Experiment: LB no stress 48 hours
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1207 and Pf6N2E2_1208 are separated by 60 nucleotides Pf6N2E2_1208 and Pf6N2E2_1209 are separated by 37 nucleotides Pf6N2E2_1209 and Pf6N2E2_1210 are separated by 345 nucleotides
Pf6N2E2_1207 - Epoxide hydrolase (EC 3.3.2.9), at 1,413,724 to 1,414,797
_1207
Pf6N2E2_1208 - Epoxide hydrolase (EC 3.3.2.9), at 1,414,858 to 1,416,228
_1208
Pf6N2E2_1209 - hypothetical protein, at 1,416,266 to 1,416,523
_1209
Pf6N2E2_1210 - putative aminotransferase, at 1,416,869 to 1,418,017
_1210
Position (kb)
1414
1415
1416
1417 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1413.953 kb on + strand, within Pf6N2E2_1207 at 1413.954 kb on - strand, within Pf6N2E2_1207 at 1413.992 kb on + strand, within Pf6N2E2_1207 at 1414.169 kb on + strand, within Pf6N2E2_1207 at 1414.517 kb on + strand, within Pf6N2E2_1207 at 1414.575 kb on - strand, within Pf6N2E2_1207 at 1414.642 kb on - strand, within Pf6N2E2_1207 at 1414.860 kb on - strand at 1414.897 kb on - strand at 1414.992 kb on - strand at 1414.992 kb on - strand at 1415.115 kb on + strand, within Pf6N2E2_1208 at 1415.115 kb on + strand, within Pf6N2E2_1208 at 1415.174 kb on + strand, within Pf6N2E2_1208 at 1415.262 kb on - strand, within Pf6N2E2_1208 at 1415.381 kb on + strand, within Pf6N2E2_1208 at 1415.561 kb on + strand, within Pf6N2E2_1208 at 1415.562 kb on - strand, within Pf6N2E2_1208 at 1415.562 kb on - strand, within Pf6N2E2_1208 at 1415.562 kb on - strand, within Pf6N2E2_1208 at 1415.562 kb on - strand, within Pf6N2E2_1208 at 1415.906 kb on + strand, within Pf6N2E2_1208 at 1415.921 kb on + strand, within Pf6N2E2_1208 at 1415.921 kb on + strand, within Pf6N2E2_1208 at 1415.921 kb on + strand at 1415.922 kb on - strand, within Pf6N2E2_1208 at 1415.938 kb on + strand, within Pf6N2E2_1208 at 1416.303 kb on + strand, within Pf6N2E2_1209 at 1416.304 kb on - strand, within Pf6N2E2_1209 at 1416.304 kb on - strand, within Pf6N2E2_1209 at 1416.339 kb on + strand, within Pf6N2E2_1209 at 1416.421 kb on - strand, within Pf6N2E2_1209 at 1416.426 kb on + strand, within Pf6N2E2_1209 at 1416.426 kb on + strand, within Pf6N2E2_1209 at 1416.426 kb on + strand, within Pf6N2E2_1209 at 1416.426 kb on + strand, within Pf6N2E2_1209 at 1416.426 kb on + strand, within Pf6N2E2_1209 at 1416.427 kb on - strand, within Pf6N2E2_1209 at 1416.427 kb on - strand, within Pf6N2E2_1209 at 1416.427 kb on - strand, within Pf6N2E2_1209 at 1416.529 kb on + strand at 1416.530 kb on - strand at 1416.530 kb on - strand at 1416.530 kb on - strand at 1416.662 kb on - strand at 1416.662 kb on - strand at 1416.672 kb on - strand at 1416.672 kb on - strand at 1416.672 kb on - strand at 1416.672 kb on - strand at 1416.779 kb on - strand at 1417.094 kb on + strand, within Pf6N2E2_1210
Per-strain Table
Position Strand Gene LocusTag Fraction LB no stress 48 hours remove 1,413,953 + Pf6N2E2_1207 0.21 +0.4 1,413,954 - Pf6N2E2_1207 0.21 +0.8 1,413,992 + Pf6N2E2_1207 0.25 -1.0 1,414,169 + Pf6N2E2_1207 0.41 -1.8 1,414,517 + Pf6N2E2_1207 0.74 -0.2 1,414,575 - Pf6N2E2_1207 0.79 +0.8 1,414,642 - Pf6N2E2_1207 0.85 +0.9 1,414,860 - -1.0 1,414,897 - -1.9 1,414,992 - -0.6 1,414,992 - +0.9 1,415,115 + Pf6N2E2_1208 0.19 +0.1 1,415,115 + Pf6N2E2_1208 0.19 -0.1 1,415,174 + Pf6N2E2_1208 0.23 -0.9 1,415,262 - Pf6N2E2_1208 0.29 -1.8 1,415,381 + Pf6N2E2_1208 0.38 +0.4 1,415,561 + Pf6N2E2_1208 0.51 +0.1 1,415,562 - Pf6N2E2_1208 0.51 -1.5 1,415,562 - Pf6N2E2_1208 0.51 +0.9 1,415,562 - Pf6N2E2_1208 0.51 +0.6 1,415,562 - Pf6N2E2_1208 0.51 -1.2 1,415,906 + Pf6N2E2_1208 0.76 +0.4 1,415,921 + Pf6N2E2_1208 0.78 -0.3 1,415,921 + Pf6N2E2_1208 0.78 +0.3 1,415,921 + +2.7 1,415,922 - Pf6N2E2_1208 0.78 -0.6 1,415,938 + Pf6N2E2_1208 0.79 -1.7 1,416,303 + Pf6N2E2_1209 0.14 +1.1 1,416,304 - Pf6N2E2_1209 0.15 -1.1 1,416,304 - Pf6N2E2_1209 0.15 -0.7 1,416,339 + Pf6N2E2_1209 0.28 +0.8 1,416,421 - Pf6N2E2_1209 0.60 +0.8 1,416,426 + Pf6N2E2_1209 0.62 +1.5 1,416,426 + Pf6N2E2_1209 0.62 +0.4 1,416,426 + Pf6N2E2_1209 0.62 -1.7 1,416,426 + Pf6N2E2_1209 0.62 +0.3 1,416,426 + Pf6N2E2_1209 0.62 +0.7 1,416,427 - Pf6N2E2_1209 0.62 +0.5 1,416,427 - Pf6N2E2_1209 0.62 -1.0 1,416,427 - Pf6N2E2_1209 0.62 -0.0 1,416,529 + +0.7 1,416,530 - +0.0 1,416,530 - -1.5 1,416,530 - +1.3 1,416,662 - +0.3 1,416,662 - +0.4 1,416,672 - +0.1 1,416,672 - -0.3 1,416,672 - +0.2 1,416,672 - -2.0 1,416,779 - -0.6 1,417,094 + Pf6N2E2_1210 0.20 -0.7
Or see this region's nucleotide sequence