf6N2E2_1216 0.54 +1.6 1,422,566 - Pf6N2E2_1216 0.54 -0.2 1,422,566 - Pf6N2E2_1216 0.54 -1.8 1,422,566 - Pf6N2E2_1216 0.54 -1.2 1,422,566 - Pf6N2E2_1216 0.54 -0.2 1,422,566 - Pf6N2E2_1216 0.54 -2.2 1,422,757 + Pf6N2E2_1216 0.61 -1.3 1,422,775 + Pf6N2E2_1216 0.62 +2.2 1,422,775 + Pf6N2E2_1216 0.62 -0.8 1,422,775 + Pf6N2E2_1216 0.62 +0.3 1,422,776 - Pf6N2E2_1216 0.62 +1.4 1,423,021 + Pf6N2E2_1216 0.71 -0.0 1,423,022 - Pf6N2E2_1216 0.72 -0.7 1,423,022 - Pf6N2E2_1216 0.72 -1.1 1,423,022 - Pf6N2E2_1216 0.72 -0.1 1,423,258 + Pf6N2E2_1216 0.80 +0.4 1,423,507 + Pf6N2E2_1216 0.90 -0.2 1,423,747 + -0.3 1,423,747 + +0.6 1,423,747 + -0.1 1,423,748 - +0.1 1,423,748 - +1.4 1,423,748 - -0.2 1,423,748 - +1.4 1,423,942 + Pf6N2E2_1217 0.15 +1.0 1,423,943 - Pf6N2E2_1217 0.15 -1.0 1,423,961 + Pf6N2E2_1217 0.17 -0.5 1,424,136 + Pf6N2E2_1217 0.32 -0.8 1,424,137 - Pf6N2E2_1217 0.32 +0.5 1,424,137 - Pf6N2E2_1217 0.32 -2.0 1,424,277 - Pf6N2E2_1217 0.44 -1.1 1,424,337 + Pf6N2E2_1217 0.49 +1.4 1,424,433 + Pf6N2E2_1217 0.58 +0.4 1,424,433 + Pf6N2E2_1217 0.58 +0.4 1,424,549 - Pf6N2E2_1217 0.68 +0.8 1,424,590 - Pf6N2E2_1217 0.71 +0.4 1,424,590 - Pf6N2E2_1217 0.71 +0.3 1,424,590 - Pf6N2E2_1217 0.71 +0.2
Or see this region's nucleotide sequence