Experiment: Gjerstad humics 0.5 M
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1156 and Pf6N2E2_1157 are separated by 39 nucleotides Pf6N2E2_1157 and Pf6N2E2_1158 are separated by 92 nucleotides Pf6N2E2_1158 and Pf6N2E2_1159 are separated by 250 nucleotides
Pf6N2E2_1156 - hypothetical protein, at 1,353,678 to 1,354,211
_1156
Pf6N2E2_1157 - hypothetical protein possibly connected to lactam utilization and allophanate hydrolase, at 1,354,251 to 1,355,042
_1157
Pf6N2E2_1158 - Cyn operon transcriptional activator, at 1,355,135 to 1,356,082
_1158
Pf6N2E2_1159 - Sialic acid transporter (permease) NanT, at 1,356,333 to 1,357,628
_1159
Position (kb)
1355
1356
1357 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1354.180 kb on + strand at 1354.180 kb on + strand at 1354.180 kb on + strand at 1354.181 kb on - strand at 1354.253 kb on - strand at 1354.270 kb on - strand at 1354.350 kb on + strand, within Pf6N2E2_1157 at 1354.350 kb on + strand, within Pf6N2E2_1157 at 1354.408 kb on + strand, within Pf6N2E2_1157 at 1354.461 kb on + strand, within Pf6N2E2_1157 at 1354.762 kb on + strand, within Pf6N2E2_1157 at 1354.779 kb on + strand, within Pf6N2E2_1157 at 1354.780 kb on - strand, within Pf6N2E2_1157 at 1354.973 kb on - strand at 1355.109 kb on + strand at 1355.109 kb on + strand at 1355.130 kb on - strand at 1355.463 kb on + strand, within Pf6N2E2_1158 at 1355.463 kb on + strand, within Pf6N2E2_1158 at 1355.464 kb on - strand, within Pf6N2E2_1158 at 1355.464 kb on - strand, within Pf6N2E2_1158 at 1355.464 kb on - strand, within Pf6N2E2_1158 at 1355.477 kb on + strand, within Pf6N2E2_1158 at 1355.478 kb on - strand, within Pf6N2E2_1158 at 1355.551 kb on - strand, within Pf6N2E2_1158 at 1355.562 kb on + strand, within Pf6N2E2_1158 at 1355.562 kb on + strand, within Pf6N2E2_1158 at 1355.563 kb on - strand, within Pf6N2E2_1158 at 1355.789 kb on - strand, within Pf6N2E2_1158 at 1355.937 kb on - strand, within Pf6N2E2_1158 at 1356.038 kb on + strand at 1356.110 kb on + strand at 1356.110 kb on + strand at 1356.110 kb on + strand at 1356.111 kb on - strand at 1356.111 kb on - strand at 1356.111 kb on - strand at 1356.111 kb on - strand at 1356.171 kb on + strand at 1356.425 kb on + strand at 1356.425 kb on + strand at 1356.425 kb on + strand at 1356.425 kb on + strand at 1356.687 kb on + strand, within Pf6N2E2_1159 at 1356.687 kb on + strand, within Pf6N2E2_1159 at 1356.687 kb on + strand, within Pf6N2E2_1159 at 1356.687 kb on + strand, within Pf6N2E2_1159 at 1356.855 kb on + strand, within Pf6N2E2_1159 at 1356.856 kb on - strand, within Pf6N2E2_1159
Per-strain Table
Position Strand Gene LocusTag Fraction Gjerstad humics 0.5 M remove 1,354,180 + -1.8 1,354,180 + -0.1 1,354,180 + +0.3 1,354,181 - -0.4 1,354,253 - +0.8 1,354,270 - +0.3 1,354,350 + Pf6N2E2_1157 0.12 +0.2 1,354,350 + Pf6N2E2_1157 0.12 -0.4 1,354,408 + Pf6N2E2_1157 0.20 -1.2 1,354,461 + Pf6N2E2_1157 0.27 +0.3 1,354,762 + Pf6N2E2_1157 0.65 -0.1 1,354,779 + Pf6N2E2_1157 0.67 +0.7 1,354,780 - Pf6N2E2_1157 0.67 -0.6 1,354,973 - -1.5 1,355,109 + +0.4 1,355,109 + +0.5 1,355,130 - +1.0 1,355,463 + Pf6N2E2_1158 0.35 -0.9 1,355,463 + Pf6N2E2_1158 0.35 +0.1 1,355,464 - Pf6N2E2_1158 0.35 +0.1 1,355,464 - Pf6N2E2_1158 0.35 -1.3 1,355,464 - Pf6N2E2_1158 0.35 +0.3 1,355,477 + Pf6N2E2_1158 0.36 -0.4 1,355,478 - Pf6N2E2_1158 0.36 -0.4 1,355,551 - Pf6N2E2_1158 0.44 -0.3 1,355,562 + Pf6N2E2_1158 0.45 +0.7 1,355,562 + Pf6N2E2_1158 0.45 +0.7 1,355,563 - Pf6N2E2_1158 0.45 -0.7 1,355,789 - Pf6N2E2_1158 0.69 -0.5 1,355,937 - Pf6N2E2_1158 0.85 -0.3 1,356,038 + -0.2 1,356,110 + +0.3 1,356,110 + +2.2 1,356,110 + +0.5 1,356,111 - +0.5 1,356,111 - -1.8 1,356,111 - +1.0 1,356,111 - -0.0 1,356,171 + -0.5 1,356,425 + +0.4 1,356,425 + -1.5 1,356,425 + -0.3 1,356,425 + +0.1 1,356,687 + Pf6N2E2_1159 0.27 -1.2 1,356,687 + Pf6N2E2_1159 0.27 +0.1 1,356,687 + Pf6N2E2_1159 0.27 -0.6 1,356,687 + Pf6N2E2_1159 0.27 -0.3 1,356,855 + Pf6N2E2_1159 0.40 -0.5 1,356,856 - Pf6N2E2_1159 0.40 -0.0
Or see this region's nucleotide sequence