Experiment: L-Proline (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02330 and BWI76_RS02335 are separated by 25 nucleotides BWI76_RS02335 and BWI76_RS02340 are separated by 154 nucleotides BWI76_RS02340 and BWI76_RS02345 are separated by 15 nucleotides
BWI76_RS02330: BWI76_RS02330 - aspartate ammonia-lyase, at 479,414 to 480,850
_RS02330
BWI76_RS02335: BWI76_RS02335 - hypothetical protein, at 480,876 to 481,034
_RS02335
BWI76_RS02340: BWI76_RS02340 - membrane protein FxsA, at 481,189 to 481,665
_RS02340
BWI76_RS02345: BWI76_RS02345 - L-methionine/branched-chain amino acid transporter, at 481,681 to 482,940
_RS02345
Position (kb) 481
482 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 480.325 kb on + strand, within BWI76_RS02330 at 480.370 kb on + strand, within BWI76_RS02330 at 480.436 kb on - strand, within BWI76_RS02330 at 480.471 kb on + strand, within BWI76_RS02330 at 480.498 kb on - strand, within BWI76_RS02330 at 480.498 kb on - strand, within BWI76_RS02330 at 480.537 kb on + strand, within BWI76_RS02330 at 480.564 kb on - strand, within BWI76_RS02330 at 480.567 kb on + strand, within BWI76_RS02330 at 480.604 kb on - strand, within BWI76_RS02330 at 480.689 kb on - strand, within BWI76_RS02330 at 480.689 kb on - strand, within BWI76_RS02330 at 480.752 kb on + strand at 480.799 kb on + strand at 480.870 kb on - strand at 480.947 kb on + strand, within BWI76_RS02335 at 480.959 kb on + strand, within BWI76_RS02335 at 481.137 kb on + strand at 481.172 kb on + strand at 481.258 kb on + strand, within BWI76_RS02340 at 481.259 kb on + strand, within BWI76_RS02340 at 481.259 kb on + strand, within BWI76_RS02340 at 481.259 kb on + strand, within BWI76_RS02340 at 481.267 kb on - strand, within BWI76_RS02340 at 481.279 kb on - strand, within BWI76_RS02340 at 481.317 kb on - strand, within BWI76_RS02340 at 481.365 kb on + strand, within BWI76_RS02340 at 481.611 kb on + strand, within BWI76_RS02340 at 481.694 kb on + strand at 481.737 kb on + strand at 481.818 kb on - strand, within BWI76_RS02345 at 481.867 kb on + strand, within BWI76_RS02345 at 481.967 kb on - strand, within BWI76_RS02345 at 481.969 kb on + strand, within BWI76_RS02345 at 481.977 kb on - strand, within BWI76_RS02345 at 481.977 kb on - strand, within BWI76_RS02345 at 482.036 kb on + strand, within BWI76_RS02345 at 482.036 kb on + strand, within BWI76_RS02345 at 482.041 kb on + strand, within BWI76_RS02345 at 482.054 kb on + strand, within BWI76_RS02345 at 482.057 kb on - strand, within BWI76_RS02345 at 482.062 kb on - strand, within BWI76_RS02345 at 482.082 kb on - strand, within BWI76_RS02345 at 482.098 kb on + strand, within BWI76_RS02345 at 482.150 kb on + strand, within BWI76_RS02345 at 482.237 kb on - strand, within BWI76_RS02345 at 482.427 kb on + strand, within BWI76_RS02345 at 482.512 kb on + strand, within BWI76_RS02345 at 482.610 kb on - strand, within BWI76_RS02345 at 482.626 kb on + strand, within BWI76_RS02345 at 482.634 kb on - strand, within BWI76_RS02345 at 482.634 kb on - strand, within BWI76_RS02345
Per-strain Table
Position Strand Gene LocusTag Fraction L-Proline (N) remove 480,325 + BWI76_RS02330 0.63 -3.8 480,370 + BWI76_RS02330 0.67 -3.6 480,436 - BWI76_RS02330 0.71 -1.5 480,471 + BWI76_RS02330 0.74 -0.8 480,498 - BWI76_RS02330 0.75 -4.0 480,498 - BWI76_RS02330 0.75 -2.2 480,537 + BWI76_RS02330 0.78 -1.5 480,564 - BWI76_RS02330 0.80 -2.5 480,567 + BWI76_RS02330 0.80 -2.6 480,604 - BWI76_RS02330 0.83 -1.9 480,689 - BWI76_RS02330 0.89 -2.2 480,689 - BWI76_RS02330 0.89 -2.5 480,752 + -0.4 480,799 + -2.2 480,870 - +0.7 480,947 + BWI76_RS02335 0.45 -2.2 480,959 + BWI76_RS02335 0.52 +0.8 481,137 + -0.7 481,172 + +1.0 481,258 + BWI76_RS02340 0.14 -0.0 481,259 + BWI76_RS02340 0.15 +0.3 481,259 + BWI76_RS02340 0.15 +0.7 481,259 + BWI76_RS02340 0.15 -0.3 481,267 - BWI76_RS02340 0.16 +0.1 481,279 - BWI76_RS02340 0.19 +0.1 481,317 - BWI76_RS02340 0.27 +0.2 481,365 + BWI76_RS02340 0.37 +0.0 481,611 + BWI76_RS02340 0.88 -0.2 481,694 + -1.9 481,737 + -0.3 481,818 - BWI76_RS02345 0.11 -0.3 481,867 + BWI76_RS02345 0.15 -0.4 481,967 - BWI76_RS02345 0.23 +1.1 481,969 + BWI76_RS02345 0.23 -0.3 481,977 - BWI76_RS02345 0.23 +0.7 481,977 - BWI76_RS02345 0.23 -1.0 482,036 + BWI76_RS02345 0.28 -3.6 482,036 + BWI76_RS02345 0.28 -1.6 482,041 + BWI76_RS02345 0.29 -0.8 482,054 + BWI76_RS02345 0.30 +0.2 482,057 - BWI76_RS02345 0.30 -1.0 482,062 - BWI76_RS02345 0.30 -2.2 482,082 - BWI76_RS02345 0.32 -0.3 482,098 + BWI76_RS02345 0.33 -0.0 482,150 + BWI76_RS02345 0.37 -0.8 482,237 - BWI76_RS02345 0.44 +0.8 482,427 + BWI76_RS02345 0.59 -0.4 482,512 + BWI76_RS02345 0.66 +0.7 482,610 - BWI76_RS02345 0.74 -1.1 482,626 + BWI76_RS02345 0.75 +0.3 482,634 - BWI76_RS02345 0.76 +0.4 482,634 - BWI76_RS02345 0.76 -0.9
Or see this region's nucleotide sequence