Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS24875

Experiment: L-Cysteine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS24870 and BWI76_RS24875 are separated by 176 nucleotidesBWI76_RS24875 and BWI76_RS24880 are separated by 13 nucleotidesBWI76_RS24880 and BWI76_RS24885 are separated by 26 nucleotidesBWI76_RS24885 and BWI76_RS24890 are separated by 5 nucleotides BWI76_RS24870: BWI76_RS24870 - phosphocarrier, HPr family protein, at 5,183,033 to 5,183,848 _RS24870 BWI76_RS24875: BWI76_RS24875 - tagatose-1,6-bisphosphate aldolase, at 5,184,025 to 5,185,296 _RS24875 BWI76_RS24880: BWI76_RS24880 - PTS galactitol transporter subunit IIA, at 5,185,310 to 5,185,777 _RS24880 BWI76_RS24885: BWI76_RS24885 - PTS galactitol transporter subunit IIB, at 5,185,804 to 5,186,088 _RS24885 BWI76_RS24890: BWI76_RS24890 - PTS system galactitol-specific transporter subunit IIC, at 5,186,094 to 5,187,467 _RS24890 Position (kb) 5184 5185 5186Strain fitness (log2 ratio) -2 -1 0 1at 5183.051 kb on - strandat 5183.116 kb on + strand, within BWI76_RS24870at 5183.124 kb on - strand, within BWI76_RS24870at 5183.363 kb on + strand, within BWI76_RS24870at 5183.364 kb on + strand, within BWI76_RS24870at 5183.372 kb on - strand, within BWI76_RS24870at 5183.389 kb on + strand, within BWI76_RS24870at 5183.423 kb on + strand, within BWI76_RS24870at 5183.489 kb on + strand, within BWI76_RS24870at 5183.514 kb on + strand, within BWI76_RS24870at 5183.538 kb on + strand, within BWI76_RS24870at 5183.670 kb on + strand, within BWI76_RS24870at 5183.954 kb on + strandat 5183.962 kb on - strandat 5183.962 kb on - strandat 5183.962 kb on - strandat 5184.138 kb on + strandat 5184.150 kb on + strandat 5184.266 kb on + strand, within BWI76_RS24875at 5184.295 kb on - strand, within BWI76_RS24875at 5184.400 kb on + strand, within BWI76_RS24875at 5184.410 kb on + strand, within BWI76_RS24875at 5184.510 kb on - strand, within BWI76_RS24875at 5184.588 kb on - strand, within BWI76_RS24875at 5184.658 kb on + strand, within BWI76_RS24875at 5184.708 kb on - strand, within BWI76_RS24875at 5184.871 kb on + strand, within BWI76_RS24875at 5184.879 kb on - strand, within BWI76_RS24875at 5184.879 kb on - strand, within BWI76_RS24875at 5185.029 kb on - strand, within BWI76_RS24875at 5185.049 kb on + strand, within BWI76_RS24875at 5185.072 kb on + strand, within BWI76_RS24875at 5185.167 kb on + strand, within BWI76_RS24875at 5185.167 kb on + strand, within BWI76_RS24875at 5185.167 kb on + strand, within BWI76_RS24875at 5185.167 kb on + strand, within BWI76_RS24875at 5185.175 kb on - strandat 5185.175 kb on - strandat 5185.175 kb on - strandat 5185.175 kb on - strandat 5185.242 kb on + strandat 5185.267 kb on + strandat 5185.437 kb on - strand, within BWI76_RS24880at 5185.495 kb on + strand, within BWI76_RS24880at 5185.495 kb on - strand, within BWI76_RS24880at 5185.644 kb on - strand, within BWI76_RS24880at 5185.837 kb on - strand, within BWI76_RS24885at 5185.965 kb on - strand, within BWI76_RS24885at 5185.991 kb on + strand, within BWI76_RS24885at 5186.091 kb on + strandat 5186.200 kb on + strandat 5186.200 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Cysteine (N)
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5,183,051 - -0.7
5,183,116 + BWI76_RS24870 0.10 +0.8
5,183,124 - BWI76_RS24870 0.11 +0.8
5,183,363 + BWI76_RS24870 0.40 -0.5
5,183,364 + BWI76_RS24870 0.41 -0.4
5,183,372 - BWI76_RS24870 0.42 -0.0
5,183,389 + BWI76_RS24870 0.44 -0.8
5,183,423 + BWI76_RS24870 0.48 +1.1
5,183,489 + BWI76_RS24870 0.56 -0.9
5,183,514 + BWI76_RS24870 0.59 +0.5
5,183,538 + BWI76_RS24870 0.62 -0.1
5,183,670 + BWI76_RS24870 0.78 -0.6
5,183,954 + -0.8
5,183,962 - -1.8
5,183,962 - +1.4
5,183,962 - +0.7
5,184,138 + -0.2
5,184,150 + -0.7
5,184,266 + BWI76_RS24875 0.19 -0.1
5,184,295 - BWI76_RS24875 0.21 -0.7
5,184,400 + BWI76_RS24875 0.29 -1.1
5,184,410 + BWI76_RS24875 0.30 -0.0
5,184,510 - BWI76_RS24875 0.38 +0.0
5,184,588 - BWI76_RS24875 0.44 +1.0
5,184,658 + BWI76_RS24875 0.50 -0.4
5,184,708 - BWI76_RS24875 0.54 +0.4
5,184,871 + BWI76_RS24875 0.67 +0.6
5,184,879 - BWI76_RS24875 0.67 -0.0
5,184,879 - BWI76_RS24875 0.67 +1.0
5,185,029 - BWI76_RS24875 0.79 -1.7
5,185,049 + BWI76_RS24875 0.81 -1.0
5,185,072 + BWI76_RS24875 0.82 +1.6
5,185,167 + BWI76_RS24875 0.90 +1.7
5,185,167 + BWI76_RS24875 0.90 +1.0
5,185,167 + BWI76_RS24875 0.90 +0.3
5,185,167 + BWI76_RS24875 0.90 -1.4
5,185,175 - -1.4
5,185,175 - -0.6
5,185,175 - +0.1
5,185,175 - -0.1
5,185,242 + -0.4
5,185,267 + -1.1
5,185,437 - BWI76_RS24880 0.27 -0.8
5,185,495 + BWI76_RS24880 0.40 -0.7
5,185,495 - BWI76_RS24880 0.40 -0.7
5,185,644 - BWI76_RS24880 0.71 -0.8
5,185,837 - BWI76_RS24885 0.12 -1.1
5,185,965 - BWI76_RS24885 0.56 -1.0
5,185,991 + BWI76_RS24885 0.66 +0.6
5,186,091 + -0.4
5,186,200 + -0.6
5,186,200 + -0.3

Or see this region's nucleotide sequence