Experiment: BHIS with Dimetridazole 0.025 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT2183 and BT2184 are separated by 194 nucleotides BT2184 and BT2185 overlap by 14 nucleotides BT2185 and BT2186 are separated by 40 nucleotides
BT2183: BT2183 - conserved hypothetical protein (NCBI ptt file), at 2,741,631 to 2,742,686
BT2183
BT2184: BT2184 - RNA polymerase ECF-type sigma factor (NCBI ptt file), at 2,742,881 to 2,743,375
BT2184
BT2185: BT2185 - hypothetical protein (NCBI ptt file), at 2,743,362 to 2,743,952
BT2185
BT2186: BT2186 - 3-phosphoshikimate 1-carboxyvinyltransferase (EC 2.5.1.19) (from data) , at 2,743,993 to 2,745,225
BT2186
Position (kb)
2742
2743
2744 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2741.885 kb on - strand, within BT2183 at 2741.901 kb on + strand, within BT2183 at 2741.902 kb on - strand, within BT2183 at 2741.903 kb on + strand, within BT2183 at 2741.904 kb on - strand, within BT2183 at 2741.945 kb on + strand, within BT2183 at 2741.945 kb on + strand, within BT2183 at 2741.946 kb on - strand, within BT2183 at 2741.998 kb on + strand, within BT2183 at 2742.160 kb on + strand, within BT2183 at 2742.237 kb on - strand, within BT2183 at 2742.237 kb on - strand, within BT2183 at 2742.377 kb on - strand, within BT2183 at 2742.470 kb on + strand, within BT2183 at 2742.471 kb on - strand, within BT2183 at 2742.471 kb on - strand, within BT2183 at 2742.536 kb on + strand, within BT2183 at 2742.537 kb on - strand, within BT2183 at 2742.581 kb on - strand at 2742.679 kb on - strand at 2742.686 kb on + strand at 2742.692 kb on - strand at 2742.713 kb on + strand at 2742.743 kb on - strand at 2742.800 kb on - strand at 2742.849 kb on - strand at 2742.862 kb on - strand at 2742.903 kb on + strand at 2742.925 kb on - strand at 2742.944 kb on - strand, within BT2184 at 2742.947 kb on + strand, within BT2184 at 2743.014 kb on - strand, within BT2184 at 2743.146 kb on + strand, within BT2184 at 2743.147 kb on - strand, within BT2184 at 2743.198 kb on - strand, within BT2184 at 2743.198 kb on - strand, within BT2184 at 2743.198 kb on - strand, within BT2184 at 2743.268 kb on + strand, within BT2184 at 2743.307 kb on - strand, within BT2184 at 2743.400 kb on - strand at 2743.400 kb on - strand at 2743.431 kb on - strand, within BT2185 at 2743.481 kb on - strand, within BT2185 at 2743.481 kb on - strand, within BT2185 at 2743.507 kb on + strand, within BT2185 at 2743.668 kb on + strand, within BT2185 at 2743.668 kb on + strand, within BT2185 at 2743.738 kb on + strand, within BT2185 at 2743.740 kb on + strand, within BT2185 at 2743.741 kb on - strand, within BT2185 at 2743.818 kb on - strand, within BT2185 at 2743.818 kb on - strand, within BT2185 at 2743.893 kb on + strand, within BT2185 at 2743.899 kb on + strand at 2743.902 kb on + strand at 2743.983 kb on + strand at 2743.989 kb on + strand at 2743.989 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Dimetridazole 0.025 mM remove 2,741,885 - BT2183 0.24 -3.9 2,741,901 + BT2183 0.26 -2.2 2,741,902 - BT2183 0.26 -3.6 2,741,903 + BT2183 0.26 -4.6 2,741,904 - BT2183 0.26 -4.4 2,741,945 + BT2183 0.30 -4.3 2,741,945 + BT2183 0.30 -4.5 2,741,946 - BT2183 0.30 -1.0 2,741,998 + BT2183 0.35 -2.3 2,742,160 + BT2183 0.50 -3.7 2,742,237 - BT2183 0.57 -0.6 2,742,237 - BT2183 0.57 -0.6 2,742,377 - BT2183 0.71 -0.2 2,742,470 + BT2183 0.79 -0.6 2,742,471 - BT2183 0.80 -1.0 2,742,471 - BT2183 0.80 -3.8 2,742,536 + BT2183 0.86 +1.0 2,742,537 - BT2183 0.86 +0.0 2,742,581 - -1.0 2,742,679 - -2.7 2,742,686 + -1.8 2,742,692 - -0.3 2,742,713 + -0.6 2,742,743 - +1.6 2,742,800 - -0.8 2,742,849 - +2.3 2,742,862 - +1.0 2,742,903 + +0.5 2,742,925 - +1.0 2,742,944 - BT2184 0.13 +0.0 2,742,947 + BT2184 0.13 -1.3 2,743,014 - BT2184 0.27 +0.3 2,743,146 + BT2184 0.54 -0.6 2,743,147 - BT2184 0.54 +0.2 2,743,198 - BT2184 0.64 +1.7 2,743,198 - BT2184 0.64 +0.7 2,743,198 - BT2184 0.64 +1.4 2,743,268 + BT2184 0.78 +1.0 2,743,307 - BT2184 0.86 -1.3 2,743,400 - -2.1 2,743,400 - -1.0 2,743,431 - BT2185 0.12 +0.0 2,743,481 - BT2185 0.20 -1.2 2,743,481 - BT2185 0.20 -0.6 2,743,507 + BT2185 0.25 +0.0 2,743,668 + BT2185 0.52 +1.0 2,743,668 + BT2185 0.52 -1.7 2,743,738 + BT2185 0.64 -2.0 2,743,740 + BT2185 0.64 -1.7 2,743,741 - BT2185 0.64 -0.8 2,743,818 - BT2185 0.77 +0.0 2,743,818 - BT2185 0.77 -1.3 2,743,893 + BT2185 0.90 -0.7 2,743,899 + -0.7 2,743,902 + -1.0 2,743,983 + +0.1 2,743,989 + -2.7 2,743,989 + +0.0
Or see this region's nucleotide sequence