Experiment: PYE_noCalcium 30C survival rep2 12 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_02278 and CCNA_02279 overlap by 4 nucleotides CCNA_02279 and CCNA_02280 overlap by 4 nucleotides CCNA_02280 and CCNA_02281 are separated by 91 nucleotides
CCNA_02278: CCNA_02278 - hypothetical protein, at 2,429,076 to 2,429,684
_02278
CCNA_02279: CCNA_02279 - disulfide bond formation protein B, at 2,429,681 to 2,430,310
_02279
CCNA_02280: CCNA_02280 - 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase, at 2,430,307 to 2,430,867
_02280
CCNA_02281: CCNA_02281 - membrane alanine aminopeptidase, at 2,430,959 to 2,432,740
_02281
Position (kb)
2430
2431 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2429.309 kb on + strand, within CCNA_02278 at 2429.327 kb on + strand, within CCNA_02278 at 2429.345 kb on + strand, within CCNA_02278 at 2429.395 kb on + strand, within CCNA_02278 at 2429.411 kb on + strand, within CCNA_02278 at 2429.446 kb on - strand, within CCNA_02278 at 2429.491 kb on + strand, within CCNA_02278 at 2429.511 kb on + strand, within CCNA_02278 at 2429.538 kb on + strand, within CCNA_02278 at 2429.546 kb on - strand, within CCNA_02278 at 2429.564 kb on + strand, within CCNA_02278 at 2429.580 kb on + strand, within CCNA_02278 at 2429.594 kb on - strand, within CCNA_02278 at 2429.627 kb on - strand at 2429.661 kb on - strand at 2429.701 kb on - strand at 2429.718 kb on - strand at 2429.722 kb on - strand at 2429.740 kb on + strand at 2429.740 kb on + strand at 2429.740 kb on + strand at 2429.740 kb on + strand at 2429.740 kb on + strand at 2429.740 kb on + strand at 2429.740 kb on + strand at 2429.740 kb on + strand at 2429.747 kb on + strand, within CCNA_02279 at 2429.748 kb on - strand, within CCNA_02279 at 2429.797 kb on - strand, within CCNA_02279 at 2429.804 kb on + strand, within CCNA_02279 at 2429.824 kb on - strand, within CCNA_02279 at 2430.299 kb on - strand at 2430.869 kb on - strand at 2430.869 kb on - strand at 2430.935 kb on + strand at 2430.977 kb on - strand at 2431.041 kb on + strand at 2431.053 kb on + strand at 2431.063 kb on - strand at 2431.123 kb on + strand at 2431.187 kb on - strand, within CCNA_02281 at 2431.297 kb on + strand, within CCNA_02281 at 2431.297 kb on + strand, within CCNA_02281 at 2431.304 kb on + strand, within CCNA_02281 at 2431.310 kb on - strand, within CCNA_02281 at 2431.320 kb on + strand, within CCNA_02281 at 2431.320 kb on + strand, within CCNA_02281 at 2431.328 kb on - strand, within CCNA_02281 at 2431.388 kb on + strand, within CCNA_02281 at 2431.417 kb on - strand, within CCNA_02281 at 2431.430 kb on - strand, within CCNA_02281 at 2431.480 kb on - strand, within CCNA_02281 at 2431.495 kb on + strand, within CCNA_02281 at 2431.495 kb on + strand, within CCNA_02281 at 2431.503 kb on - strand, within CCNA_02281 at 2431.513 kb on - strand, within CCNA_02281 at 2431.546 kb on + strand, within CCNA_02281 at 2431.552 kb on + strand, within CCNA_02281 at 2431.554 kb on - strand, within CCNA_02281 