Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1480 and Pf6N2E2_1481 are separated by 437 nucleotides Pf6N2E2_1481 and Pf6N2E2_1482 are separated by 152 nucleotides
Pf6N2E2_1480 - SAM-dependent methyltransferases, at 1,729,161 to 1,729,868
_1480
Pf6N2E2_1481 - VgrG protein, at 1,730,306 to 1,731,634
_1481
Pf6N2E2_1482 - Probable periplasmic spermidine/putrescine-binding protein, at 1,731,787 to 1,732,890
_1482
Position (kb)
1730
1731
1732 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1729.385 kb on - strand, within Pf6N2E2_1480 at 1729.428 kb on + strand, within Pf6N2E2_1480 at 1729.549 kb on - strand, within Pf6N2E2_1480 at 1729.602 kb on + strand, within Pf6N2E2_1480 at 1729.602 kb on + strand, within Pf6N2E2_1480 at 1729.602 kb on + strand, within Pf6N2E2_1480 at 1729.756 kb on - strand, within Pf6N2E2_1480 at 1729.775 kb on - strand, within Pf6N2E2_1480 at 1729.775 kb on - strand, within Pf6N2E2_1480 at 1729.775 kb on - strand, within Pf6N2E2_1480 at 1729.790 kb on + strand, within Pf6N2E2_1480 at 1729.791 kb on - strand, within Pf6N2E2_1480 at 1729.903 kb on - strand at 1729.903 kb on - strand at 1729.955 kb on - strand at 1729.955 kb on - strand at 1729.955 kb on - strand at 1729.955 kb on - strand at 1729.981 kb on + strand at 1730.135 kb on - strand at 1730.141 kb on + strand at 1730.142 kb on - strand at 1730.142 kb on - strand at 1730.144 kb on + strand at 1730.144 kb on + strand at 1730.145 kb on - strand at 1730.145 kb on - strand at 1730.145 kb on - strand at 1730.151 kb on + strand at 1730.152 kb on - strand at 1730.244 kb on + strand at 1730.245 kb on - strand at 1730.262 kb on + strand at 1730.543 kb on + strand, within Pf6N2E2_1481 at 1730.544 kb on - strand, within Pf6N2E2_1481 at 1730.544 kb on - strand, within Pf6N2E2_1481 at 1730.544 kb on - strand, within Pf6N2E2_1481 at 1730.544 kb on - strand, within Pf6N2E2_1481 at 1730.697 kb on - strand, within Pf6N2E2_1481 at 1730.697 kb on - strand, within Pf6N2E2_1481 at 1730.697 kb on - strand, within Pf6N2E2_1481 at 1730.735 kb on + strand, within Pf6N2E2_1481 at 1730.858 kb on - strand, within Pf6N2E2_1481 at 1730.871 kb on - strand, within Pf6N2E2_1481 at 1730.897 kb on - strand, within Pf6N2E2_1481 at 1730.899 kb on - strand, within Pf6N2E2_1481 at 1730.949 kb on + strand, within Pf6N2E2_1481 at 1730.950 kb on - strand, within Pf6N2E2_1481 at 1730.950 kb on - strand, within Pf6N2E2_1481 at 1731.189 kb on + strand, within Pf6N2E2_1481 at 1731.201 kb on + strand, within Pf6N2E2_1481 at 1731.216 kb on + strand, within Pf6N2E2_1481 at 1731.310 kb on - strand, within Pf6N2E2_1481 at 1731.310 kb on - strand, within Pf6N2E2_1481 at 1731.340 kb on - strand, within Pf6N2E2_1481 at 1731.411 kb on + strand at 1731.528 kb on + strand at 1731.529 kb on - strand at 1731.650 kb on + strand at 1731.654 kb on + strand at 1731.655 kb on - strand at 1731.740 kb on - strand at 1731.772 kb on - strand at 1731.864 kb