Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS15055 and AZOBR_RS15060 overlap by 4 nucleotides AZOBR_RS15060 and AZOBR_RS15065 are separated by 82 nucleotides
AZOBR_RS15055: AZOBR_RS15055 - muraminidase, at 150,914 to 151,360
_RS15055
AZOBR_RS15060: AZOBR_RS15060 - hypothetical protein, at 151,357 to 151,536
_RS15060
AZOBR_RS15065: AZOBR_RS15065 - hypothetical protein, at 151,619 to 154,144
_RS15065
Position (kb)
151
152
153
154
155 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 151.302 kb on + strand, within AZOBR_RS15055 at 151.302 kb on + strand, within AZOBR_RS15055 at 151.541 kb on + strand at 151.541 kb on + strand at 151.541 kb on + strand at 151.541 kb on + strand at 151.541 kb on + strand at 151.541 kb on + strand at 151.542 kb on - strand at 151.542 kb on - strand at 151.542 kb on - strand at 151.542 kb on - strand at 151.542 kb on - strand at 151.716 kb on + strand at 151.717 kb on - strand at 151.746 kb on + strand at 151.747 kb on - strand at 152.027 kb on + strand, within AZOBR_RS15065 at 152.028 kb on - strand, within AZOBR_RS15065 at 152.083 kb on - strand, within AZOBR_RS15065 at 152.130 kb on - strand, within AZOBR_RS15065 at 152.437 kb on - strand, within AZOBR_RS15065 at 152.462 kb on - strand, within AZOBR_RS15065 at 152.511 kb on + strand, within AZOBR_RS15065 at 152.511 kb on + strand, within AZOBR_RS15065 at 152.512 kb on - strand, within AZOBR_RS15065 at 152.573 kb on - strand, within AZOBR_RS15065 at 152.574 kb on + strand, within AZOBR_RS15065 at 152.574 kb on + strand, within AZOBR_RS15065 at 152.574 kb on + strand, within AZOBR_RS15065 at 152.574 kb on + strand, within AZOBR_RS15065 at 152.700 kb on + strand, within AZOBR_RS15065 at 152.700 kb on + strand, within AZOBR_RS15065 at 152.700 kb on + strand, within AZOBR_RS15065 at 152.701 kb on - strand, within AZOBR_RS15065 at 152.701 kb on - strand, within AZOBR_RS15065 at 152.749 kb on - strand, within AZOBR_RS15065 at 152.749 kb on - strand, within AZOBR_RS15065 at 152.764 kb on + strand, within AZOBR_RS15065 at 152.772 kb on + strand, within AZOBR_RS15065 at 152.805 kb on + strand, within AZOBR_RS15065 at 152.806 kb on - strand, within AZOBR_RS15065 at 152.856 kb on + strand, within AZOBR_RS15065 at 152.856 kb on + strand, within AZOBR_RS15065 at 152.856 kb on + strand, within AZOBR_RS15065 at 152.857 kb on - strand, within AZOBR_RS15065 at 152.857 kb on - strand, within AZOBR_RS15065 at 152.857 kb on - strand, within AZOBR_RS15065 at 152.975 kb on - strand, within AZOBR_RS15065 at 152.975 kb on - strand, within AZOBR_RS15065 at 153.360 kb on + strand, within AZOBR_RS15065 at 153.361 kb on - strand, within AZOBR_RS15065 at 153.361 kb on - strand, within AZOBR_RS15065 at 153.363 kb on + strand, within AZOBR_RS15065 at 153.363 kb on + strand, within