Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_85 and Pf6N2E2_86 are separated by 2 nucleotides Pf6N2E2_86 and Pf6N2E2_87 are separated by 570 nucleotides Pf6N2E2_87 and Pf6N2E2_88 are separated by 175 nucleotides
Pf6N2E2_85 - FIG045511: hypothetical antitoxin (to FIG022160: hypothetical toxin), at 129,986 to 130,294
_85
Pf6N2E2_86 - FIG022160: hypothetical toxin, at 130,297 to 130,467
_86
Pf6N2E2_87 - hypothetical protein, at 131,038 to 131,397
_87
Pf6N2E2_88 - hypothetical protein, at 131,573 to 131,746
_88
Position (kb) 131
132 Strain fitness (log2 ratio) -2
-1
0
1
2 at 130.061 kb on + strand, within Pf6N2E2_85 at 130.062 kb on - strand, within Pf6N2E2_85 at 130.104 kb on + strand, within Pf6N2E2_85 at 130.105 kb on - strand, within Pf6N2E2_85 at 130.105 kb on - strand, within Pf6N2E2_85 at 130.105 kb on - strand, within Pf6N2E2_85 at 130.105 kb on - strand, within Pf6N2E2_85 at 130.171 kb on - strand, within Pf6N2E2_85 at 130.227 kb on + strand, within Pf6N2E2_85 at 130.228 kb on - strand, within Pf6N2E2_85 at 130.228 kb on - strand, within Pf6N2E2_85 at 130.228 kb on - strand, within Pf6N2E2_85 at 130.228 kb on - strand, within Pf6N2E2_85 at 130.228 kb on - strand, within Pf6N2E2_85 at 130.256 kb on + strand, within Pf6N2E2_85 at 130.403 kb on + strand, within Pf6N2E2_86 at 130.403 kb on + strand, within Pf6N2E2_86 at 130.404 kb on - strand, within Pf6N2E2_86 at 130.423 kb on + strand, within Pf6N2E2_86 at 130.423 kb on + strand, within Pf6N2E2_86 at 130.424 kb on - strand, within Pf6N2E2_86 at 130.424 kb on - strand, within Pf6N2E2_86 at 130.446 kb on + strand, within Pf6N2E2_86 at 130.446 kb on + strand, within Pf6N2E2_86 at 130.447 kb on - strand, within Pf6N2E2_86 at 130.587 kb on + strand at 130.587 kb on + strand at 130.587 kb on + strand at 130.588 kb on - strand at 130.613 kb on - strand at 130.743 kb on + strand at 130.743 kb on + strand at 130.744 kb on - strand at 130.754 kb on + strand at 130.754 kb on + strand at 130.754 kb on + strand at 130.755 kb on - strand at 130.755 kb on - strand at 130.755 kb on - strand at 130.764 kb on - strand at 130.812 kb on + strand at 130.812 kb on + strand at 130.812 kb on + strand at 130.812 kb on + strand at 130.813 kb on - strand at 130.875 kb on + strand at 130.892 kb on + strand at 130.892 kb on + strand at 131.004 kb on + strand at 131.097 kb on + strand, within Pf6N2E2_87 at 131.242 kb on + strand, within Pf6N2E2_87 at 131.243 kb on - strand, within Pf6N2E2_87 at 131.284 kb on + strand, within Pf6N2E2_87 at 131.284 kb on + strand, within Pf6N2E2_87 at 131.284 kb on + strand, within Pf6N2E2_87 at 131.285 kb on - strand, within Pf6N2E2_87 at 131.285 kb on - strand, within Pf6N2E2_87 at 131.285 kb on - strand, within Pf6N2E2_87 at 131.285 kb on - strand, within Pf6N2E2_87 