Experiment: L-Asparagine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c23110 and PGA1_c23120 are separated by 18 nucleotides PGA1_c23120 and PGA1_c23130 are separated by 63 nucleotides PGA1_c23130 and PGA1_c23140 are separated by 73 nucleotides
PGA1_c23110: PGA1_c23110 - putative sugar ABC transporter, extracellular solute-binding protein, at 2,395,064 to 2,396,908
_c23110
PGA1_c23120: PGA1_c23120 - putative sorbitol dehydrogenase, at 2,396,927 to 2,397,736
_c23120
PGA1_c23130: PGA1_c23130 - putative metallopeptidase, family M24, at 2,397,800 to 2,399,110
_c23130
PGA1_c23140: PGA1_c23140 - acetoin:2,6-dichlorophenolindophenol oxidoreductase subunit alpha, at 2,399,184 to 2,400,179
_c23140
Position (kb)
2397
2398
2399
2400 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2396.906 kb on - strand at 2396.918 kb on + strand at 2396.924 kb on + strand at 2396.949 kb on - strand at 2396.982 kb on + strand at 2396.987 kb on + strand at 2396.987 kb on + strand at 2396.987 kb on + strand at 2396.987 kb on + strand at 2396.987 kb on + strand at 2396.987 kb on + strand at 2396.987 kb on + strand at 2396.987 kb on + strand at 2396.987 kb on + strand at 2396.995 kb on - strand at 2396.995 kb on - strand at 2396.995 kb on - strand at 2396.995 kb on - strand at 2396.995 kb on - strand at 2397.014 kb on + strand, within PGA1_c23120 at 2397.021 kb on + strand, within PGA1_c23120 at 2397.035 kb on - strand, within PGA1_c23120 at 2397.059 kb on + strand, within PGA1_c23120 at 2397.079 kb on + strand, within PGA1_c23120 at 2397.130 kb on - strand, within PGA1_c23120 at 2397.140 kb on + strand, within PGA1_c23120 at 2397.167 kb on + strand, within PGA1_c23120 at 2397.167 kb on + strand, within PGA1_c23120 at 2397.175 kb on - strand, within PGA1_c23120 at 2397.175 kb on - strand, within PGA1_c23120 at 2397.197 kb on - strand, within PGA1_c23120 at 2397.197 kb on - strand, within PGA1_c23120 at 2397.197 kb on - strand, within PGA1_c23120 at 2397.199 kb on + strand, within PGA1_c23120 at 2397.199 kb on + strand, within PGA1_c23120 at 2397.207 kb on - strand, within PGA1_c23120 at 2397.207 kb on - strand, within PGA1_c23120 at 2397.207 kb on - strand, within PGA1_c23120 at 2397.207 kb on - strand, within PGA1_c23120 at 2397.212 kb on - strand, within PGA1_c23120 at 2397.217 kb on - strand, within PGA1_c23120 at 2397.255 kb on + strand, within PGA1_c23120 at 2397.308 kb on - strand, within PGA1_c23120 at 2397.372 kb on + strand, within PGA1_c23120 at 2397.412 kb on + strand, within PGA1_c23120 at 2397.435 kb on + strand, within PGA1_c23120 at 2397.435 kb on + strand, within PGA1_c23120 at 2397.435 kb on + strand, within PGA1_c23120 at 2397.435 kb on + strand, within PGA1_c23120 at 2397.443 kb on - strand, within PGA1_c23120 