Strain Fitness in Azospirillum brasilense Sp245 around AZOBR_RS00660

Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAZOBR_RS00650 and AZOBR_RS00655 overlap by 8 nucleotidesAZOBR_RS00655 and AZOBR_RS00660 overlap by 1 nucleotidesAZOBR_RS00660 and AZOBR_RS00665 are separated by 206 nucleotidesAZOBR_RS00665 and AZOBR_RS00670 are separated by 140 nucleotides AZOBR_RS00650: AZOBR_RS00650 - 5 10-methylene-tetrahydrofolate dehydrogenase, at 130,573 to 131,481 _RS00650 AZOBR_RS00655: AZOBR_RS00655 - hypothetical protein, at 131,474 to 131,833 _RS00655 AZOBR_RS00660: AZOBR_RS00660 - hypothetical protein, at 131,833 to 132,117 _RS00660 AZOBR_RS00665: AZOBR_RS00665 - tRNA-Ala, at 132,324 to 132,399 _RS00665 AZOBR_RS00670: AZOBR_RS00670 - membrane protein, at 132,540 to 133,586 _RS00670 Position (kb) 131 132 133Strain fitness (log2 ratio) -2 -1 0 1at 131.849 kb on + strandat 132.075 kb on - strand, within AZOBR_RS00660at 132.366 kb on + strand, within AZOBR_RS00665at 132.367 kb on - strand, within AZOBR_RS00665at 132.367 kb on - strand, within AZOBR_RS00665at 132.847 kb on + strand, within AZOBR_RS00670at 132.848 kb on - strand, within AZOBR_RS00670at 132.848 kb on - strand, within AZOBR_RS00670at 132.913 kb on + strand, within AZOBR_RS00670at 132.914 kb on - strand, within AZOBR_RS00670

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Per-strain Table

Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB
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131,849 + -1.7
132,075 - AZOBR_RS00660 0.85 -0.1
132,366 + AZOBR_RS00665 0.55 +0.5
132,367 - AZOBR_RS00665 0.57 -2.0
132,367 - AZOBR_RS00665 0.57 -0.4
132,847 + AZOBR_RS00670 0.29 -0.8
132,848 - AZOBR_RS00670 0.29 +0.1
132,848 - AZOBR_RS00670 0.29 +1.3
132,913 + AZOBR_RS00670 0.36 -0.1
132,914 - AZOBR_RS00670 0.36 +0.3

Or see this region's nucleotide sequence