Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1897

Experiment: Fluorophosphate 0.125 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1895 and Pf6N2E2_1896 are separated by 212 nucleotidesPf6N2E2_1896 and Pf6N2E2_1897 are separated by 79 nucleotidesPf6N2E2_1897 and Pf6N2E2_1898 overlap by 4 nucleotides Pf6N2E2_1895 - PQQ-dependent oxidoreductase, gdhB family, at 2,245,833 to 2,246,951 _1895 Pf6N2E2_1896 - hypothetical protein, at 2,247,164 to 2,247,286 _1896 Pf6N2E2_1897 - Cytochrome B561, at 2,247,366 to 2,247,944 _1897 Pf6N2E2_1898 - Catalase (EC 1.11.1.6), at 2,247,941 to 2,249,023 _1898 Position (kb) 2247 2248Strain fitness (log2 ratio) -2 -1 0 1at 2246.444 kb on - strand, within Pf6N2E2_1895at 2246.606 kb on + strand, within Pf6N2E2_1895at 2246.606 kb on + strand, within Pf6N2E2_1895at 2246.648 kb on - strand, within Pf6N2E2_1895at 2246.992 kb on + strandat 2247.005 kb on - strandat 2247.005 kb on - strandat 2247.014 kb on + strandat 2247.014 kb on + strandat 2247.028 kb on - strandat 2247.151 kb on + strandat 2247.152 kb on - strandat 2247.204 kb on - strand, within Pf6N2E2_1896at 2247.204 kb on - strand, within Pf6N2E2_1896at 2247.204 kb on - strand, within Pf6N2E2_1896at 2247.289 kb on + strandat 2247.290 kb on - strandat 2247.290 kb on - strandat 2247.305 kb on + strandat 2247.524 kb on + strand, within Pf6N2E2_1897at 2247.524 kb on + strand, within Pf6N2E2_1897at 2247.524 kb on + strand, within Pf6N2E2_1897at 2247.524 kb on + strand, within Pf6N2E2_1897at 2247.525 kb on - strand, within Pf6N2E2_1897at 2247.664 kb on + strand, within Pf6N2E2_1897at 2247.664 kb on + strand, within Pf6N2E2_1897at 2247.664 kb on + strand, within Pf6N2E2_1897at 2247.665 kb on - strand, within Pf6N2E2_1897at 2247.665 kb on - strand, within Pf6N2E2_1897at 2247.665 kb on - strand, within Pf6N2E2_1897at 2247.824 kb on - strand, within Pf6N2E2_1897at 2247.824 kb on - strand, within Pf6N2E2_1897at 2247.994 kb on - strandat 2248.024 kb on - strandat 2248.252 kb on + strand, within Pf6N2E2_1898at 2248.253 kb on - strand, within Pf6N2E2_1898at 2248.352 kb on + strand, within Pf6N2E2_1898at 2248.352 kb on + strand, within Pf6N2E2_1898at 2248.352 kb on + strand, within Pf6N2E2_1898at 2248.435 kb on - strand, within Pf6N2E2_1898at 2248.449 kb on + strand, within Pf6N2E2_1898at 2248.572 kb on + strand, within Pf6N2E2_1898at 2248.741 kb on - strand, within Pf6N2E2_1898at 2248.741 kb on - strand, within Pf6N2E2_1898at 2248.819 kb on + strand, within Pf6N2E2_1898at 2248.820 kb on - strand, within Pf6N2E2_1898at 2248.930 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Fluorophosphate 0.125 M
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2,246,444 - Pf6N2E2_1895 0.55 -0.6
2,246,606 + Pf6N2E2_1895 0.69 -0.7
2,246,606 + Pf6N2E2_1895 0.69 -0.6
2,246,648 - Pf6N2E2_1895 0.73 -0.4
2,246,992 + +0.5
2,247,005 - +0.6
2,247,005 - +0.7
2,247,014 + +0.8
2,247,014 + +1.0
2,247,028 - -1.3
2,247,151 + +0.2
2,247,152 - +1.4
2,247,204 - Pf6N2E2_1896 0.33 -2.6
2,247,204 - Pf6N2E2_1896 0.33 +0.1
2,247,204 - Pf6N2E2_1896 0.33 -0.5
2,247,289 + -0.0
2,247,290 - +0.9
2,247,290 - +0.0
2,247,305 + +1.0
2,247,524 + Pf6N2E2_1897 0.27 -1.4
2,247,524 + Pf6N2E2_1897 0.27 -0.6
2,247,524 + Pf6N2E2_1897 0.27 -1.2
2,247,524 + Pf6N2E2_1897 0.27 -1.0
2,247,525 - Pf6N2E2_1897 0.27 +0.1
2,247,664 + Pf6N2E2_1897 0.51 +0.2
2,247,664 + Pf6N2E2_1897 0.51 +0.1
2,247,664 + Pf6N2E2_1897 0.51 -1.7
2,247,665 - Pf6N2E2_1897 0.52 -1.2
2,247,665 - Pf6N2E2_1897 0.52 -0.0
2,247,665 - Pf6N2E2_1897 0.52 -0.4
2,247,824 - Pf6N2E2_1897 0.79 -0.4
2,247,824 - Pf6N2E2_1897 0.79 -1.1
2,247,994 - +1.0
2,248,024 - -1.1
2,248,252 + Pf6N2E2_1898 0.29 -0.2
2,248,253 - Pf6N2E2_1898 0.29 +0.6
2,248,352 + Pf6N2E2_1898 0.38 -0.1
2,248,352 + Pf6N2E2_1898 0.38 -0.1
2,248,352 + Pf6N2E2_1898 0.38 -1.7
2,248,435 - Pf6N2E2_1898 0.46 -0.1
2,248,449 + Pf6N2E2_1898 0.47 -0.6
2,248,572 + Pf6N2E2_1898 0.58 -2.4
2,248,741 - Pf6N2E2_1898 0.74 -0.7
2,248,741 - Pf6N2E2_1898 0.74 -0.4
2,248,819 + Pf6N2E2_1898 0.81 -0.1
2,248,820 - Pf6N2E2_1898 0.81 -1.1
2,248,930 - -0.1

Or see this region's nucleotide sequence