Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS02450

Experiment: L-Alanine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS02435 and BWI76_RS02440 are separated by 140 nucleotidesBWI76_RS02440 and BWI76_RS02445 are separated by 10 nucleotidesBWI76_RS02445 and BWI76_RS02450 are separated by 10 nucleotidesBWI76_RS02450 and BWI76_RS02455 overlap by 8 nucleotides BWI76_RS02435: BWI76_RS02435 - outer membrane lipoprotein Blc, at 499,493 to 500,023 _RS02435 BWI76_RS02440: BWI76_RS02440 - fumarate reductase subunit D, at 500,164 to 500,523 _RS02440 BWI76_RS02445: BWI76_RS02445 - fumarate reductase subunit C, at 500,534 to 500,929 _RS02445 BWI76_RS02450: BWI76_RS02450 - succinate dehydrogenase/fumarate reductase iron-sulfur subunit, at 500,940 to 501,674 _RS02450 BWI76_RS02455: BWI76_RS02455 - fumarate reductase flavoprotein subunit, at 501,667 to 503,457 _RS02455 Position (kb) 500 501 502Strain fitness (log2 ratio) -1 0 1at 499.960 kb on + strand, within BWI76_RS02435at 499.960 kb on + strand, within BWI76_RS02435at 499.960 kb on + strand, within BWI76_RS02435at 500.052 kb on + strandat 500.299 kb on - strand, within BWI76_RS02440at 500.304 kb on + strand, within BWI76_RS02440at 500.463 kb on - strand, within BWI76_RS02440at 500.543 kb on - strandat 500.543 kb on - strandat 500.586 kb on + strand, within BWI76_RS02445at 500.762 kb on + strand, within BWI76_RS02445at 500.848 kb on + strand, within BWI76_RS02445at 500.850 kb on + strand, within BWI76_RS02445at 500.911 kb on - strandat 500.948 kb on + strandat 500.956 kb on - strandat 500.994 kb on - strandat 501.287 kb on + strand, within BWI76_RS02450at 501.420 kb on - strand, within BWI76_RS02450at 501.475 kb on - strand, within BWI76_RS02450at 501.505 kb on + strand, within BWI76_RS02450at 501.552 kb on - strand, within BWI76_RS02450at 501.601 kb on + strandat 501.601 kb on + strandat 501.688 kb on + strandat 501.874 kb on - strand, within BWI76_RS02455at 501.941 kb on + strand, within BWI76_RS02455at 501.941 kb on + strand, within BWI76_RS02455at 501.949 kb on - strand, within BWI76_RS02455at 501.949 kb on - strand, within BWI76_RS02455at 501.964 kb on - strand, within BWI76_RS02455at 501.974 kb on - strand, within BWI76_RS02455at 502.086 kb on + strand, within BWI76_RS02455at 502.122 kb on - strand, within BWI76_RS02455at 502.305 kb on - strand, within BWI76_RS02455at 502.381 kb on + strand, within BWI76_RS02455at 502.439 kb on + strand, within BWI76_RS02455at 502.483 kb on - strand, within BWI76_RS02455at 502.493 kb on - strand, within BWI76_RS02455at 502.529 kb on - strand, within BWI76_RS02455at 502.552 kb on + strand, within BWI76_RS02455at 502.553 kb on + strand, within BWI76_RS02455at 502.577 kb on + strand, within BWI76_RS02455at 502.607 kb on - strand, within BWI76_RS02455

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Alanine (N)
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499,960 + BWI76_RS02435 0.88 -0.4
499,960 + BWI76_RS02435 0.88 -0.3
499,960 + BWI76_RS02435 0.88 +0.0
500,052 + -0.3
500,299 - BWI76_RS02440 0.38 -0.1
500,304 + BWI76_RS02440 0.39 -1.5
500,463 - BWI76_RS02440 0.83 -0.0
500,543 - -1.0
500,543 - +0.0
500,586 + BWI76_RS02445 0.13 -0.6
500,762 + BWI76_RS02445 0.58 -0.7
500,848 + BWI76_RS02445 0.79 -0.1
500,850 + BWI76_RS02445 0.80 -0.5
500,911 - -0.3
500,948 + +0.4
500,956 - -0.5
500,994 - +0.0
501,287 + BWI76_RS02450 0.47 +0.3
501,420 - BWI76_RS02450 0.65 +0.4
501,475 - BWI76_RS02450 0.73 +0.3
501,505 + BWI76_RS02450 0.77 +0.2
501,552 - BWI76_RS02450 0.83 -1.2
501,601 + +0.0
501,601 + +0.7
501,688 + +0.2
501,874 - BWI76_RS02455 0.12 -0.2
501,941 + BWI76_RS02455 0.15 -0.0
501,941 + BWI76_RS02455 0.15 +0.1
501,949 - BWI76_RS02455 0.16 +0.0
501,949 - BWI76_RS02455 0.16 +0.1
501,964 - BWI76_RS02455 0.17 -0.3
501,974 - BWI76_RS02455 0.17 -0.6
502,086 + BWI76_RS02455 0.23 +0.3
502,122 - BWI76_RS02455 0.25 -0.2
502,305 - BWI76_RS02455 0.36 +0.3
502,381 + BWI76_RS02455 0.40 +0.5
502,439 + BWI76_RS02455 0.43 -0.3
502,483 - BWI76_RS02455 0.46 -0.7
502,493 - BWI76_RS02455 0.46 -0.2
502,529 - BWI76_RS02455 0.48 +0.2
502,552 + BWI76_RS02455 0.49 -0.2
502,553 + BWI76_RS02455 0.49 -0.0
502,577 + BWI76_RS02455 0.51 +0.3
502,607 - BWI76_RS02455 0.52 -0.2

Or see this region's nucleotide sequence