Experiment: BHIS with Dimetridazole 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT4021 and BT4022 are separated by 21 nucleotides BT4022 and BT4023 are separated by 150 nucleotides BT4023 and BT4024 overlap by 4 nucleotides BT4024 and BT4025 are separated by 21 nucleotides
BT4021: BT4021 - integrase (NCBI ptt file), at 5,237,521 to 5,238,732
BT4021
BT4022: BT4022 - integrase (NCBI ptt file), at 5,238,754 to 5,239,983
BT4022
BT4023: BT4023 - transposase (NCBI ptt file), at 5,240,134 to 5,240,490
BT4023
BT4024: BT4024 - hypothetical protein (NCBI ptt file), at 5,240,487 to 5,240,747
BT4024
BT4025: BT4025 - hypothetical protein (NCBI ptt file), at 5,240,769 to 5,241,065
BT4025
Position (kb)
5238
5239
5240 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5237.777 kb on - strand, within BT4021 at 5237.830 kb on - strand, within BT4021 at 5237.863 kb on + strand, within BT4021 at 5237.863 kb on + strand, within BT4021 at 5237.863 kb on + strand, within BT4021 at 5237.863 kb on + strand, within BT4021 at 5237.864 kb on - strand, within BT4021 at 5237.864 kb on - strand, within BT4021 at 5237.864 kb on - strand, within BT4021 at 5237.972 kb on + strand, within BT4021 at 5237.973 kb on - strand, within BT4021 at 5237.998 kb on + strand, within BT4021 at 5237.999 kb on - strand, within BT4021 at 5238.024 kb on + strand, within BT4021 at 5238.024 kb on + strand, within BT4021 at 5238.025 kb on - strand, within BT4021 at 5238.035 kb on + strand, within BT4021 at 5238.036 kb on - strand, within BT4021 at 5238.036 kb on - strand, within BT4021 at 5238.080 kb on + strand, within BT4021 at 5238.080 kb on + strand, within BT4021 at 5238.081 kb on - strand, within BT4021 at 5238.154 kb on + strand, within BT4021 at 5238.165 kb on - strand, within BT4021 at 5238.510 kb on - strand, within BT4021 at 5238.530 kb on - strand, within BT4021 at 5238.534 kb on - strand, within BT4021 at 5238.608 kb on + strand, within BT4021 at 5238.661 kb on + strand at 5238.710 kb on - strand at 5238.733 kb on - strand at 5238.788 kb on - strand at 5238.876 kb on + strand at 5238.877 kb on - strand, within BT4022 at 5238.877 kb on - strand, within BT4022 at 5238.912 kb on + strand, within BT4022 at 5239.050 kb on + strand, within BT4022 at 5239.051 kb on - strand, within BT4022 at 5239.128 kb on - strand, within BT4022 at 5239.159 kb on - strand, within BT4022 at 5239.162 kb on + strand, within BT4022 at 5239.163 kb on - strand, within BT4022 at 5239.163 kb on - strand, within BT4022 at 5239.196 kb on + strand, within BT4022 at 5239.211 kb on + strand, within BT4022 at 5239.243 kb on + strand, within BT4022 at 5239.344 kb on + strand, within BT4022 at 5239.373 kb on + strand, within BT4022 at 5239.386 kb on - strand, within BT4022 at 5239.386 kb on - strand, within BT4022 at 5239.386 kb on - strand, within BT4022 at 5239.529 kb on + strand, within BT4022 at 5239.529 kb on + strand, within BT4022 at 5239.568 kb on + strand, within BT4022 at 5239.569 kb on - strand at 5239.569 kb on - strand, within