Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS09240

Experiment: L-Cysteine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS09220 and BWI76_RS09225 overlap by 37 nucleotidesBWI76_RS09225 and BWI76_RS09230 are separated by 67 nucleotidesBWI76_RS09230 and BWI76_RS09235 overlap by 1 nucleotidesBWI76_RS09235 and BWI76_RS09240 overlap by 4 nucleotidesBWI76_RS09240 and BWI76_RS09245 overlap by 4 nucleotides BWI76_RS09220: BWI76_RS09220 - hypothetical protein, at 1,944,068 to 1,944,268 _RS09220 BWI76_RS09225: BWI76_RS09225 - hypothetical protein, at 1,944,232 to 1,944,573 _RS09225 BWI76_RS09230: BWI76_RS09230 - hypothetical protein, at 1,944,641 to 1,944,877 _RS09230 BWI76_RS09235: BWI76_RS09235 - TraR/DksA family transcriptional regulator, at 1,944,877 to 1,945,104 _RS09235 BWI76_RS09240: BWI76_RS09240 - phosphoadenosine phosphosulfate reductase, at 1,945,101 to 1,946,111 _RS09240 BWI76_RS09245: BWI76_RS09245 - bacteriophage replication gene A protein, at 1,946,108 to 1,948,519 _RS09245 Position (kb) 1945 1946 1947Strain fitness (log2 ratio) -2 -1 0 1 2at 1944.131 kb on + strand, within BWI76_RS09220at 1944.154 kb on + strand, within BWI76_RS09220at 1944.155 kb on + strand, within BWI76_RS09220at 1944.178 kb on - strand, within BWI76_RS09220at 1944.198 kb on + strand, within BWI76_RS09220at 1944.206 kb on - strand, within BWI76_RS09220at 1944.221 kb on - strand, within BWI76_RS09220at 1944.246 kb on - strandat 1944.259 kb on + strandat 1944.267 kb on - strandat 1944.267 kb on - strandat 1944.269 kb on + strand, within BWI76_RS09225at 1944.269 kb on + strand, within BWI76_RS09225at 1944.269 kb on + strand, within BWI76_RS09225at 1944.274 kb on + strandat 1944.277 kb on - strand, within BWI76_RS09225at 1944.277 kb on - strand, within BWI76_RS09225at 1944.290 kb on + strand, within BWI76_RS09225at 1944.302 kb on - strand, within BWI76_RS09225at 1944.322 kb on - strand, within BWI76_RS09225at 1944.469 kb on - strand, within BWI76_RS09225at 1944.478 kb on - strand, within BWI76_RS09225at 1944.485 kb on - strand, within BWI76_RS09225at 1944.485 kb on - strand, within BWI76_RS09225at 1944.490 kb on - strand, within BWI76_RS09225at 1944.502 kb on - strand, within BWI76_RS09225at 1944.504 kb on + strand, within BWI76_RS09225at 1944.504 kb on + strand, within BWI76_RS09225at 1944.512 kb on - strand, within BWI76_RS09225at 1944.512 kb on - strand, within BWI76_RS09225at 1944.540 kb on - strandat 1944.804 kb on + strand, within BWI76_RS09230at 1944.812 kb on - strand, within BWI76_RS09230at 1944.852 kb on - strand, within BWI76_RS09230at 1944.893 kb on + strandat 1944.896 kb on + strandat 1945.182 kb on - strandat 1945.182 kb on - strandat 1945.218 kb on - strand, within BWI76_RS09240at 1945.331 kb on + strand, within BWI76_RS09240at 1945.361 kb on + strandat 1945.535 kb on + strand, within BWI76_RS09240at 1945.606 kb on + strand, within BWI76_RS09240at 1946.216 kb on + strandat 1946.235 kb on + strandat 1946.370 kb on + strand, within BWI76_RS09245at 1946.378 kb on - strand, within BWI76_RS09245at 1946.440 kb on + strand, within BWI76_RS09245at 1946.467 kb on - strand, within BWI76_RS09245at 1946.586 kb on + strand, within BWI76_RS09245at 1946.594 kb on - strand, within BWI76_RS09245at 1946.604 kb on - strand, within BWI76_RS09245at 1946.657 kb on + strand, within BWI76_RS09245at 1946.701 kb on + strand, within BWI76_RS09245at 1946.985 kb on + strand, within BWI76_RS09245at 1947.111 kb on + strand, within BWI76_RS09245

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Cysteine (N)
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1,944,131 + BWI76_RS09220 0.31 -1.1
1,944,154 + BWI76_RS09220 0.43 -0.3
1,944,155 + BWI76_RS09220 0.43 +0.2
1,944,178 - BWI76_RS09220 0.55 -0.1
1,944,198 + BWI76_RS09220 0.65 -0.6
1,944,206 - BWI76_RS09220 0.69 -0.1
1,944,221 - BWI76_RS09220 0.76 +0.7
1,944,246 - -0.1
1,944,259 + -1.8
1,944,267 - -0.6
1,944,267 - +2.3
1,944,269 + BWI76_RS09225 0.11 -0.4
1,944,269 + BWI76_RS09225 0.11 +1.9
1,944,269 + BWI76_RS09225 0.11 -0.7
1,944,274 + +2.0
1,944,277 - BWI76_RS09225 0.13 +0.9
1,944,277 - BWI76_RS09225 0.13 +0.4
1,944,290 + BWI76_RS09225 0.17 -0.8
1,944,302 - BWI76_RS09225 0.20 -1.2
1,944,322 - BWI76_RS09225 0.26 -0.5
1,944,469 - BWI76_RS09225 0.69 -0.2
1,944,478 - BWI76_RS09225 0.72 -0.5
1,944,485 - BWI76_RS09225 0.74 +0.7
1,944,485 - BWI76_RS09225 0.74 +1.4
1,944,490 - BWI76_RS09225 0.75 +0.2
1,944,502 - BWI76_RS09225 0.79 -1.2
1,944,504 + BWI76_RS09225 0.80 -0.5
1,944,504 + BWI76_RS09225 0.80 +0.7
1,944,512 - BWI76_RS09225 0.82 -0.0
1,944,512 - BWI76_RS09225 0.82 -2.3
1,944,540 - -0.6
1,944,804 + BWI76_RS09230 0.69 +0.6
1,944,812 - BWI76_RS09230 0.72 +0.6
1,944,852 - BWI76_RS09230 0.89 -0.6
1,944,893 + +0.2
1,944,896 + +0.5
1,945,182 - +0.2
1,945,182 - -0.2
1,945,218 - BWI76_RS09240 0.12 +0.0
1,945,331 + BWI76_RS09240 0.23 -0.2
1,945,361 + +0.0
1,945,535 + BWI76_RS09240 0.43 +0.9
1,945,606 + BWI76_RS09240 0.50 +1.4
1,946,216 + +0.7
1,946,235 + +2.3
1,946,370 + BWI76_RS09245 0.11 -1.5
1,946,378 - BWI76_RS09245 0.11 -0.6
1,946,440 + BWI76_RS09245 0.14 -1.0
1,946,467 - BWI76_RS09245 0.15 -0.2
1,946,586 + BWI76_RS09245 0.20 +0.9
1,946,594 - BWI76_RS09245 0.20 -0.3
1,946,604 - BWI76_RS09245 0.21 -1.8
1,946,657 + BWI76_RS09245 0.23 -2.0
1,946,701 + BWI76_RS09245 0.25 -2.6
1,946,985 + BWI76_RS09245 0.36 +0.4
1,947,111 + BWI76_RS09245 0.42 +0.9

Or see this region's nucleotide sequence