Experiment: BHIS with Dimetridazole 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4026 and BT4027 are separated by 26 nucleotides BT4027 and BT4028 overlap by 4 nucleotides BT4028 and BT4029 are separated by 143 nucleotides
BT4026: BT4026 - conserved hypothetical protein (NCBI ptt file), at 5,241,062 to 5,242,060
BT4026
BT4027: BT4027 - hypothetical protein (NCBI ptt file), at 5,242,087 to 5,242,314
BT4027
BT4028: BT4028 - hypothetical protein (NCBI ptt file), at 5,242,311 to 5,242,583
BT4028
BT4029: BT4029 - hypothetical protein (NCBI ptt file), at 5,242,727 to 5,243,467
BT4029
Position (kb) 5242
5243 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5241.123 kb on + strand at 5241.124 kb on - strand at 5241.158 kb on + strand at 5241.167 kb on + strand, within BT4026 at 5241.209 kb on + strand, within BT4026 at 5241.210 kb on - strand, within BT4026 at 5241.345 kb on + strand, within BT4026 at 5241.345 kb on + strand, within BT4026 at 5241.415 kb on - strand, within BT4026 at 5241.436 kb on - strand, within BT4026 at 5241.512 kb on - strand, within BT4026 at 5241.655 kb on + strand, within BT4026 at 5241.715 kb on + strand, within BT4026 at 5241.716 kb on - strand, within BT4026 at 5241.721 kb on - strand, within BT4026 at 5241.737 kb on - strand, within BT4026 at 5241.886 kb on - strand, within BT4026 at 5241.931 kb on + strand, within BT4026 at 5241.931 kb on + strand, within BT4026 at 5241.978 kb on + strand at 5242.098 kb on + strand at 5242.117 kb on + strand, within BT4027 at 5242.118 kb on - strand, within BT4027 at 5242.138 kb on - strand, within BT4027 at 5242.139 kb on + strand, within BT4027 at 5242.140 kb on - strand, within BT4027 at 5242.423 kb on - strand, within BT4028 at 5242.582 kb on - strand at 5242.615 kb on + strand at 5242.739 kb on - strand at 5242.760 kb on - strand at 5242.821 kb on - strand, within BT4029 at 5243.118 kb on + strand, within BT4029 at 5243.121 kb on + strand, within BT4029 at 5243.157 kb on + strand, within BT4029 at 5243.164 kb on - strand, within BT4029 at 5243.272 kb on - strand, within BT4029
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimetridazole 0.025 mM remove 5,241,123 + -2.8 5,241,124 - -0.9 5,241,158 + -2.5 5,241,167 + BT4026 0.11 -1.8 5,241,209 + BT4026 0.15 +1.0 5,241,210 - BT4026 0.15 -0.4 5,241,345 + BT4026 0.28 -1.3 5,241,345 + BT4026 0.28 -2.4 5,241,415 - BT4026 0.35 -1.6 5,241,436 - BT4026 0.37 +3.3 5,241,512 - BT4026 0.45 -1.0 5,241,655 + BT4026 0.59 -0.5 5,241,715 + BT4026 0.65 -1.0 5,241,716 - BT4026 0.65 -0.9 5,241,721 - BT4026 0.66 -0.9 5,241,737 - BT4026 0.68 -1.3 5,241,886 - BT4026 0.82 +0.0 5,241,931 + BT4026 0.87 +2.0 5,241,931 + BT4026 0.87 -2.1 5,241,978 + -1.0 5,242,098 + +1.0 5,242,117 + BT4027 0.13 -2.3 5,242,118 - BT4027 0.14 -2.2 5,242,138 - BT4027 0.22 -2.0 5,242,139 + BT4027 0.23 +1.0 5,242,140 - BT4027 0.23 +0.0 5,242,423 - BT4028 0.41 -1.5 5,242,582 - -1.4 5,242,615 + -0.2 5,242,739 - +1.0 5,242,760 - -1.0 5,242,821 - BT4029 0.13 -2.6 5,243,118 + BT4029 0.53 +1.0 5,243,121 + BT4029 0.53 -2.0 5,243,157 + BT4029 0.58 -0.6 5,243,164 - BT4029 0.59 -2.5 5,243,272 - BT4029 0.74 -0.3
Or see this region's nucleotide sequence