at 2431.597 kb on + strand, within CCNA_02281 at 2431.597 kb on + strand, within CCNA_02281 at 2431.597 kb on - strand, within CCNA_02281 at 2431.634 kb on - strand, within CCNA_02281 at 2431.634 kb on - strand, within CCNA_02281 at 2431.648 kb on + strand, within CCNA_02281 at 2431.657 kb on - strand, within CCNA_02281 at 2431.657 kb on - strand, within CCNA_02281 at 2431.699 kb on - strand, within CCNA_02281 at 2431.771 kb on + strand, within CCNA_02281 at 2431.771 kb on + strand, within CCNA_02281 at 2431.772 kb on + strand, within CCNA_02281 at 2431.779 kb on - strand, within CCNA_02281 at 2431.830 kb on + strand, within CCNA_02281 at 2431.839 kb on - strand, within CCNA_02281 at 2431.850 kb on + strand, within CCNA_02281
Per-strain Table
Position Strand Gene LocusTag Fraction PYE_noCalcium 30C survival rep2 12 days remove 2,429,309 + CCNA_02278 0.38 -3.9 2,429,327 + CCNA_02278 0.41 -3.6 2,429,345 + CCNA_02278 0.44 -0.3 2,429,395 + CCNA_02278 0.52 -2.8 2,429,411 + CCNA_02278 0.55 -3.3 2,429,446 - CCNA_02278 0.61 +0.5 2,429,491 + CCNA_02278 0.68 -2.3 2,429,511 + CCNA_02278 0.71 -1.8 2,429,538 + CCNA_02278 0.76 -1.2 2,429,546 - CCNA_02278 0.77 -1.0 2,429,564 + CCNA_02278 0.80 +0.3 2,429,580 + CCNA_02278 0.83 -3.4 2,429,594 - CCNA_02278 0.85 -0.5 2,429,627 - -0.6 2,429,661 - +0.7 2,429,701 - +0.3 2,429,718 - +0.4 2,429,722 - -0.9 2,429,740 + -0.8 2,429,740 + -0.6 2,429,740 + -0.1 2,429,740 + -0.7 2,429,740 + +0.2 2,429,740 + -0.0 2,429,740 + -0.4 2,429,740 + +0.5 2,429,747 + CCNA_02279 0.10 +0.3 2,429,748 - CCNA_02279 0.11 -0.7 2,429,797 - CCNA_02279 0.18 -1.7 2,429,804 + CCNA_02279 0.20 +0.0 2,429,824 - CCNA_02279 0.23 -0.6 2,430,299 - +0.5 2,430,869 - +0.3 2,430,869 - -0.1 2,430,935 + -0.1 2,430,977 - +2.5 2,431,041 + -0.3 2,431,053 + +0.1 2,431,063 - -0.8 2,431,123 + -0.6 2,431,187 - CCNA_02281 0.13 +0.5 2,431,297 + CCNA_02281 0.19 +0.6 2,431,297 + CCNA_02281 0.19 -0.8 2,431,304 + CCNA_02281 0.19 -0.8 2,431,310 - CCNA_02281 0.20 -1.3 2,431,320 + CCNA_02281 0.20 -0.1 2,431,320 + CCNA_02281 0.20 -0.5 2,431,328 - CCNA_02281 0.21 +0.9 2,431,388 + CCNA_02281 0.24 -0.5 2,431,417 - CCNA_02281 0.26 +0.7 2,431,430 - CCNA_02281 0.26 -0.7 2,431,480 - CCNA_02281 0.29 -0.5 2,431,495 + CCNA_02281 0.30 -1.0 2,431,495 + CCNA_02281 0.30 +2.5 2,431,503 - CCNA_02281 0.31 +0.2 2,431,513 - CCNA_02281 0.31 -0.4 2,431,546 + CCNA_02281 0.33 -0.0 2,431,552 + CCNA_02281 0.33 +1.9 2,431,554 - CCNA_02281 0.33 -1.3 2,431,597 + CCNA_02281 0.36 -0.8 2,431,597 + CCNA_02281 0.36 -1.8 2,431,597 - CCNA_02281 0.36 -0.4 2,431,634 - CCNA_02281 0.38 -1.0 2,431,634 - CCNA_02281 0.38 -1.5 2,431,648 + CCNA_02281 0.39 -0.7 2,431,657 - CCNA_02281 0.39 -1.3 2,431,657 - CCNA_02281 0.39 -1.8 2,431,699 - CCNA_02281 0.42 +0.2 2,431,771 + CCNA_02281 0.46 -0.3 2,431,771 + CCNA_02281 0.46 -0.3 2,431,772 + CCNA_02281 0.46 +1.5 2,431,779 - CCNA_02281 0.46 +0.4 2,431,830 + CCNA_02281 0.49 -0.3 2,431,839 - CCNA_02281 0.49 -0.7 2,431,850 + CCNA_02281 0.50 +0.3
Or see this region's nucleotide sequence