Experiment: L-Asparagine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20870 and BWI76_RS20875 are separated by 27 nucleotides BWI76_RS20875 and BWI76_RS20880 are separated by 232 nucleotides BWI76_RS20880 and BWI76_RS20885 are separated by 77 nucleotides
BWI76_RS20870: BWI76_RS20870 - succinyl-diaminopimelate desuccinylase, at 4,364,225 to 4,365,352
_RS20870
BWI76_RS20875: BWI76_RS20875 - membrane protein, at 4,365,380 to 4,365,583
_RS20875
BWI76_RS20880: BWI76_RS20880 - esterase, at 4,365,816 to 4,366,514
_RS20880
BWI76_RS20885: BWI76_RS20885 - tRNA(Met) cytidine acetyltransferase TmcA, at 4,366,592 to 4,368,592
_RS20885
Position (kb) 4365
4366
4367 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4365.337 kb on + strand at 4365.407 kb on + strand, within BWI76_RS20875 at 4366.189 kb on - strand, within BWI76_RS20880 at 4366.394 kb on + strand, within BWI76_RS20880 at 4366.824 kb on - strand at 4366.831 kb on - strand, within BWI76_RS20885 at 4367.071 kb on + strand, within BWI76_RS20885 at 4367.079 kb on - strand, within BWI76_RS20885
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N) remove 4,365,337 + -2.5 4,365,407 + BWI76_RS20875 0.13 +1.6 4,366,189 - BWI76_RS20880 0.53 -2.3 4,366,394 + BWI76_RS20880 0.83 -3.4 4,366,824 - -1.3 4,366,831 - BWI76_RS20885 0.12 +0.1 4,367,071 + BWI76_RS20885 0.24 +2.0 4,367,079 - BWI76_RS20885 0.24 +1.0
Or see this region's nucleotide sequence