Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS03190

Experiment: Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPFLU_RS03185 and PFLU_RS03190 are separated by 19 nucleotidesPFLU_RS03190 and PFLU_RS03195 are separated by 342 nucleotidesPFLU_RS03195 and PFLU_RS03200 are separated by 58 nucleotides PFLU_RS03185: PFLU_RS03185 - type II and III secretion system protein family protein, at 736,343 to 737,560 _RS03185 PFLU_RS03190: PFLU_RS03190 - Flp pilus assembly protein CpaB, at 737,580 to 738,521 _RS03190 PFLU_RS03195: PFLU_RS03195 - Flp family type IVb pilin, at 738,864 to 739,055 _RS03195 PFLU_RS03200: PFLU_RS03200 - response regulator, at 739,114 to 739,530 _RS03200 Position (kb) 737 738 739Strain fitness (log2 ratio) -2 -1 0 1 2 3at 736.657 kb on + strand, within PFLU_RS03185at 736.657 kb on + strand, within PFLU_RS03185at 736.657 kb on + strand, within PFLU_RS03185at 736.658 kb on - strand, within PFLU_RS03185at 736.722 kb on + strand, within PFLU_RS03185at 736.722 kb on + strand, within PFLU_RS03185at 736.722 kb on + strand, within PFLU_RS03185at 736.818 kb on + strand, within PFLU_RS03185at 736.818 kb on + strand, within PFLU_RS03185at 736.818 kb on + strand, within PFLU_RS03185at 736.818 kb on + strand, within PFLU_RS03185at 736.818 kb on + strand, within PFLU_RS03185at 736.818 kb on + strand, within PFLU_RS03185at 736.819 kb on - strand, within PFLU_RS03185at 736.819 kb on - strand, within PFLU_RS03185at 736.936 kb on - strand, within PFLU_RS03185at 736.936 kb on - strand, within PFLU_RS03185at 737.093 kb on + strand, within PFLU_RS03185at 737.094 kb on - strand, within PFLU_RS03185at 737.279 kb on + strand, within PFLU_RS03185at 737.280 kb on - strand, within PFLU_RS03185at 737.280 kb on - strand, within PFLU_RS03185at 737.359 kb on + strand, within PFLU_RS03185at 737.547 kb on + strandat 737.704 kb on + strand, within PFLU_RS03190at 737.704 kb on + strand, within PFLU_RS03190at 737.704 kb on + strand, within PFLU_RS03190at 737.761 kb on + strand, within PFLU_RS03190at 737.793 kb on + strand, within PFLU_RS03190at 737.794 kb on - strand, within PFLU_RS03190at 737.873 kb on + strand, within PFLU_RS03190at 737.873 kb on + strand, within PFLU_RS03190at 737.874 kb on - strand, within PFLU_RS03190at 737.874 kb on - strand, within PFLU_RS03190at 738.029 kb on + strand, within PFLU_RS03190at 738.030 kb on - strand, within PFLU_RS03190at 738.030 kb on - strand, within PFLU_RS03190at 738.030 kb on - strand, within PFLU_RS03190at 738.030 kb on - strand, within PFLU_RS03190at 738.067 kb on + strand, within PFLU_RS03190at 738.067 kb on + strand, within PFLU_RS03190at 738.067 kb on + strand, within PFLU_RS03190at 738.067 kb on + strand, within PFLU_RS03190at 738.447 kb on + strandat 738.447 kb on + strandat 738.559 kb on + strandat 738.603 kb on - strandat 738.614 kb on + strandat 738.624 kb on + strandat 738.624 kb on + strandat 738.625 kb on - strandat 738.625 kb on - strandat 738.674 kb on + strandat 738.675 kb on - strandat 738.675 kb on - strandat 738.675 kb on - strandat 738.702 kb on + strandat 738.702 kb on + strandat 738.736 kb on + strandat 738.739 kb on + strandat 738.739 kb on + strandat 738.739 kb on + strandat 738.739 kb on + strandat 738.740 kb on - strandat 738.740 kb on - strandat 738.740 kb on - strandat 738.761 kb on + strandat 738.761 kb on + strandat 738.761 kb on + strandat 738.761 kb on + strandat 738.762 kb on - strandat 738.797 kb on - strandat 738.797 kb on - strandat 738.821 kb on + strandat 738.822 kb on - strandat 738.822 kb on - strandat 738.822 kb on - strandat 738.839 kb on + strandat 738.840 kb on - strandat 738.840 kb on - strandat 738.850 kb on + strandat 738.863 kb on + strandat 738.863 kb on + strandat 738.863 kb on + strandat 738.863 kb on + strandat 738.863 kb on + strandat 738.863 kb on + strandat 738.864 kb on - strandat 738.864 kb on - strandat 738.864 kb on - strandat 738.864 kb on - strandat 738.864 kb on - strandat 738.864 kb on - strandat 738.864 kb on - strandat 738.864 kb on - strandat 738.864 kb on - strandat 738.891 kb on + strand, within PFLU_RS03195at 738.891 kb on + strand, within PFLU_RS03195at 738.892 kb on - strand, within PFLU_RS03195at 738.907 kb on + strand, within PFLU_RS03195at 738.907 kb on + strand, within PFLU_RS03195at 738.907 kb on + strand, within PFLU_RS03195at 738.908 kb on - strand, within PFLU_RS03195at 738.908 kb on - strand, within PFLU_RS03195at 738.908 kb on - strand, within PFLU_RS03195at 738.946 kb on - strand, within PFLU_RS03195at 738.958 kb on + strand, within PFLU_RS03195at 739.007 kb on + strand, within PFLU_RS03195at 739.007 kb on + strand, within PFLU_RS03195at 739.008 kb on - strand, within PFLU_RS03195at 739.008 kb on - strand, within PFLU_RS03195at 739.008 kb on - strand, within PFLU_RS03195at 739.008 kb on - strand, within PFLU_RS03195at 739.056 kb on + strandat 739.056 kb on + strandat 739.108 kb on - strandat 739.108 kb on - strandat 739.222 kb on - strand, within PFLU_RS03200at 739.406 kb on + strand, within PFLU_RS03200at 739.406 kb on + strand, within PFLU_RS03200at 739.406 kb on + strand, within PFLU_RS03200at 739.406 kb on + strand, within PFLU_RS03200at 739.406 kb on + strand, within PFLU_RS03200at 739.407 kb on - strand, within PFLU_RS03200at 739.407 kb on - strand, within PFLU_RS03200at 739.407 kb on - strand, within PFLU_RS03200at 739.407 kb on - strand, within PFLU_RS03200at 739.407 kb on - strand, within PFLU_RS03200at 739.407 kb on - strand, within PFLU_RS03200at 739.407 kb on - strand, within PFLU_RS03200at 739.456 kb on + strand, within PFLU_RS03200at 739.456 kb on + strand, within PFLU_RS03200at 739.456 kb on + strand, within PFLU_RS03200at 739.456 kb on + strand, within PFLU_RS03200at 739.457 kb on - strand, within PFLU_RS03200at 739.457 kb on - strand, within PFLU_RS03200at 739.457 kb on - strand, within PFLU_RS03200

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Per-strain Table

Position Strand Gene LocusTag Fraction Soil=PNNL_Prosser_PlotA_B_20191220; Sterility=sterile; Collection=outgrowth_in_LB_2hrs_then_extra_outgrowth_in_LB; SoilTreatment=coldshock; SoilTreatmentTime=72_hrs
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736,657 + PFLU_RS03185 0.26 +1.5
736,657 + PFLU_RS03185 0.26 +1.5
736,657 + PFLU_RS03185 0.26 -0.1
736,658 - PFLU_RS03185 0.26 +1.7
736,722 + PFLU_RS03185 0.31 +0.4
736,722 + PFLU_RS03185 0.31 +0.7
736,722 + PFLU_RS03185 0.31 +2.3
736,818 + PFLU_RS03185 0.39 +1.4
736,818 + PFLU_RS03185 0.39 +0.0