AZOBR_RS15065 at 153.363 kb on + strand, within AZOBR_RS15065 at 153.364 kb on - strand, within AZOBR_RS15065 at 153.364 kb on - strand, within AZOBR_RS15065 at 153.570 kb on + strand, within AZOBR_RS15065 at 153.571 kb on - strand, within AZOBR_RS15065 at 153.571 kb on - strand, within AZOBR_RS15065 at 153.615 kb on - strand, within AZOBR_RS15065 at 153.615 kb on - strand, within AZOBR_RS15065 at 154.101 kb on + strand at 154.101 kb on + strand at 154.102 kb on - strand at 154.210 kb on + strand at 154.215 kb on - strand at 154.296 kb on + strand at 154.346 kb on - strand at 154.346 kb on - strand at 154.393 kb on + strand at 155.032 kb on - strand at 155.042 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB remove 151,302 + AZOBR_RS15055 0.87 +1.4 151,302 + AZOBR_RS15055 0.87 -0.2 151,541 + +1.0 151,541 + +1.1 151,541 + +0.9 151,541 + -0.9 151,541 + +0.8 151,541 + +0.1 151,542 - +1.1 151,542 - -1.1 151,542 - +0.5 151,542 - +0.5 151,542 - +1.8 151,716 + +0.9 151,717 - +1.3 151,746 + +0.6 151,747 - +1.2 152,027 + AZOBR_RS15065 0.16 +1.8 152,028 - AZOBR_RS15065 0.16 +3.6 152,083 - AZOBR_RS15065 0.18 +2.0 152,130 - AZOBR_RS15065 0.20 -0.0 152,437 - AZOBR_RS15065 0.32 -2.2 152,462 - AZOBR_RS15065 0.33 +2.3 152,511 + AZOBR_RS15065 0.35 +0.1 152,511 + AZOBR_RS15065 0.35 +0.7 152,512 - AZOBR_RS15065 0.35 +0.8 152,573 - AZOBR_RS15065 0.38 +1.2 152,574 + AZOBR_RS15065 0.38 +0.5 152,574 + AZOBR_RS15065 0.38 +0.2 152,574 + AZOBR_RS15065 0.38 +1.5 152,574 + AZOBR_RS15065 0.38 +1.6 152,700 + AZOBR_RS15065 0.43 +0.1 152,700 + AZOBR_RS15065 0.43 +0.7 152,700 + AZOBR_RS15065 0.43 +0.8 152,701 - AZOBR_RS15065 0.43 +0.9 152,701 - AZOBR_RS15065 0.43 +1.5 152,749 - AZOBR_RS15065 0.45 +0.4 152,749 - AZOBR_RS15065 0.45 +0.5 152,764 + AZOBR_RS15065 0.45 -0.3 152,772 + AZOBR_RS15065 0.46 +0.9 152,805 + AZOBR_RS15065 0.47 -0.1 152,806 - AZOBR_RS15065 0.47 -0.2 152,856 + AZOBR_RS15065 0.49 +0.7 152,856 + AZOBR_RS15065 0.49 -0.1 152,856 + AZOBR_RS15065 0.49 +1.3 152,857 - AZOBR_RS15065 0.49 -0.3 152,857 - AZOBR_RS15065 0.49 -1.3 152,857 - AZOBR_RS15065 0.49 +0.8 152,975 - AZOBR_RS15065 0.54 +0.2 152,975 - AZOBR_RS15065 0.54 -1.1 153,360 + AZOBR_RS15065 0.69 +0.3 153,361 - AZOBR_RS15065 0.69 +1.1 153,361 - AZOBR_RS15065 0.69 +1.8 153,363 + AZOBR_RS15065 0.69 -2.8 153,363 + AZOBR_RS15065 0.69 +1.7 153,363 + AZOBR_RS15065 0.69 +1.4 153,364 - AZOBR_RS15065 0.69 +0.4 153,364 - AZOBR_RS15065 0.69 +0.6 153,570 + AZOBR_RS15065 0.77 +1.4 153,571 - AZOBR_RS15065 0.77 -0.7 153,571 - AZOBR_RS15065 0.77 +1.0 153,615 - AZOBR_RS15065 0.79 -0.2 153,615 - AZOBR_RS15065 0.79 +0.9 154,101 + +0.3 154,101 + -0.2 154,102 - -1.2 154,210 + -1.1 154,215 - +1.3 154,296 + -0.2 154,346 - +1.7 154,346 - +2.5 154,393 + +2.1 155,032 - +0.4 155,042 - -1.9
Or see this region's nucleotide sequence