at 2397.443 kb on - strand, within PGA1_c23120 at 2397.443 kb on - strand, within PGA1_c23120 at 2397.467 kb on - strand, within PGA1_c23120 at 2397.508 kb on - strand, within PGA1_c23120 at 2397.512 kb on + strand, within PGA1_c23120 at 2397.515 kb on - strand, within PGA1_c23120 at 2397.569 kb on - strand, within PGA1_c23120 at 2397.578 kb on + strand, within PGA1_c23120 at 2397.578 kb on + strand, within PGA1_c23120 at 2397.578 kb on + strand, within PGA1_c23120 at 2397.578 kb on + strand, within PGA1_c23120 at 2397.586 kb on - strand, within PGA1_c23120 at 2397.586 kb on - strand, within PGA1_c23120 at 2397.641 kb on - strand, within PGA1_c23120 at 2397.671 kb on + strand at 2397.724 kb on + strand at 2397.729 kb on + strand at 2397.732 kb on - strand at 2397.784 kb on - strand at 2397.809 kb on + strand at 2397.817 kb on - strand at 2397.837 kb on + strand at 2397.846 kb on - strand at 2397.846 kb on - strand at 2397.846 kb on - strand at 2397.846 kb on - strand at 2397.864 kb on + strand at 2398.009 kb on - strand, within PGA1_c23130 at 2398.164 kb on + strand, within PGA1_c23130 at 2398.182 kb on + strand, within PGA1_c23130 at 2398.204 kb on + strand, within PGA1_c23130 at 2398.212 kb on - strand, within PGA1_c23130 at 2398.288 kb on - strand, within PGA1_c23130 at 2398.330 kb on - strand, within PGA1_c23130 at 2398.350 kb on + strand, within PGA1_c23130 at 2398.350 kb on + strand, within PGA1_c23130 at 2398.357 kb on - strand, within PGA1_c23130 at 2398.358 kb on - strand, within PGA1_c23130 at 2398.360 kb on + strand, within PGA1_c23130 at 2398.383 kb on - strand, within PGA1_c23130 at 2398.574 kb on - strand, within PGA1_c23130 at 2398.615 kb on + strand, within PGA1_c23130 at 2398.732 kb on + strand, within PGA1_c23130 at 2398.734 kb on - strand, within PGA1_c23130 at 2398.756 kb on + strand, within PGA1_c23130 at 2398.764 kb on + strand, within PGA1_c23130 at 2398.790 kb on - strand, within PGA1_c23130 at 2398.827 kb on + strand, within PGA1_c23130 at 2398.853 kb on + strand, within PGA1_c23130 at 2398.861 kb on - strand, within PGA1_c23130 at 2398.920 kb on + strand, within PGA1_c23130 at 2398.932 kb on + strand, within PGA1_c23130 at 2398.961 kb on + strand, within PGA1_c23130 at 2399.060 kb on + strand at 2399.104 kb on + strand at 2399.123 kb on - strand at 2399.160 kb on - strand at 2399.160 kb on - strand at 2399.238 kb on + strand at 2399.251 kb on + strand at 2399.261 kb on - strand at 2399.263 kb on + strand at 2399.266 kb on - strand at 2399.280 kb on - strand at 2399.306 kb on - strand, within PGA1_c23140 at 2399.306 kb on - strand, within PGA1_c23140 at 2399.306 kb on - strand, within PGA1_c23140 at 2399.353 kb on + strand, within PGA1_c23140 at 2399.375 kb on - strand, within PGA1_c23140 