Experiment: L-Alanine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25280 and BWI76_RS25285 overlap by 4 nucleotides BWI76_RS25285 and BWI76_RS25290 are separated by 53 nucleotides BWI76_RS25290 and BWI76_RS25295 overlap by 4 nucleotides BWI76_RS25295 and BWI76_RS25300 are separated by 234 nucleotides
BWI76_RS25280: BWI76_RS25280 - RNase adaptor protein RapZ, at 5,254,190 to 5,255,044
_RS25280
BWI76_RS25285: BWI76_RS25285 - HPr family phosphocarrier protein, at 5,255,041 to 5,255,313
_RS25285
BWI76_RS25290: BWI76_RS25290 - monofunctional biosynthetic peptidoglycan transglycosylase, at 5,255,367 to 5,256,092
_RS25290
BWI76_RS25295: BWI76_RS25295 - glutamine amidotransferase, at 5,256,089 to 5,256,742
_RS25295
BWI76_RS25300: BWI76_RS25300 - hybrid sensor histidine kinase/response regulator, at 5,256,977 to 5,259,316
_RS25300
Position (kb) 5255
5256
5257 Strain fitness (log2 ratio) -2
-1
0
1 at 5254.555 kb on + strand, within BWI76_RS25280 at 5255.053 kb on - strand at 5255.069 kb on + strand, within BWI76_RS25285 at 5255.356 kb on - strand at 5255.396 kb on - strand at 5255.680 kb on - strand, within BWI76_RS25290 at 5255.899 kb on - strand, within BWI76_RS25290 at 5255.989 kb on + strand, within BWI76_RS25290 at 5255.989 kb on + strand, within BWI76_RS25290 at 5255.994 kb on + strand, within BWI76_RS25290 at 5255.997 kb on - strand, within BWI76_RS25290 at 5256.013 kb on + strand, within BWI76_RS25290 at 5256.021 kb on - strand at 5256.347 kb on + strand, within BWI76_RS25295 at 5256.468 kb on + strand, within BWI76_RS25295 at 5256.521 kb on - strand, within BWI76_RS25295 at 5256.556 kb on + strand, within BWI76_RS25295 at 5256.724 kb on - strand at 5256.729 kb on - strand at 5256.745 kb on + strand at 5256.960 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Alanine (N) remove 5,254,555 + BWI76_RS25280 0.43 -1.4 5,255,053 - -0.3 5,255,069 + BWI76_RS25285 0.10 -0.3 5,255,356 - +0.8 5,255,396 - -0.2 5,255,680 - BWI76_RS25290 0.43 -1.0 5,255,899 - BWI76_RS25290 0.73 -0.1 5,255,989 + BWI76_RS25290 0.86 -1.1 5,255,989 + BWI76_RS25290 0.86 +0.8 5,255,994 + BWI76_RS25290 0.86 -1.3 5,255,997 - BWI76_RS25290 0.87 +0.3 5,256,013 + BWI76_RS25290 0.89 -2.2 5,256,021 - +1.0 5,256,347 + BWI76_RS25295 0.39 +0.2 5,256,468 + BWI76_RS25295 0.58 -0.5 5,256,521 - BWI76_RS25295 0.66 +0.1 5,256,556 + BWI76_RS25295 0.71 -0.2 5,256,724 - -2.2 5,256,729 - -0.8 5,256,745 + +0.3 5,256,960 + +0.2
Or see this region's nucleotide sequence