at 131.395 kb on + strand at 131.618 kb on + strand, within Pf6N2E2_88 at 131.687 kb on + strand, within Pf6N2E2_88 at 131.739 kb on - strand at 131.739 kb on - strand at 131.742 kb on + strand at 131.742 kb on + strand at 131.742 kb on + strand at 131.743 kb on - strand at 131.743 kb on - strand at 131.743 kb on - strand at 131.743 kb on - strand at 131.743 kb on - strand at 131.743 kb on - strand at 131.804 kb on + strand at 131.805 kb on - strand at 132.092 kb on - strand at 132.161 kb on + strand at 132.239 kb on - strand at 132.239 kb on - strand at 132.261 kb on + strand at 132.262 kb on - strand at 132.284 kb on + strand at 132.284 kb on + strand at 132.338 kb on + strand at 132.392 kb on + strand at 132.393 kb on - strand at 132.393 kb on - strand at 132.397 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 130,061 + Pf6N2E2_85 0.24 -0.1 130,062 - Pf6N2E2_85 0.25 +0.9 130,104 + Pf6N2E2_85 0.38 +0.8 130,105 - Pf6N2E2_85 0.39 +0.5 130,105 - Pf6N2E2_85 0.39 +0.8 130,105 - Pf6N2E2_85 0.39 +1.2 130,105 - Pf6N2E2_85 0.39 -1.0 130,171 - Pf6N2E2_85 0.60 -0.5 130,227 + Pf6N2E2_85 0.78 +0.7 130,228 - Pf6N2E2_85 0.78 +1.4 130,228 - Pf6N2E2_85 0.78 -0.8 130,228 - Pf6N2E2_85 0.78 -1.5 130,228 - Pf6N2E2_85 0.78 +0.5 130,228 - Pf6N2E2_85 0.78 +0.9 130,256 + Pf6N2E2_85 0.87 -0.3 130,403 + Pf6N2E2_86 0.62 -0.7 130,403 + Pf6N2E2_86 0.62 -1.7 130,404 - Pf6N2E2_86 0.63 -0.7 130,423 + Pf6N2E2_86 0.74 +0.9 130,423 + Pf6N2E2_86 0.74 -1.0 130,424 - Pf6N2E2_86 0.74 -0.9 130,424 - Pf6N2E2_86 0.74 +0.7 130,446 + Pf6N2E2_86 0.87 -1.0 130,446 + Pf6N2E2_86 0.87 +1.1 130,447 - Pf6N2E2_86 0.88 +0.1 130,587 + +0.6 130,587 + -0.9 130,587 + +0.9 130,588 - +2.1 130,613 - +1.7 130,743 + -0.0 130,743 + +1.2 130,744 - +0.9 130,754 + +1.4 130,754 + +0.7 130,754 + -0.1 130,755 - -0.8 130,755 - -0.5 130,755 - -0.3 130,764 - +0.5 130,812 + +0.6 130,812 + +0.5 130,812 + -2.2 130,812 + +0.4 130,813 - -1.8 130,875 + +1.5 130,892 + +0.8 130,892 + +0.1 131,004 + -0.3 131,097 + Pf6N2E2_87 0.16 -1.3 131,242 + Pf6N2E2_87 0.57 -0.7 131,243 - Pf6N2E2_87 0.57 -1.4 131,284 + Pf6N2E2_87 0.68 -0.3 131,284 + Pf6N2E2_87 0.68 -1.3 131,284 + Pf6N2E2_87 0.68 +0.6 131,285 - Pf6N2E2_87 0.69 -1.1 131,285 - Pf6N2E2_87 0.69 -0.7 131,285 - Pf6N2E2_87 0.69 -0.2 131,285 - Pf6N2E2_87 0.69 +0.1 131,395 + +0.5 131,618 + Pf6N2E2_88 0.26 +0.2 131,687 + Pf6N2E2_88 0.66 -1.0 131,739 - +1.4 131,739 - +0.2 131,742 + +0.5 131,742 + -0.4 131,742 + -0.5 131,743 - +1.1 131,743 - -0.4 131,743 - +0.1 131,743 - -0.1 131,743 - +0.5 131,743 - +0.4 131,804 + -1.1 131,805 - -0.7 132,092 - +0.7 132,161 + -2.3 132,239 - -0.1 132,239 - -0.8 132,261 + +0.1 132,262 - +1.1 132,284 + +0.4 132,284 + +0.2 132,338 + -0.1 132,392 + +0.7 132,393 - +0.4 132,393 - -2.5 132,397 - +0.3
Or see this region's nucleotide sequence