Experiment: Gjerstad humics 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1465 and Pf6N2E2_1466 are separated by 429 nucleotides Pf6N2E2_1466 and Pf6N2E2_1467 are separated by 20 nucleotides Pf6N2E2_1467 and Pf6N2E2_1468 overlap by 4 nucleotides
Pf6N2E2_1465 - hypothetical protein, at 1,712,863 to 1,714,203
_1465
Pf6N2E2_1466 - NAD-dependent formate dehydrogenase delta subunit, at 1,714,633 to 1,714,830
_1466
Pf6N2E2_1467 - NAD-dependent formate dehydrogenase alpha subunit, at 1,714,851 to 1,717,727
_1467
Pf6N2E2_1468 - NAD-dependent formate dehydrogenase beta subunit, at 1,717,724 to 1,719,283
_1468
Position (kb)
1714
1715
1716
1717
1718 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1713.915 kb on - strand, within Pf6N2E2_1465 at 1713.976 kb on + strand, within Pf6N2E2_1465 at 1713.976 kb on + strand, within Pf6N2E2_1465 at 1713.976 kb on + strand, within Pf6N2E2_1465 at 1713.976 kb on + strand, within Pf6N2E2_1465 at 1714.000 kb on - strand, within Pf6N2E2_1465 at 1714.000 kb on - strand, within Pf6N2E2_1465 at 1714.074 kb on + strand at 1714.074 kb on + strand at 1714.074 kb on + strand at 1714.075 kb on - strand at 1714.075 kb on - strand at 1714.075 kb on - strand at 1714.075 kb on - strand at 1714.079 kb on + strand at 1714.079 kb on + strand at 1714.079 kb on + strand at 1714.080 kb on - strand at 1714.080 kb on - strand at 1714.080 kb on - strand at 1714.080 kb on - strand at 1714.094 kb on + strand at 1714.094 kb on + strand at 1714.094 kb on + strand at 1714.095 kb on - strand at 1714.448 kb on - strand at 1714.448 kb on - strand at 1714.459 kb on + strand at 1714.459 kb on + strand at 1714.459 kb on + strand at 1714.460 kb on - strand at 1714.460 kb on - strand at 1714.487 kb on - strand at 1714.611 kb on - strand at 1714.624 kb on + strand at 1714.624 kb on + strand at 1714.635 kb on - strand at 1714.785 kb on + strand, within Pf6N2E2_1466 at 1714.785 kb on + strand, within Pf6N2E2_1466 at 1714.785 kb on + strand, within Pf6N2E2_1466 at 1714.785 kb on + strand, within Pf6N2E2_1466 at 1714.785 kb on + strand, within Pf6N2E2_1466 at 1714.785 kb on + strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.786 kb on - strand, within Pf6N2E2_1466 at 1714.809 kb on - strand, within Pf6N2E2_1466 at 1714.809 kb on - strand, within Pf6N2E2_1466 at 1714.809 kb on - strand, within Pf6N2E2_1466 at 1714.834 kb on + strand at 1714.987 kb on + strand at 1714.987 kb on + strand at 1714.987 kb on + strand at 1714.987 kb on + strand at 1714.988 kb on - strand at 1714.988 kb on - strand at 1715.068 kb on + strand at 1715.069 kb on - strand at 1715.257 kb on + strand, within Pf6N2E2_1467 at 1715.258 kb on - strand, within Pf6N2E2_1467 at 1715.258 kb on - strand, within Pf6N2E2_1467 at 1715.361 kb on + strand, within Pf6N2E2_1467 at 1715.361 kb on + strand, within Pf6N2E2_1467 at 1715.518 kb on + strand, within Pf6N2E2_1467 at 1715.518 kb on + strand, within Pf6N2E2_1467 at 1715.519 kb on - strand, within Pf6N2E2_1467 at 1716.112 kb on + strand, within Pf6N2E2_1467 at 1716.112 kb on + strand, within Pf6N2E2_1467 at 1716.113 kb on - strand, within Pf6N2E2_1467 at 1716.113 kb on - strand, within Pf6N2E2_1467 at 1716.113 kb on - strand, within Pf6N2E2_1467 at 1716.115 kb on + strand, within Pf6N2E2_1467 at 1716.115 kb on + strand, within Pf6N2E2_1467 at 1716.116 kb on - strand, within Pf6N2E2_1467 at 1716.116 kb on - strand, within Pf6N2E2_1467 at 1716.116 kb on - strand, within Pf6N2E2_1467 at 1716.116 kb on - strand, within Pf6N2E2_1467 at 1716.116 kb on - strand, within Pf6N2E2_1467 at 1716.116 kb on - strand, within Pf6N2E2_1467 at 1716.142 kb on + strand, within Pf6N2E2_1467 at 1716.142 kb on + strand, within Pf6N2E2_1467 at 1716.143 kb on - strand, within Pf6N2E2_1467 at 1716.648 kb on + strand, within Pf6N2E2_1467 at 1716.667 kb on + strand, within Pf6N2E2_1467 at 1716.667 kb on + strand, within Pf6N2E2_1467 at 1716.668 kb on - strand, within Pf6N2E2_1467 at 1716.668 kb on - strand, within Pf6N2E2_1467 at 1716.725 kb on - strand, within Pf6N2E2_1467 at 1716.760 kb on + strand, within Pf6N2E2_1467 at 1716.979 kb on + strand, within Pf6N2E2_1467 at 1716.992 kb on + strand, within Pf6N2E2_1467 at 1717.393 kb on + strand, within Pf6N2E2_1467 at 1717.394 kb on - strand, within Pf6N2E2_1467 at 1717.394 kb on - strand, within Pf6N2E2_1467 at 1717.404 kb on + strand, within Pf6N2E2_1467 at 1717.405 kb on - strand, within Pf6N2E2_1467 at 1717.405 kb on - strand, within Pf6N2E2_1467 at 1717.405 kb on - strand, within Pf6N2E2_1467 at 1717.830 kb on + strand at 1717.831 kb on - strand at 1718.085 kb on + strand, within Pf6N2E2_1468 at 1718.311 kb on - strand, within Pf6N2E2_1468 at 1718.482 kb on + strand, within Pf6N2E2_1468 at 1718.482 kb on + strand, within Pf6N2E2_1468 at 1718.483 kb on - strand, within Pf6N2E2_1468 at 1718.485 kb on - strand, within Pf6N2E2_1468 at 1718.485 kb on - strand, within Pf6N2E2_1468 at 1718.485 kb on - strand, within Pf6N2E2_1468 at 1718.594 kb on - strand, within Pf6N2E2_1468 at 1718.625 kb on + strand, within Pf6N2E2_1468 at 1718.625 kb on + strand, within Pf6N2E2_1468 at 1718.625 kb on + strand, within Pf6N2E2_1468 at 1718.625 kb on + strand, within Pf6N2E2_1468 at 1718.625 kb on + strand, within Pf6N2E2_1468 at 1718.626 kb on - strand, within Pf6N2E2_1468 at 1718.626 kb on - strand, within Pf6N2E2_1468 at 1718.626 kb on - strand, within Pf6N2E2_1468 at 1718.626 kb on - strand, within Pf6N2E2_1468 at 1718.631 kb on + strand, within Pf6N2E2_1468 at 1718.631 kb on + strand, within Pf6N2E2_1468 at 1718.631 kb on + strand, within Pf6N2E2_1468 at 1718.632 kb on - strand, within Pf6N2E2_1468
Per-strain Table
Position Strand Gene LocusTag Fraction Gjerstad humics 0.5 M remove 1,713,915 - Pf6N2E2_1465 0.78 -3.0 1,713,976 + Pf6N2E2_1465 0.83 +0.0 1,713,976 + Pf6N2E2_1465 0.83 -1.4 1,713,976 + Pf6N2E2_1465 0.83 -0.8 1,713,976 + Pf6N2E2_1465 0.83 +1.2 1,714,000 - Pf6N2E2_1465 0.85 -0.7 1,714,000 - Pf6N2E2_1465 0.85 +0.1 1,714,074 + +0.5 1,714,074 + -0.6 1,714,074 + +1.1 1,714,075 - +0.1 1,714,075 - -0.5 1,714,075 - -0.6 1,714,075 - -0.1 1,714,079 + -2.0 1,714,079 + -1.0 1,714,079 + -0.6 1,714,080 - +0.7 1,714,080 - -0.3 1,714,080 - +0.4 1,714,080 - -0.9 1,714,094 + +0.3 1,714,094 + +0.3 1,714,094 + +1.0 1,714,095 - -0.3 1,714,448 - +1.0 