at 2399.402 kb on + strand, within PGA1_c23140 at 2399.445 kb on + strand, within PGA1_c23140 at 2399.445 kb on + strand, within PGA1_c23140 at 2399.453 kb on - strand, within PGA1_c23140 at 2399.465 kb on - strand, within PGA1_c23140 at 2399.488 kb on - strand, within PGA1_c23140 at 2399.510 kb on - strand, within PGA1_c23140 at 2399.515 kb on - strand, within PGA1_c23140 at 2399.585 kb on + strand, within PGA1_c23140 at 2399.637 kb on + strand, within PGA1_c23140 at 2399.637 kb on + strand, within PGA1_c23140 at 2399.663 kb on + strand, within PGA1_c23140 at 2399.694 kb on + strand, within PGA1_c23140 at 2399.757 kb on - strand, within PGA1_c23140 at 2399.785 kb on + strand, within PGA1_c23140 at 2399.914 kb on + strand, within PGA1_c23140 at 2399.918 kb on + strand, within PGA1_c23140 at 2399.922 kb on - strand, within PGA1_c23140 at 2399.926 kb on - strand, within PGA1_c23140 at 2399.945 kb on + strand, within PGA1_c23140 at 2399.956 kb on + strand, within PGA1_c23140 at 2399.977 kb on - strand, within PGA1_c23140 at 2400.021 kb on - strand, within PGA1_c23140 at 2400.054 kb on - strand, within PGA1_c23140 at 2400.069 kb on + strand, within PGA1_c23140 at 2400.090 kb on + strand at 2400.103 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (C) remove 2,396,906 - +1.0 2,396,918 + +0.3 2,396,924 + -0.1 2,396,949 - -1.3 2,396,982 + -0.3 2,396,987 + +0.3 2,396,987 + +1.0 2,396,987 + -0.6 2,396,987 + +0.0 2,396,987 + -0.9 2,396,987 + -2.3 2,396,987 + -0.9 2,396,987 + +0.3 2,396,987 + +0.8 2,396,995 - -0.3 2,396,995 - -1.5 2,396,995 - +0.5 2,396,995 - +1.3 2,396,995 - +0.4 2,397,014 + PGA1_c23120 0.11 -0.1 2,397,021 + PGA1_c23120 0.12 -0.8 2,397,035 - PGA1_c23120 0.13 +0.0 2,397,059 + PGA1_c23120 0.16 +0.8 2,397,079 + PGA1_c23120 0.19 -0.4 2,397,130 - PGA1_c23120 0.25 -1.0 2,397,140 + PGA1_c23120 0.26 +0.2 2,397,167 + PGA1_c23120 0.30 -0.5 2,397,167 + PGA1_c23120 0.30 -0.2 2,397,175 - PGA1_c23120 0.31 +0.8 2,397,175 - PGA1_c23120 0.31 -0.2 2,397,197 - PGA1_c23120 0.33 +0.3 2,397,197 - PGA1_c23120 0.33 +0.1 2,397,197 - PGA1_c23120 0.33 -1.2 2,397,199 + PGA1_c23120 0.34 -0.1 2,397,199 + PGA1_c23120 0.34 +0.6 2,397,207 - PGA1_c23120 0.35 +0.2 2,397,207 - PGA1_c23120 0.35 -0.5 2,397,207 - PGA1_c23120 0.35 +0.4 2,397,207 - PGA1_c23120 0.35 +0.0 2,397,212 - PGA1_c23120 0.35 -1.3 2,397,217 - PGA1_c23120 0.36 +0.8 2,397,255 + PGA1_c23120 0.40 +0.5 2,397,308 - PGA1_c23120 0.47 -0.3 2,397,372 + PGA1_c23120 0.55 +0.2 2,397,412 + PGA1_c23120 0.60 +0.2 2,397,435 + PGA1_c23120 0.63 -1.1 2,397,435 + PGA1_c23120 0.63 +1.1 2,397,435 + PGA1_c23120 0.63 +0.0 2,397,435 + PGA1_c23120 0.63 +0.2 2,397,443 - PGA1_c23120 0.64 -0.8 2,397,443 - PGA1_c23120 0.64 +0.0 2,397,443 - PGA1_c23120 0.64 -1.3 2,397,467 - PGA1_c23120 0.67 -1.9 