736,818 + PFLU_RS03185 0.39 +1.5
736,818 + PFLU_RS03185 0.39 +1.3
736,818 + PFLU_RS03185 0.39 +1.6
736,818 + PFLU_RS03185 0.39 +1.1
736,819 - PFLU_RS03185 0.39 +1.2
736,819 - PFLU_RS03185 0.39 +1.8
736,936 - PFLU_RS03185 0.49 +1.3
736,936 - PFLU_RS03185 0.49 +1.7
737,093 + PFLU_RS03185 0.62 -2.1
737,094 - PFLU_RS03185 0.62 +1.5
737,279 + PFLU_RS03185 0.77 +1.0
737,280 - PFLU_RS03185 0.77 +0.9
737,280 - PFLU_RS03185 0.77 +3.0
737,359 + PFLU_RS03185 0.83 +1.9
737,547 + +0.5
737,704 + PFLU_RS03190 0.13 +2.6
737,704 + PFLU_RS03190 0.13 +0.9
737,704 + PFLU_RS03190 0.13 +1.3
737,761 + PFLU_RS03190 0.19 +2.5
737,793 + PFLU_RS03190 0.23 +0.7
737,794 - PFLU_RS03190 0.23 +2.1
737,873 + PFLU_RS03190 0.31 -0.4
737,873 + PFLU_RS03190 0.31 +1.2
737,874 - PFLU_RS03190 0.31 +1.0
737,874 - PFLU_RS03190 0.31 +1.8
738,029 + PFLU_RS03190 0.48 +1.4
738,030 - PFLU_RS03190 0.48 +1.9
738,030 - PFLU_RS03190 0.48 +1.0
738,030 - PFLU_RS03190 0.48 +1.9
738,030 - PFLU_RS03190 0.48 +1.0
738,067 + PFLU_RS03190 0.52 +1.3
738,067 + PFLU_RS03190 0.52 +2.5
738,067 + PFLU_RS03190 0.52 +2.3
738,067 + PFLU_RS03190 0.52 +2.5
738,447 + +1.5
738,447 + +2.4
738,559 + +2.0
738,603 - -0.6
738,614 + +1.9
738,624 + +1.3
738,624 + +2.3
738,625 - +0.7
738,625 - -0.3
738,674 + -1.3
738,675 - +0.4
738,675 - +1.6
738,675 - +0.9
738,702 + +0.3
738,702 + -1.2
738,736 + +0.6
738,739 + +1.2
738,739 + +1.3
738,739 + -0.7
738,739 + +1.6
738,740 - -0.0
738,740 - +0.3
738,740 - +1.1
738,761 + +1.3
738,761 + +1.0
738,761 + +0.4
738,761 + +1.8
738,762 - +0.9
738,797 - +0.5
738,797 - +1.4
738,821 + +0.4
738,822 - +0.7
738,822 - +1.3
738,822 - -0.0
738,839 + +0.8
738,840 - +0.5
738,840 - +0.9
738,850 + -0.8
738,863 + +1.1
738,863 + +1.2
738,863 + +1.5
738,863 + +0.6
738,863 + +1.5
738,863 + -0.3
738,864 - +0.7
738,864 - +2.6
738,864 - +0.5
738,864 - +0.3
738,864 - +1.0
738,864 - +0.9
738,864 - +1.6
738,864 - +0.8
738,864 - +0.7
738,891 + PFLU_RS03195 0.14 +2.2
738,891 + PFLU_RS03195 0.14 +1.1
738,892 - PFLU_RS03195 0.15 -0.4
738,907 + PFLU_RS03195 0.22 +2.0
738,907 + PFLU_RS03195 0.22 +1.3
738,907 + PFLU_RS03195 0.22 +2.2
738,908 - PFLU_RS03195 0.23 +1.2
738,908 - PFLU_RS03195 0.23 +1.1
738,908 - PFLU_RS03195 0.23 +0.1
738,946 - PFLU_RS03195 0.43 +1.7
738,958 + PFLU_RS03195 0.49 +1.8
739,007 + PFLU_RS03195 0.74 +2.1
739,007 + PFLU_RS03195 0.74 +1.2
739,008 - PFLU_RS03195 0.75 +1.5
739,008 - PFLU_RS03195 0.75 -0.2
739,008 - PFLU_RS03195 0.75 +1.3
739,008 - PFLU_RS03195 0.75 +0.3
739,056 + -0.6
739,056 + +1.2
739,108 - -0.5
739,108 - +1.0
739,222 - PFLU_RS03200 0.26 +0.2
739,406 + PFLU_RS03200 0.70 -1.9
739,406 + PFLU_RS03200 0.70 -1.7
739,406 + PFLU_RS03200 0.70 -2.3
739,406 + PFLU_RS03200 0.70 +3.5
739,406 + PFLU_RS03200 0.70 +0.2
739,407 - PFLU_RS03200 0.70 +1.3
739,407 - PFLU_RS03200 0.70 +0.6
739,407 - PFLU_RS03200 0.70 +0.6
739,407 - PFLU_RS03200 0.70 -0.5
739,407 - PFLU_RS03200 0.70 +0.1
739,407 - PFLU_RS03200 0.70 -0.1
739,407 - PFLU_RS03200 0.70 +1.9
739,456 + PFLU_RS03200 0.82 +1.7
739,456 + PFLU_RS03200 0.82 -1.9
739,456 + PFLU_RS03200 0.82 +0.5
739,456 + PFLU_RS03200 0.82 +0.6
739,457 - PFLU_RS03200 0.82 -1.0
739,457 - PFLU_RS03200 0.82 -0.5
739,457 - PFLU_RS03200 0.82 +1.2

Or see this region's nucleotide sequence