Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_223 and Pf6N2E2_224 are separated by 242 nucleotides Pf6N2E2_224 and Pf6N2E2_225 are separated by 88 nucleotides
Pf6N2E2_223 - RND efflux system, outer membrane lipoprotein CmeC, at 283,578 to 284,975
_223
Pf6N2E2_224 - transcription factor nitR, at 285,218 to 286,216
_224
Pf6N2E2_225 - Putative benzaldehyde dehydrogenase oxidoreductase protein (EC 1.2.1.28), at 286,305 to 287,783
_225
Position (kb)
285
286
287 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 284.249 kb on + strand, within Pf6N2E2_223 at 284.367 kb on + strand, within Pf6N2E2_223 at 284.691 kb on + strand, within Pf6N2E2_223 at 284.692 kb on - strand, within Pf6N2E2_223 at 284.692 kb on - strand, within Pf6N2E2_223 at 284.850 kb on + strand at 284.883 kb on + strand at 284.884 kb on - strand at 284.946 kb on + strand at 284.946 kb on + strand at 284.946 kb on + strand at 284.947 kb on - strand at 284.947 kb on - strand at 284.974 kb on - strand at 284.974 kb on - strand at 284.974 kb on - strand at 284.994 kb on + strand at 284.995 kb on - strand at 285.189 kb on - strand at 285.423 kb on + strand, within Pf6N2E2_224 at 285.423 kb on + strand, within Pf6N2E2_224 at 285.423 kb on + strand, within Pf6N2E2_224 at 285.424 kb on - strand, within Pf6N2E2_224 at 285.424 kb on - strand, within Pf6N2E2_224 at 285.697 kb on - strand, within Pf6N2E2_224 at 285.748 kb on + strand, within Pf6N2E2_224 at 285.952 kb on + strand, within Pf6N2E2_224 at 286.109 kb on + strand, within Pf6N2E2_224 at 286.109 kb on + strand, within Pf6N2E2_224 at 286.110 kb on - strand, within Pf6N2E2_224 at 286.110 kb on - strand, within Pf6N2E2_224 at 286.110 kb on - strand, within Pf6N2E2_224 at 286.110 kb on - strand, within Pf6N2E2_224 at 286.110 kb on - strand, within Pf6N2E2_224 at 286.111 kb on + strand, within Pf6N2E2_224 at 286.111 kb on + strand, within Pf6N2E2_224 at 286.112 kb on - strand, within Pf6N2E2_224 at 286.112 kb on - strand, within Pf6N2E2_224 at 286.113 kb on + strand, within Pf6N2E2_224 at 286.114 kb on - strand, within Pf6N2E2_224 at 286.205 kb on + strand at 286.227 kb on + strand at 286.228 kb on - strand at 286.231 kb on + strand at 286.232 kb on - strand at 286.232 kb on - strand at 286.234 kb on + strand at 286.235 kb on - strand at 286.376 kb on - strand at 286.376 kb on - strand at 286.461 kb on - strand, within Pf6N2E2_225 at 286.651 kb on - strand, within Pf6N2E2_225 at 286.651 kb on - strand, within Pf6N2E2_225 at 286.864 kb on + strand, within Pf6N2E2_225 at 286.864 kb on + strand, within Pf6N2E2_225 at 286.865 kb on - strand, within Pf6N2E2_225
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 284,249 + Pf6N2E2_223 0.48 +0.8 284,367 + Pf6N2E2_223 0.56 -1.4 284,691 + Pf6N2E2_223 0.80 -0.2 284,692 - Pf6N2E2_223 0.80 +0.5 284,692 - Pf6N2E2_223 0.80 -0.3 284,850 + +0.0 284,883 + -0.3 284,884 - -0.8 284,946 + +1.2 284,946 + -0.9 284,946 + -0.1 284,947 - +0.5 284,947 - +0.2 284,974 - +0.1 284,974 - +0.1 284,974 - -0.0 284,994 + -0.5 284,995 - +1.3 285,189 - +0.1 285,423 + Pf6N2E2_224 0.21 -0.5 285,423 + Pf6N2E2_224 0.21 +0.3 285,423 + Pf6N2E2_224 0.21 -1.1 285,424 - Pf6N2E2_224 0.21 -0.8 285,424 - Pf6N2E2_224 0.21 +1.5 285,697 - Pf6N2E2_224 0.48 +0.6 285,748 + Pf6N2E2_224 0.53 +0.2 285,952 + Pf6N2E2_224 0.73 -0.6 286,109 + Pf6N2E2_224 0.89 +1.0 286,109 + Pf6N2E2_224 0.89 -0.5 286,110 - Pf6N2E2_224 0.89 -0.8 286,110 - Pf6N2E2_224 0.89 -0.1 286,110 - Pf6N2E2_224 0.89 +1.3 286,110 - Pf6N2E2_224 0.89 +2.0 286,110 - Pf6N2E2_224 0.89 +1.6 286,111 + Pf6N2E2_224 0.89 +0.3 286,111 + Pf6N2E2_224 0.89 +1.3 286,112 - Pf6N2E2_224 0.89 +0.5 286,112 - Pf6N2E2_224 0.89 +0.5 286,113 + Pf6N2E2_224 0.90 -0.0 286,114 - Pf6N2E2_224 0.90 +1.0 286,205 + +0.9 286,227 + -0.4 286,228 - +0.8 286,231 + -0.2 286,232 - -0.8 286,232 - +0.7 286,234 + +0.4 286,235 - -1.5 286,376 - +0.2 286,376 - -2.0 286,461 - Pf6N2E2_225 0.11 +0.5 286,651 - Pf6N2E2_225 0.23 -0.4 286,651 - Pf6N2E2_225 0.23 -1.7 286,864 + Pf6N2E2_225 0.38 +1.0 286,864 + Pf6N2E2_225 0.38 +0.6 286,865 - Pf6N2E2_225 0.38 -1.3
Or see this region's nucleotide sequence