Experiment: Gjerstad humics 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_297 and Pf6N2E2_298 are separated by 57 nucleotides Pf6N2E2_298 and Pf6N2E2_299 are separated by 103 nucleotides
Pf6N2E2_297 - Cell division protein FtsK, at 366,212 to 368,617
_297
Pf6N2E2_298 - Outer membrane lipoprotein carrier protein LolA, at 368,675 to 369,298
_298
Pf6N2E2_299 - FIG065221: Holliday junction DNA helicase, at 369,402 to 370,727
_299
Position (kb) 368
369
370 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 369.360 kb on - strand at 369.360 kb on - strand at 369.394 kb on - strand at 369.394 kb on - strand at 369.394 kb on - strand at 369.397 kb on + strand at 369.397 kb on + strand at 369.397 kb on + strand at 369.397 kb on + strand at 369.398 kb on - strand at 369.474 kb on + strand at 369.475 kb on - strand at 369.475 kb on - strand at 369.780 kb on + strand, within Pf6N2E2_299 at 369.780 kb on + strand, within Pf6N2E2_299 at 369.873 kb on + strand, within Pf6N2E2_299 at 369.873 kb on + strand, within Pf6N2E2_299 at 369.873 kb on + strand, within Pf6N2E2_299 at 369.873 kb on + strand, within Pf6N2E2_299 at 369.874 kb on - strand, within Pf6N2E2_299 at 369.874 kb on - strand, within Pf6N2E2_299 at 369.874 kb on - strand, within Pf6N2E2_299 at 369.874 kb on - strand, within Pf6N2E2_299 at 369.874 kb on - strand, within Pf6N2E2_299 at 369.874 kb on - strand, within Pf6N2E2_299 at 370.207 kb on - strand, within Pf6N2E2_299 at 370.248 kb on + strand, within Pf6N2E2_299 at 370.248 kb on + strand, within Pf6N2E2_299 at 370.249 kb on - strand, within Pf6N2E2_299
Per-strain Table
Position Strand Gene LocusTag Fraction Gjerstad humics 0.5 M remove 369,360 - -2.3 369,360 - -0.3 369,394 - -2.7 369,394 - -3.6 369,394 - -1.1 369,397 + -1.6 369,397 + -0.7 369,397 + -0.5 369,397 + -0.9 369,398 - -2.8 369,474 + +0.3 369,475 - -1.7 369,475 - +2.3 369,780 + Pf6N2E2_299 0.29 +0.3 369,780 + Pf6N2E2_299 0.29 +1.7 369,873 + Pf6N2E2_299 0.36 +0.0 369,873 + Pf6N2E2_299 0.36 +0.6 369,873 + Pf6N2E2_299 0.36 +0.9 369,873 + Pf6N2E2_299 0.36 +0.1 369,874 - Pf6N2E2_299 0.36 -0.5 369,874 - Pf6N2E2_299 0.36 -1.3 369,874 - Pf6N2E2_299 0.36 -2.7 369,874 - Pf6N2E2_299 0.36 -0.5 369,874 - Pf6N2E2_299 0.36 -1.0 369,874 - Pf6N2E2_299 0.36 -1.5 370,207 - Pf6N2E2_299 0.61 -1.5 370,248 + Pf6N2E2_299 0.64 -2.1 370,248 + Pf6N2E2_299 0.64 -0.7 370,249 - Pf6N2E2_299 0.64 -2.1
Or see this region's nucleotide sequence