on + strand at 1731.865 kb on - strand at 1732.007 kb on + strand, within Pf6N2E2_1482 at 1732.188 kb on + strand, within Pf6N2E2_1482 at 1732.189 kb on - strand, within Pf6N2E2_1482 at 1732.241 kb on + strand, within Pf6N2E2_1482 at 1732.242 kb on - strand, within Pf6N2E2_1482 at 1732.280 kb on + strand, within Pf6N2E2_1482 at 1732.296 kb on + strand, within Pf6N2E2_1482 at 1732.297 kb on - strand, within Pf6N2E2_1482 at 1732.472 kb on + strand, within Pf6N2E2_1482 at 1732.495 kb on + strand, within Pf6N2E2_1482 at 1732.496 kb on - strand, within Pf6N2E2_1482 at 1732.500 kb on - strand, within Pf6N2E2_1482 at 1732.588 kb on - strand, within Pf6N2E2_1482
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 1,729,385 - Pf6N2E2_1480 0.32 -0.4 1,729,428 + Pf6N2E2_1480 0.38 +0.7 1,729,549 - Pf6N2E2_1480 0.55 +0.4 1,729,602 + Pf6N2E2_1480 0.62 -0.4 1,729,602 + Pf6N2E2_1480 0.62 -1.9 1,729,602 + Pf6N2E2_1480 0.62 +0.6 1,729,756 - Pf6N2E2_1480 0.84 +1.4 1,729,775 - Pf6N2E2_1480 0.87 +0.6 1,729,775 - Pf6N2E2_1480 0.87 -1.1 1,729,775 - Pf6N2E2_1480 0.87 -2.7 1,729,790 + Pf6N2E2_1480 0.89 +0.5 1,729,791 - Pf6N2E2_1480 0.89 -0.5 1,729,903 - +1.5 1,729,903 - -1.0 1,729,955 - +0.1 1,729,955 - -0.5 1,729,955 - -0.2 1,729,955 - -0.9 1,729,981 + -0.3 1,730,135 - -0.6 1,730,141 + -1.6 1,730,142 - -0.4 1,730,142 - +0.0 1,730,144 + -0.3 1,730,144 + +0.4 1,730,145 - +0.1 1,730,145 - -1.5 1,730,145 - -0.2 1,730,151 + -2.2 1,730,152 - +1.7 1,730,244 + -0.1 1,730,245 - +1.1 1,730,262 + +0.0 1,730,543 + Pf6N2E2_1481 0.18 -0.4 1,730,544 - Pf6N2E2_1481 0.18 -1.6 1,730,544 - Pf6N2E2_1481 0.18 +1.1 1,730,544 - Pf6N2E2_1481 0.18 -0.5 1,730,544 - Pf6N2E2_1481 0.18 -0.6 1,730,697 - Pf6N2E2_1481 0.29 -1.3 1,730,697 - Pf6N2E2_1481 0.29 -1.6 1,730,697 - Pf6N2E2_1481 0.29 +0.2 1,730,735 + Pf6N2E2_1481 0.32 +0.3 1,730,858 - Pf6N2E2_1481 0.42 -0.5 1,730,871 - Pf6N2E2_1481 0.43 +0.4 1,730,897 - Pf6N2E2_1481 0.44 -0.3 1,730,899 - Pf6N2E2_1481 0.45 -2.0 1,730,949 + Pf6N2E2_1481 0.48 -1.6 1,730,950 - Pf6N2E2_1481 0.48 +0.1 1,730,950 - Pf6N2E2_1481 0.48 -0.0 1,731,189 + Pf6N2E2_1481 0.66 +2.1 1,731,201 + Pf6N2E2_1481 0.67 -0.3 1,731,216 + Pf6N2E2_1481 0.68 -0.6 1,731,310 - Pf6N2E2_1481 0.76 +0.5 1,731,310 - Pf6N2E2_1481 0.76 -0.9 1,731,340 - Pf6N2E2_1481 0.78 -1.1 1,731,411 + +0.1 1,731,528 + -0.2 1,731,529 - -0.5 1,731,650 + +0.9 1,731,654 + -1.7 1,731,655 - -0.2 1,731,740 - +0.0 1,731,772 - +1.0 1,731,864 + -0.9 1,731,865 - +0.3 1,732,007 + Pf6N2E2_1482 0.20 +0.4 1,732,188 + Pf6N2E2_1482 0.36 -0.3 1,732,189 - Pf6N2E2_1482 0.36 +0.4 1,732,241 + Pf6N2E2_1482 0.41 -0.7 1,732,242 - Pf6N2E2_1482 0.41 +0.5 1,732,280 + Pf6N2E2_1482 0.45 +0.7 1,732,296 + Pf6N2E2_1482 0.46 +0.3 1,732,297 - Pf6N2E2_1482 0.46 +1.4 1,732,472 + Pf6N2E2_1482 0.62 +1.0 1,732,495 + Pf6N2E2_1482 0.64 -1.1 1,732,496 - Pf6N2E2_1482 0.64 +1.7 1,732,500 - Pf6N2E2_1482 0.65 -0.6 1,732,588 - Pf6N2E2_1482 0.73 -1.2
Or see this region's nucleotide sequence