2,397,508 - PGA1_c23120 0.72 -1.3 2,397,512 + PGA1_c23120 0.72 -0.2 2,397,515 - PGA1_c23120 0.73 -0.1 2,397,569 - PGA1_c23120 0.79 -0.2 2,397,578 + PGA1_c23120 0.80 -0.3 2,397,578 + PGA1_c23120 0.80 -0.9 2,397,578 + PGA1_c23120 0.80 -0.9 2,397,578 + PGA1_c23120 0.80 -0.5 2,397,586 - PGA1_c23120 0.81 -0.6 2,397,586 - PGA1_c23120 0.81 +1.1 2,397,641 - PGA1_c23120 0.88 +0.0 2,397,671 + -1.4 2,397,724 + -1.3 2,397,729 + -0.5 2,397,732 - -0.1 2,397,784 - -0.2 2,397,809 + -0.5 2,397,817 - +0.1 2,397,837 + -0.3 2,397,846 - -0.1 2,397,846 - -1.1 2,397,846 - +0.5 2,397,846 - +0.5 2,397,864 + -0.3 2,398,009 - PGA1_c23130 0.16 +0.2 2,398,164 + PGA1_c23130 0.28 +0.8 2,398,182 + PGA1_c23130 0.29 -0.1 2,398,204 + PGA1_c23130 0.31 +0.2 2,398,212 - PGA1_c23130 0.31 -0.3 2,398,288 - PGA1_c23130 0.37 +0.3 2,398,330 - PGA1_c23130 0.40 -0.0 2,398,350 + PGA1_c23130 0.42 +0.6 2,398,350 + PGA1_c23130 0.42 +0.5 2,398,357 - PGA1_c23130 0.42 +1.1 2,398,358 - PGA1_c23130 0.43 +1.3 2,398,360 + PGA1_c23130 0.43 +0.5 2,398,383 - PGA1_c23130 0.44 -0.0 2,398,574 - PGA1_c23130 0.59 -0.1 2,398,615 + PGA1_c23130 0.62 -0.4 2,398,732 + PGA1_c23130 0.71 +2.3 2,398,734 - PGA1_c23130 0.71 -0.1 2,398,756 + PGA1_c23130 0.73 +0.5 2,398,764 + PGA1_c23130 0.74 +1.0 2,398,790 - PGA1_c23130 0.76 +0.8 2,398,827 + PGA1_c23130 0.78 -0.9 2,398,853 + PGA1_c23130 0.80 -0.2 2,398,861 - PGA1_c23130 0.81 -0.5 2,398,920 + PGA1_c23130 0.85 +0.7 2,398,932 + PGA1_c23130 0.86 +0.7 2,398,961 + PGA1_c23130 0.89 +0.8 2,399,060 + -0.4 2,399,104 + -0.2 2,399,123 - -0.7 2,399,160 - +0.5 2,399,160 - +0.5 2,399,238 + -0.5 2,399,251 + +0.3 2,399,261 - -1.1 2,399,263 + +0.9 2,399,266 - +0.1 2,399,280 - -0.1 2,399,306 - PGA1_c23140 0.12 -0.6 2,399,306 - PGA1_c23140 0.12 +0.5 2,399,306 - PGA1_c23140 0.12 -0.3 2,399,353 + PGA1_c23140 0.17 -0.1 2,399,375 - PGA1_c23140 0.19 +2.4 2,399,402 + PGA1_c23140 0.22 +0.1 2,399,445 + PGA1_c23140 0.26 -0.4 2,399,445 + PGA1_c23140 0.26 -0.8 2,399,453 - PGA1_c23140 0.27 +1.0 2,399,465 - PGA1_c23140 0.28 +1.1 2,399,488 - PGA1_c23140 0.31 +0.7 2,399,510 - PGA1_c23140 0.33 +0.9 2,399,515 - PGA1_c23140 0.33 +1.0 2,399,585 + PGA1_c23140 0.40 +0.0 2,399,637 + PGA1_c23140 0.45 +0.5 2,399,637 + PGA1_c23140 0.45 -0.5 2,399,663 + PGA1_c23140 0.48 -0.1 2,399,694 + PGA1_c23140 0.51 -0.5 2,399,757 - PGA1_c23140 0.58 -0.2 2,399,785 + PGA1_c23140 0.60 +0.0 2,399,914 + PGA1_c23140 0.73 +0.9 2,399,918 + PGA1_c23140 0.74 -0.7 2,399,922 - PGA1_c23140 0.74 -0.5 2,399,926 - PGA1_c23140 0.74 +1.5 2,399,945 + PGA1_c23140 0.76 +0.7 2,399,956 + PGA1_c23140 0.78 -0.5 2,399,977 - PGA1_c23140 0.80 +1.5 2,400,021 - PGA1_c23140 0.84 -1.3 2,400,054 - PGA1_c23140 0.87 +0.5 2,400,069 + PGA1_c23140 0.89 -0.2 2,400,090 + +0.5 2,400,103 - -1.6
Or see this region's nucleotide sequence