Experiment: tungstate 0.05 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_104 and Pf6N2E2_105 overlap by 4 nucleotides Pf6N2E2_105 and Pf6N2E2_106 are separated by 10 nucleotides Pf6N2E2_106 and Pf6N2E2_107 are separated by 10 nucleotides
Pf6N2E2_104 - Glycerol-3-phosphate ABC transporter, ATP-binding protein UgpC (TC 3.A.1.1.3), at 149,638 to 150,747
_104
Pf6N2E2_105 - Glycerol-3-phosphate ABC transporter, permease protein UgpA (TC 3.A.1.1.3), at 150,744 to 151,610
_105
Pf6N2E2_106 - Glycerol-3-phosphate ABC transporter, permease protein UgpE (TC 3.A.1.1.3), at 151,621 to 152,421
_106
Pf6N2E2_107 - Probable transmembrane protein, at 152,432 to 152,704
_107
Position (kb) 150
151
152 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 149.914 kb on + strand, within Pf6N2E2_104 at 149.914 kb on + strand, within Pf6N2E2_104 at 149.914 kb on + strand, within Pf6N2E2_104 at 149.915 kb on - strand, within Pf6N2E2_104 at 149.915 kb on - strand, within Pf6N2E2_104 at 149.915 kb on - strand, within Pf6N2E2_104 at 149.942 kb on + strand, within Pf6N2E2_104 at 149.943 kb on - strand, within Pf6N2E2_104 at 149.943 kb on - strand, within Pf6N2E2_104 at 150.223 kb on + strand, within Pf6N2E2_104 at 150.224 kb on - strand, within Pf6N2E2_104 at 150.280 kb on + strand, within Pf6N2E2_104 at 150.281 kb on - strand, within Pf6N2E2_104 at 150.281 kb on - strand, within Pf6N2E2_104 at 150.532 kb on + strand, within Pf6N2E2_104 at 150.533 kb on - strand, within Pf6N2E2_104 at 150.583 kb on + strand, within Pf6N2E2_104 at 150.583 kb on + strand, within Pf6N2E2_104 at 150.584 kb on - strand, within Pf6N2E2_104 at 150.584 kb on - strand, within Pf6N2E2_104 at 150.627 kb on - strand, within Pf6N2E2_104 at 150.667 kb on + strand at 150.667 kb on + strand at 150.667 kb on + strand at 150.701 kb on - strand at 150.712 kb on + strand at 150.712 kb on + strand at 150.879 kb on + strand, within Pf6N2E2_105 at 151.167 kb on + strand, within Pf6N2E2_105 at 151.167 kb on + strand, within Pf6N2E2_105 at 151.168 kb on - strand, within Pf6N2E2_105 at 151.168 kb on - strand, within Pf6N2E2_105 at 151.173 kb on + strand, within Pf6N2E2_105 at 151.173 kb on + strand, within Pf6N2E2_105 at 151.174 kb on - strand, within Pf6N2E2_105 at 151.174 kb on - strand, within Pf6N2E2_105 at 151.174 kb on - strand, within Pf6N2E2_105 at 151.258 kb on - strand, within Pf6N2E2_105 at 151.287 kb on + strand, within Pf6N2E2_105 at 151.288 kb on - strand, within Pf6N2E2_105 at 151.288 kb on - strand, within Pf6N2E2_105 at 151.290 kb on + strand, within Pf6N2E2_105 at 151.290 kb on + strand at 151.290 kb on + strand, within Pf6N2E2_105 at 151.291 kb on - strand, within Pf6N2E2_105 at 151.413 kb on + strand, within Pf6N2E2_105 at 151.414 kb on - strand, within Pf6N2E2_105 at 151.540 kb on - strand at 151.540 kb on - strand at 151.540 kb on - strand at 151.540 kb on - strand at 151.575 kb on + strand at 151.575 kb on + strand at 151.654 kb on + strand at 151.655 kb on - strand at 151.655 kb on - strand at 151.655 kb on - strand at 151.681 kb on + strand at 151.695 kb on + strand at 151.695 kb on + strand at 151.696 kb on - strand at 151.768 kb on + strand, within Pf6N2E2_106 at 151.768 kb on + strand, within Pf6N2E2_106 at 151.768 kb on + strand, within Pf6N2E2_106 at 151.769 kb on - strand, within Pf6N2E2_106 at 151.798 kb on + strand, within Pf6N2E2_106 at 151.823 kb on - strand, within Pf6N2E2_106 at 151.825 kb on + strand, within Pf6N2E2_106 at 151.826 kb on - strand, within Pf6N2E2_106 at 151.826 kb on - strand, within Pf6N2E2_106 at 151.984 kb on + strand, within Pf6N2E2_106 at 151.984 kb on + strand, within Pf6N2E2_106 at 151.985 kb on - strand, within Pf6N2E2_106 at 151.985 kb on - strand, within Pf6N2E2_106 at 152.117 kb on - strand, within Pf6N2E2_106
Per-strain Table
Position Strand Gene LocusTag Fraction tungstate 0.05 M remove 149,914 + Pf6N2E2_104 0.25 +0.5 149,914 + Pf6N2E2_104 0.25 -1.1 149,914 + Pf6N2E2_104 0.25 +0.3 149,915 - Pf6N2E2_104 0.25 +1.0 149,915 - Pf6N2E2_104 0.25 +0.8 149,915 - Pf6N2E2_104 0.25 +0.4 149,942 + Pf6N2E2_104 0.27 +0.2 149,943 - Pf6N2E2_104 0.27 -1.3 149,943 - Pf6N2E2_104 0.27 +1.1 150,223 + Pf6N2E2_104 0.53 +0.6 150,224 - Pf6N2E2_104 0.53 +1.0 150,280 + Pf6N2E2_104 0.58 +0.1 150,281 - Pf6N2E2_104 0.58 -1.8 150,281 - Pf6N2E2_104 0.58 +1.5 150,532 + Pf6N2E2_104 0.81 +1.6 150,533 - Pf6N2E2_104 0.81 -0.7 150,583 + Pf6N2E2_104 0.85 +0.6 150,583 + Pf6N2E2_104 0.85 +0.1 150,584 - Pf6N2E2_104 0.85 -0.8 150,584 - Pf6N2E2_104 0.85 +0.7 150,627 - Pf6N2E2_104 0.89 +0.7 150,667 + -2.3 150,667 + -0.4 150,667 + -0.7 150,701 - -0.2 150,712 + -1.5 150,712 + -0.5 150,879 + Pf6N2E2_105 0.16 -0.8 151,167 + Pf6N2E2_105 0.49 -1.5 151,167 + Pf6N2E2_105 0.49 +0.5 151,168 - Pf6N2E2_105 0.49 -3.2 151,168 - Pf6N2E2_105 0.49 +0.2 151,173 + Pf6N2E2_105 0.49 -0.4 151,173 + Pf6N2E2_105 0.49 -0.8 151,174 - Pf6N2E2_105 0.50 +0.6 151,174 - Pf6N2E2_105 0.50 -1.0 151,174 - Pf6N2E2_105 0.50 +0.2 151,258 - Pf6N2E2_105 0.59 -0.3 151,287 + Pf6N2E2_105 0.63 +0.3 151,288 - Pf6N2E2_105 0.63 -0.3 151,288 - Pf6N2E2_105 0.63 -0.3 151,290 + Pf6N2E2_105 0.63 -0.1 151,290 + +0.9 151,290 + Pf6N2E2_105 0.63 -0.6 151,291 - Pf6N2E2_105 0.63 +0.6 151,413 + Pf6N2E2_105 0.77 -0.1 151,414 - Pf6N2E2_105 0.77 -1.5 151,540 - +1.1 151,540 - +0.4 151,540 - -0.5 151,540 - +0.8 151,575 + -0.3 151,575 + +0.8 151,654 + +0.5 151,655 - -1.8 151,655 - +0.9 151,655 - +0.2 151,681 + +0.4 151,695 + +0.1 151,695 + +0.2 151,696 - +0.0 151,768 + Pf6N2E2_106 0.18 -1.1 151,768 + Pf6N2E2_106 0.18 -0.6 151,768 + Pf6N2E2_106 0.18 -0.1 151,769 - Pf6N2E2_106 0.18 -0.3 151,798 + Pf6N2E2_106 0.22 -0.3 151,823 - Pf6N2E2_106 0.25 -0.8 151,825 + Pf6N2E2_106 0.25 +0.3 151,826 - Pf6N2E2_106 0.26 -0.4 151,826 - Pf6N2E2_106 0.26 -1.4 151,984 + Pf6N2E2_106 0.45 +0.6 151,984 + Pf6N2E2_106 0.45 -0.3 151,985 - Pf6N2E2_106 0.45 -1.2 151,985 - Pf6N2E2_106 0.45 +0.4 152,117 - Pf6N2E2_106 0.62 +0.2
Or see this region's nucleotide sequence