1,714,448 - -2.0 1,714,459 + +0.0 1,714,459 + +0.6 1,714,459 + -1.6 1,714,460 - +0.0 1,714,460 - +1.5 1,714,487 - +0.0 1,714,611 - +0.1 1,714,624 + -0.6 1,714,624 + -1.8 1,714,635 - +0.2 1,714,785 + Pf6N2E2_1466 0.77 +0.5 1,714,785 + Pf6N2E2_1466 0.77 +0.6 1,714,785 + Pf6N2E2_1466 0.77 +0.5 1,714,785 + Pf6N2E2_1466 0.77 +0.6 1,714,785 + Pf6N2E2_1466 0.77 +0.2 1,714,785 + Pf6N2E2_1466 0.77 +1.1 1,714,786 - Pf6N2E2_1466 0.77 +0.2 1,714,786 - Pf6N2E2_1466 0.77 -0.6 1,714,786 - Pf6N2E2_1466 0.77 -0.5 1,714,786 - Pf6N2E2_1466 0.77 -0.7 1,714,786 - Pf6N2E2_1466 0.77 +0.8 1,714,786 - Pf6N2E2_1466 0.77 +0.0 1,714,786 - Pf6N2E2_1466 0.77 +0.2 1,714,809 - Pf6N2E2_1466 0.89 +0.2 1,714,809 - Pf6N2E2_1466 0.89 +2.7 1,714,809 - Pf6N2E2_1466 0.89 -0.3 1,714,834 + -1.0 1,714,987 + -0.9 1,714,987 + +1.0 1,714,987 + -0.3 1,714,987 + -0.6 1,714,988 - +1.0 1,714,988 - +0.9 1,715,068 + +0.0 1,715,069 - +0.5 1,715,257 + Pf6N2E2_1467 0.14 -1.0 1,715,258 - Pf6N2E2_1467 0.14 -1.8 1,715,258 - Pf6N2E2_1467 0.14 -0.7 1,715,361 + Pf6N2E2_1467 0.18 +0.4 1,715,361 + Pf6N2E2_1467 0.18 +0.6 1,715,518 + Pf6N2E2_1467 0.23 +0.6 1,715,518 + Pf6N2E2_1467 0.23 -1.8 1,715,519 - Pf6N2E2_1467 0.23 -0.3 1,716,112 + Pf6N2E2_1467 0.44 +1.4 1,716,112 + Pf6N2E2_1467 0.44 -0.8 1,716,113 - Pf6N2E2_1467 0.44 -0.4 1,716,113 - Pf6N2E2_1467 0.44 -1.0 1,716,113 - Pf6N2E2_1467 0.44 -0.3 1,716,115 + Pf6N2E2_1467 0.44 +0.1 1,716,115 + Pf6N2E2_1467 0.44 +0.2 1,716,116 - Pf6N2E2_1467 0.44 -0.2 1,716,116 - Pf6N2E2_1467 0.44 -1.3 1,716,116 - Pf6N2E2_1467 0.44 -1.3 1,716,116 - Pf6N2E2_1467 0.44 +1.0 1,716,116 - Pf6N2E2_1467 0.44 +0.2 1,716,116 - Pf6N2E2_1467 0.44 +0.4 1,716,142 + Pf6N2E2_1467 0.45 +0.4 1,716,142 + Pf6N2E2_1467 0.45 -0.5 1,716,143 - Pf6N2E2_1467 0.45 -1.2 1,716,648 + Pf6N2E2_1467 0.62 +0.8 1,716,667 + Pf6N2E2_1467 0.63 +0.1 1,716,667 + Pf6N2E2_1467 0.63 +0.0 1,716,668 - Pf6N2E2_1467 0.63 +0.3 1,716,668 - Pf6N2E2_1467 0.63 +0.8 1,716,725 - Pf6N2E2_1467 0.65 -0.2 1,716,760 + Pf6N2E2_1467 0.66 +0.9 1,716,979 + Pf6N2E2_1467 0.74 +1.1 1,716,992 + Pf6N2E2_1467 0.74 -0.1 1,717,393 + Pf6N2E2_1467 0.88 -0.2 1,717,394 - Pf6N2E2_1467 0.88 +0.0 1,717,394 - Pf6N2E2_1467 0.88 +0.3 1,717,404 + Pf6N2E2_1467 0.89 +0.8 1,717,405 - Pf6N2E2_1467 0.89 -0.5 1,717,405 - Pf6N2E2_1467 0.89 +0.5 1,717,405 - Pf6N2E2_1467 0.89 +0.2 1,717,830 + +0.3 1,717,831 - +0.7 1,718,085 + Pf6N2E2_1468 0.23 -0.6 1,718,311 - Pf6N2E2_1468 0.38 -0.3 1,718,482 + Pf6N2E2_1468 0.49 +1.0 1,718,482 + Pf6N2E2_1468 0.49 +0.0 1,718,483 - Pf6N2E2_1468 0.49 -0.1 1,718,485 - Pf6N2E2_1468 0.49 -0.2 1,718,485 - Pf6N2E2_1468 0.49 -0.4 1,718,485 - Pf6N2E2_1468 0.49 +0.4 1,718,594 - Pf6N2E2_1468 0.56 -0.2 1,718,625 + Pf6N2E2_1468 0.58 +1.5 1,718,625 + Pf6N2E2_1468 0.58 +0.3 1,718,625 + Pf6N2E2_1468 0.58 +0.1 1,718,625 + Pf6N2E2_1468 0.58 -0.6 1,718,625 + Pf6N2E2_1468 0.58 -0.6 1,718,626 - Pf6N2E2_1468 0.58 -0.3 1,718,626 - Pf6N2E2_1468 0.58 -0.2 1,718,626 - Pf6N2E2_1468 0.58 -1.8 1,718,626 - Pf6N2E2_1468 0.58 -0.2 1,718,631 + Pf6N2E2_1468 0.58 +0.1 1,718,631 + Pf6N2E2_1468 0.58 -0.5 1,718,631 + Pf6N2E2_1468 0.58 -0.6 1,718,632 - Pf6N2E2_1468 0.58 +1.2
Or see this region's nucleotide sequence