Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS30960 and AZOBR_RS30965 are separated by 64 nucleotides AZOBR_RS30965 and AZOBR_RS30970 overlap by 4 nucleotides AZOBR_RS30970 and AZOBR_RS30975 are separated by 201 nucleotides
AZOBR_RS30960: AZOBR_RS30960 - thiazole synthase, at 315,556 to 316,308
_RS30960
AZOBR_RS30965: AZOBR_RS30965 - thiamine biosynthesis protein ThiS, at 316,373 to 316,582
_RS30965
AZOBR_RS30970: AZOBR_RS30970 - glycine oxidase, at 316,579 to 317,703
_RS30970
AZOBR_RS30975: AZOBR_RS30975 - magnesium transporter, at 317,905 to 319,344
_RS30975
Position (kb) 316
317
318 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 316.727 kb on + strand, within AZOBR_RS30970 at 316.727 kb on + strand, within AZOBR_RS30970 at 316.955 kb on + strand, within AZOBR_RS30970 at 316.955 kb on + strand, within AZOBR_RS30970 at 316.955 kb on + strand, within AZOBR_RS30970 at 317.192 kb on + strand, within AZOBR_RS30970 at 317.192 kb on + strand, within AZOBR_RS30970 at 317.193 kb on - strand, within AZOBR_RS30970 at 317.193 kb on - strand, within AZOBR_RS30970 at 317.759 kb on - strand at 317.759 kb on - strand at 318.241 kb on + strand, within AZOBR_RS30975 at 318.242 kb on - strand, within AZOBR_RS30975 at 318.428 kb on + strand, within AZOBR_RS30975 at 318.428 kb on + strand, within AZOBR_RS30975 at 318.428 kb on + strand, within AZOBR_RS30975 at 318.428 kb on + strand, within AZOBR_RS30975 at 318.569 kb on + strand, within AZOBR_RS30975
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB remove 316,727 + AZOBR_RS30970 0.13 -3.5 316,727 + AZOBR_RS30970 0.13 -1.2 316,955 + AZOBR_RS30970 0.33 +0.5 316,955 + AZOBR_RS30970 0.33 -3.4 316,955 + AZOBR_RS30970 0.33 +0.4 317,192 + AZOBR_RS30970 0.54 -0.7 317,192 + AZOBR_RS30970 0.54 -0.6 317,193 - AZOBR_RS30970 0.55 -0.7 317,193 - AZOBR_RS30970 0.55 -0.5 317,759 - -0.1 317,759 - -0.0 318,241 + AZOBR_RS30975 0.23 -1.3 318,242 - AZOBR_RS30975 0.23 -0.5 318,428 + AZOBR_RS30975 0.36 +0.5 318,428 + AZOBR_RS30975 0.36 -0.2 318,428 + AZOBR_RS30975 0.36 +1.8 318,428 + AZOBR_RS30975 0.36 +0.3 318,569 + AZOBR_RS30975 0.46 +0.8
Or see this region's nucleotide sequence