Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_224

Experiment: LB no stress 48 hours

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_223 and Pf6N2E2_224 are separated by 242 nucleotidesPf6N2E2_224 and Pf6N2E2_225 are separated by 88 nucleotides Pf6N2E2_223 - RND efflux system, outer membrane lipoprotein CmeC, at 283,578 to 284,975 _223 Pf6N2E2_224 - transcription factor nitR, at 285,218 to 286,216 _224 Pf6N2E2_225 - Putative benzaldehyde dehydrogenase oxidoreductase protein (EC 1.2.1.28), at 286,305 to 287,783 _225 Position (kb) 285 286 287Strain fitness (log2 ratio) -2 -1 0 1 2at 284.249 kb on + strand, within Pf6N2E2_223at 284.367 kb on + strand, within Pf6N2E2_223at 284.691 kb on + strand, within Pf6N2E2_223at 284.692 kb on - strand, within Pf6N2E2_223at 284.692 kb on - strand, within Pf6N2E2_223at 284.850 kb on + strandat 284.883 kb on + strandat 284.884 kb on - strandat 284.946 kb on + strandat 284.946 kb on + strandat 284.946 kb on + strandat 284.947 kb on - strandat 284.947 kb on - strandat 284.974 kb on - strandat 284.974 kb on - strandat 284.974 kb on - strandat 284.994 kb on + strandat 284.995 kb on - strandat 285.189 kb on - strandat 285.423 kb on + strand, within Pf6N2E2_224at 285.423 kb on + strand, within Pf6N2E2_224at 285.423 kb on + strand, within Pf6N2E2_224at 285.424 kb on - strand, within Pf6N2E2_224at 285.424 kb on - strand, within Pf6N2E2_224at 285.697 kb on - strand, within Pf6N2E2_224at 285.748 kb on + strand, within Pf6N2E2_224at 285.952 kb on + strand, within Pf6N2E2_224at 286.109 kb on + strand, within Pf6N2E2_224at 286.109 kb on + strand, within Pf6N2E2_224at 286.110 kb on - strand, within Pf6N2E2_224at 286.110 kb on - strand, within Pf6N2E2_224at 286.110 kb on - strand, within Pf6N2E2_224at 286.110 kb on - strand, within Pf6N2E2_224at 286.110 kb on - strand, within Pf6N2E2_224at 286.111 kb on + strand, within Pf6N2E2_224at 286.111 kb on + strand, within Pf6N2E2_224at 286.112 kb on - strand, within Pf6N2E2_224at 286.112 kb on - strand, within Pf6N2E2_224at 286.113 kb on + strand, within Pf6N2E2_224at 286.114 kb on - strand, within Pf6N2E2_224at 286.205 kb on + strandat 286.227 kb on + strandat 286.228 kb on - strandat 286.231 kb on + strandat 286.232 kb on - strandat 286.232 kb on - strandat 286.234 kb on + strandat 286.235 kb on - strandat 286.376 kb on - strandat 286.376 kb on - strandat 286.461 kb on - strand, within Pf6N2E2_225at 286.651 kb on - strand, within Pf6N2E2_225at 286.651 kb on - strand, within Pf6N2E2_225at 286.864 kb on + strand, within Pf6N2E2_225at 286.864 kb on + strand, within Pf6N2E2_225at 286.865 kb on - strand, within Pf6N2E2_225

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Per-strain Table

Position Strand Gene LocusTag Fraction LB no stress 48 hours
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284,249 + Pf6N2E2_223 0.48 +0.8
284,367 + Pf6N2E2_223 0.56 -1.4
284,691 + Pf6N2E2_223 0.80 -0.2
284,692 - Pf6N2E2_223 0.80 +0.5
284,692 - Pf6N2E2_223 0.80 -0.3
284,850 + +0.0
284,883 + -0.3
284,884 - -0.8
284,946 + +1.2
284,946 + -0.9
284,946 + -0.1
284,947 - +0.5
284,947 - +0.2
284,974 - +0.1
284,974 - +0.1
284,974 - -0.0
284,994 + -0.5
284,995 - +1.3
285,189 - +0.1
285,423 + Pf6N2E2_224 0.21 -0.5
285,423 + Pf6N2E2_224 0.21 +0.3
285,423 + Pf6N2E2_224 0.21 -1.1
285,424 - Pf6N2E2_224 0.21 -0.8
285,424 - Pf6N2E2_224 0.21 +1.5
285,697 - Pf6N2E2_224 0.48 +0.6
285,748 + Pf6N2E2_224 0.53 +0.2
285,952 + Pf6N2E2_224 0.73 -0.6
286,109 + Pf6N2E2_224 0.89 +1.0
286,109 + Pf6N2E2_224 0.89 -0.5
286,110 - Pf6N2E2_224 0.89 -0.8
286,110 - Pf6N2E2_224 0.89 -0.1
286,110 - Pf6N2E2_224 0.89 +1.3
286,110 - Pf6N2E2_224 0.89 +2.0
286,110 - Pf6N2E2_224 0.89 +1.6
286,111 + Pf6N2E2_224 0.89 +0.3
286,111 + Pf6N2E2_224 0.89 +1.3
286,112 - Pf6N2E2_224 0.89 +0.5
286,112 - Pf6N2E2_224 0.89 +0.5
286,113 + Pf6N2E2_224 0.90 -0.0
286,114 - Pf6N2E2_224 0.90 +1.0
286,205 + +0.9
286,227 + -0.4
286,228 - +0.8
286,231 + -0.2
286,232 - -0.8
286,232 - +0.7
286,234 + +0.4
286,235 - -1.5
286,376 - +0.2
286,376 - -2.0
286,461 - Pf6N2E2_225 0.11 +0.5
286,651 - Pf6N2E2_225 0.23 -0.4
286,651 - Pf6N2E2_225 0.23 -1.7
286,864 + Pf6N2E2_225 0.38 +1.0
286,864 + Pf6N2E2_225 0.38 +0.6
286,865 - Pf6N2E2_225 0.38 -1.3

Or see this region's nucleotide sequence