Experiment: BHIS with Dimetridazole 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT3372 and BT3373 are separated by 6 nucleotides BT3373 and BT3374 are separated by 3 nucleotides
BT3372: BT3372 - glycosyltransferase (NCBI ptt file), at 4,348,877 to 4,349,857
BT3372
BT3373: BT3373 - spermidine n1-acetyltransferase (NCBI ptt file), at 4,349,864 to 4,350,307
BT3373
BT3374: BT3374 - putative carbamoyl-phosphate-synthetase (NCBI ptt file), at 4,350,311 to 4,351,495
BT3374
Position (kb) 4349
4350
4351 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 4348.900 kb on + strand at 4348.980 kb on + strand, within BT3372 at 4348.985 kb on - strand, within BT3372 at 4349.005 kb on - strand, within BT3372 at 4349.038 kb on + strand, within BT3372 at 4349.039 kb on - strand, within BT3372 at 4349.040 kb on + strand, within BT3372 at 4349.041 kb on - strand, within BT3372 at 4349.171 kb on + strand, within BT3372 at 4349.171 kb on + strand, within BT3372 at 4349.171 kb on + strand, within BT3372 at 4349.214 kb on - strand, within BT3372 at 4349.253 kb on + strand, within BT3372 at 4349.359 kb on + strand, within BT3372 at 4349.387 kb on + strand, within BT3372 at 4349.396 kb on - strand, within BT3372 at 4349.454 kb on + strand, within BT3372 at 4349.455 kb on - strand, within BT3372 at 4349.472 kb on + strand, within BT3372 at 4349.474 kb on + strand, within BT3372 at 4349.475 kb on - strand, within BT3372 at 4349.529 kb on - strand, within BT3372 at 4349.561 kb on + strand, within BT3372 at 4349.612 kb on - strand, within BT3372 at 4349.649 kb on - strand, within BT3372 at 4349.658 kb on - strand, within BT3372 at 4349.792 kb on - strand at 4349.792 kb on - strand at 4349.794 kb on - strand at 4349.794 kb on - strand at 4349.808 kb on + strand at 4349.809 kb on - strand at 4349.828 kb on + strand at 4349.892 kb on - strand at 4349.895 kb on + strand at 4349.936 kb on - strand, within BT3373 at 4349.972 kb on - strand, within BT3373 at 4350.015 kb on - strand, within BT3373 at 4350.015 kb on - strand, within BT3373 at 4350.022 kb on - strand, within BT3373 at 4350.022 kb on - strand, within BT3373 at 4350.078 kb on + strand, within BT3373 at 4350.078 kb on + strand, within BT3373 at 4350.086 kb on - strand, within BT3373 at 4350.089 kb on + strand, within BT3373 at 4350.089 kb on + strand, within BT3373 at 4350.152 kb on - strand, within BT3373 at 4350.152 kb on - strand, within BT3373 at 4350.157 kb on - strand, within BT3373 at 4350.160 kb on - strand, within BT3373 at 4350.274 kb on - strand at 4350.274 kb on - strand at 4350.322 kb on + strand at 4350.323 kb on - strand at 4350.330 kb on - strand at 4350.391 kb on + strand at 4350.468 kb on - strand, within BT3374 at 4350.504 kb on - strand, within BT3374 at 4350.532 kb on + strand, within BT3374 at 4350.536 kb on + strand, within BT3374 at 4350.536 kb on + strand, within BT3374 at 4350.537 kb on - strand, within BT3374 at 4350.537 kb on - strand, within BT3374 at 4350.537 kb on - strand, within BT3374 at 4350.537 kb on - strand, within BT3374 at 4350.689 kb on + strand, within BT3374 at 4350.703 kb on - strand, within BT3374 at 4350.782 kb on + strand, within BT3374 at 4350.876 kb on + strand, within BT3374 at 4350.876 kb on + strand, within BT3374 at 4350.877 kb on - strand, within BT3374 at 4351.013 kb on + strand, within BT3374 at 4351.014 kb on - strand, within BT3374 at 4351.049 kb on - strand, within BT3374 at 4351.104 kb on - strand, within BT3374 at 4351.134 kb on + strand, within BT3374 at 4351.229 kb on - strand, within BT3374
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimetridazole 0.025 mM remove 4,348,900 + -0.6 4,348,980 + BT3372 0.10 -1.1 4,348,985 - BT3372 0.11 -0.1 4,349,005 - BT3372 0.13 -1.4 4,349,038 + BT3372 0.16 -2.4 4,349,039 - BT3372 0.17 -3.2 4,349,040 + BT3372 0.17 +0.9 4,349,041 - BT3372 0.17 -2.4 4,349,171 + BT3372 0.30 -1.6 4,349,171 + BT3372 0.30 -3.2 4,349,171 + BT3372 0.30 -1.6 4,349,214 - BT3372 0.34 -1.6 4,349,253 + BT3372 0.38 +0.9 4,349,359 + BT3372 0.49 -2.4 4,349,387 + BT3372 0.52 -0.6 4,349,396 - BT3372 0.53 -5.0 4,349,454 + BT3372 0.59 -0.6 4,349,455 - BT3372 0.59 -0.1 4,349,472 + BT3372 0.61 +0.9 4,349,474 + BT3372 0.61 -1.3 4,349,475 - BT3372 0.61 -3.1 4,349,529 - BT3372 0.66 -3.1 4,349,561 + BT3372 0.70 -1.4 4,349,612 - BT3372 0.75 -2.4 4,349,649 - BT3372 0.79 +0.4 4,349,658 - BT3372 0.80 -1.4 4,349,792 - -1.5 4,349,792 - -2.5 4,349,794 - +0.9 4,349,794 - -2.0 4,349,808 + -1.1 4,349,809 - -2.2 4,349,828 + +0.9 4,349,892 - -2.1 4,349,895 + -0.1 4,349,936 - BT3373 0.16 -0.1 4,349,972 - BT3373 0.24 -1.4 4,350,015 - BT3373 0.34 -2.4 4,350,015 - BT3373 0.34 +0.9 4,350,022 - BT3373 0.36 -2.1 4,350,022 - BT3373 0.36 -2.2 4,350,078 + BT3373 0.48 -3.6 4,350,078 + BT3373 0.48 -2.6 4,350,086 - BT3373 0.50 +0.9 4,350,089 + BT3373 0.51 -2.5 4,350,089 + BT3373 0.51 +0.9 4,350,152 - BT3373 0.65 -2.6 4,350,152 - BT3373 0.65 -2.2 4,350,157 - BT3373 0.66 -1.1 4,350,160 - BT3373 0.67 -1.9 4,350,274 - -0.1 4,350,274 - -3.3 4,350,322 + -0.1 4,350,323 - -0.1 4,350,330 - -2.6 4,350,391 + -1.4 4,350,468 - BT3374 0.13 -2.7 4,350,504 - BT3374 0.16 -1.1 4,350,532 + BT3374 0.19 -1.2 4,350,536 + BT3374 0.19 -2.0 4,350,536 + BT3374 0.19 -0.6 4,350,537 - BT3374 0.19 -1.6 4,350,537 - BT3374 0.19 -1.9 4,350,537 - BT3374 0.19 -1.9 4,350,537 - BT3374 0.19 -0.1 4,350,689 + BT3374 0.32 -1.4 4,350,703 - BT3374 0.33 +0.9 4,350,782 + BT3374 0.40 -2.2 4,350,876 + BT3374 0.48 -0.7 4,350,876 + BT3374 0.48 +0.9 4,350,877 - BT3374 0.48 +0.9 4,351,013 + BT3374 0.59 -1.6 4,351,014 - BT3374 0.59 +0.9 4,351,049 - BT3374 0.62 -0.6 4,351,104 - BT3374 0.67 -0.1 4,351,134 + BT3374 0.69 -1.8 4,351,229 - BT3374 0.77 -0.6
Or see this region's nucleotide sequence