BT4022 at 5239.705 kb on - strand, within BT4022 at 5239.961 kb on - strand at 5240.051 kb on - strand at 5240.194 kb on - strand, within BT4023 at 5240.202 kb on - strand, within BT4023 at 5240.223 kb on - strand, within BT4023 at 5240.236 kb on - strand, within BT4023 at 5240.266 kb on - strand, within BT4023 at 5240.266 kb on - strand, within BT4023 at 5240.268 kb on - strand, within BT4023 at 5240.356 kb on - strand, within BT4023 at 5240.554 kb on - strand, within BT4024 at 5240.619 kb on - strand, within BT4024 at 5240.666 kb on - strand, within BT4024 at 5240.666 kb on - strand, within BT4024 at 5240.717 kb on - strand, within BT4024 at 5240.728 kb on - strand at 5240.743 kb on - strand at 5240.753 kb on - strand at 5240.763 kb on - strand at 5240.922 kb on - strand, within BT4025
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimetridazole 0.025 mM remove 5,237,777 - BT4021 0.21 +0.0 5,237,830 - BT4021 0.25 -1.1 5,237,863 + BT4021 0.28 -0.7 5,237,863 + BT4021 0.28 -1.3 5,237,863 + BT4021 0.28 -0.1 5,237,863 + BT4021 0.28 +1.0 5,237,864 - BT4021 0.28 -2.3 5,237,864 - BT4021 0.28 -0.3 5,237,864 - BT4021 0.28 -0.7 5,237,972 + BT4021 0.37 +1.0 5,237,973 - BT4021 0.37 -0.8 5,237,998 + BT4021 0.39 +1.0 5,237,999 - BT4021 0.39 -0.3 5,238,024 + BT4021 0.42 +0.0 5,238,024 + BT4021 0.42 -2.2 5,238,025 - BT4021 0.42 -1.0 5,238,035 + BT4021 0.42 +1.0 5,238,036 - BT4021 0.42 -0.9 5,238,036 - BT4021 0.42 -1.0 5,238,080 + BT4021 0.46 +0.6 5,238,080 + BT4021 0.46 -1.1 5,238,081 - BT4021 0.46 -0.3 5,238,154 + BT4021 0.52 -1.0 5,238,165 - BT4021 0.53 -1.9 5,238,510 - BT4021 0.82 -0.8 5,238,530 - BT4021 0.83 -1.0 5,238,534 - BT4021 0.84 -1.3 5,238,608 + BT4021 0.90 +1.0 5,238,661 + -0.5 5,238,710 - +0.0 5,238,733 - +0.0 5,238,788 - +1.0 5,238,876 + -2.4 5,238,877 - BT4022 0.10 -0.2 5,238,877 - BT4022 0.10 +2.0 5,238,912 + BT4022 0.13 -1.6 5,239,050 + BT4022 0.24 +1.0 5,239,051 - BT4022 0.24 +0.2 5,239,128 - BT4022 0.30 -0.2 5,239,159 - BT4022 0.33 -0.6 5,239,162 + BT4022 0.33 -0.6 5,239,163 - BT4022 0.33 -1.3 5,239,163 - BT4022 0.33 -0.6 5,239,196 + BT4022 0.36 +0.2 5,239,211 + BT4022 0.37 -0.6 5,239,243 + BT4022 0.40 -0.1 5,239,344 + BT4022 0.48 -0.4 5,239,373 + BT4022 0.50 +1.4 5,239,386 - BT4022 0.51 +0.3 5,239,386 - BT4022 0.51 +1.0 5,239,386 - BT4022 0.51 -0.6 5,239,529 + BT4022 0.63 +0.0 5,239,529 + BT4022 0.63 -0.6 5,239,568 + BT4022 0.66 -0.5 5,239,569 - +0.0 5,239,569 - BT4022 0.66 -0.2 5,239,705 - BT4022 0.77 -1.0 5,239,961 - -0.7 5,240,051 - -1.8 5,240,194 - BT4023 0.17 +1.3 5,240,202 - BT4023 0.19 +0.2 5,240,223 - BT4023 0.25 -1.0 5,240,236 - BT4023 0.29 -0.6 5,240,266 - BT4023 0.37 -0.6 5,240,266 - BT4023 0.37 +0.3 5,240,268 - BT4023 0.38 -0.4 5,240,356 - BT4023 0.62 -1.9 5,240,554 - BT4024 0.26 +1.0 5,240,619 - BT4024 0.51 -2.0 5,240,666 - BT4024 0.69 -1.6 5,240,666 - BT4024 0.69 -1.0 5,240,717 - BT4024 0.88 -1.8 5,240,728 - -1.3 5,240,743 - -1.6 5,240,753 - -1.6 5,240,763 - -1.3 5,240,922 - BT4025 0.52 +1.0
Or see this region's nucleotide sequence