Experiment: m.b. Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c09090 and PGA1_c09100 overlap by 32 nucleotides PGA1_c09100 and PGA1_c09110 overlap by 4 nucleotides PGA1_c09110 and PGA1_c09120 are separated by 460 nucleotides
PGA1_c09090: PGA1_c09090 - thiamine biosynthesis protein ThiS, at 934,348 to 934,545
_c09090
PGA1_c09100: PGA1_c09100 - putative thiamine biosynthesis oxidoreductase ThiO, at 934,514 to 935,488
_c09100
PGA1_c09110: PGA1_c09110 - thiamine biosynthesis protein ThiC, at 935,485 to 937,284
_c09110
PGA1_c09120: PGA1_c09120 - biotin synthase BioB, at 937,745 to 938,722
_c09120
Position (kb)
935
936
937
938 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 934.513 kb on - strand, within PGA1_c09090 at 934.529 kb on - strand at 934.539 kb on + strand at 934.568 kb on - strand at 934.624 kb on - strand, within PGA1_c09100 at 934.636 kb on - strand, within PGA1_c09100 at 934.659 kb on - strand, within PGA1_c09100 at 934.659 kb on - strand, within PGA1_c09100 at 934.685 kb on + strand, within PGA1_c09100 at 934.685 kb on + strand, within PGA1_c09100 at 934.693 kb on - strand, within PGA1_c09100 at 934.693 kb on - strand, within PGA1_c09100 at 934.693 kb on - strand, within PGA1_c09100 at 934.710 kb on + strand, within PGA1_c09100 at 934.719 kb on - strand, within PGA1_c09100 at 934.846 kb on + strand, within PGA1_c09100 at 934.854 kb on - strand, within PGA1_c09100 at 934.854 kb on - strand, within PGA1_c09100 at 934.903 kb on + strand, within PGA1_c09100 at 934.943 kb on + strand, within PGA1_c09100 at 935.068 kb on - strand, within PGA1_c09100 at 935.081 kb on + strand, within PGA1_c09100 at 935.089 kb on - strand, within PGA1_c09100 at 935.139 kb on + strand, within PGA1_c09100 at 935.147 kb on - strand, within PGA1_c09100 at 935.147 kb on - strand, within PGA1_c09100 at 935.172 kb on + strand, within PGA1_c09100 at 935.177 kb on + strand, within PGA1_c09100 at 935.217 kb on - strand, within PGA1_c09100 at 935.228 kb on - strand, within PGA1_c09100 at 935.276 kb on - strand, within PGA1_c09100 at 935.351 kb on + strand, within PGA1_c09100 at 935.429 kb on - strand at 935.495 kb on + strand at 935.507 kb on + strand at 935.515 kb on - strand at 935.515 kb on - strand at 935.608 kb on + strand at 935.651 kb on - strand at 935.654 kb on + strand at 935.664 kb on + strand at 935.664 kb on + strand at 935.666 kb on + strand, within PGA1_c09110 at 935.682 kb on + strand, within PGA1_c09110 at 935.713 kb on + strand, within PGA1_c09110 at 935.779 kb on + strand, within PGA1_c09110 at 935.779 kb on + strand, within PGA1_c09110 at 935.786 kb on + strand, within PGA1_c09110 at 935.794 kb on - strand, within PGA1_c09110 at 935.950 kb on + strand, within PGA1_c09110 at 935.950 kb on + strand, within PGA1_c09110 at 935.964 kb on - strand, within PGA1_c09110 at 936.044 kb on + strand, within PGA1_c09110 at 936.076 kb on + strand, within PGA1_c09110 at 936.101 kb on - strand, within PGA1_c09110 at 936.107 kb on + strand, within PGA1_c09110 at 936.107 kb on + strand, within PGA1_c09110 at 936.126 kb on + strand, within PGA1_c09110 at 936.142 kb on + strand, within PGA1_c09110 at 936.181 kb on + strand, within PGA1_c09110 at 936.236 kb on - strand, within PGA1_c09110 at 936.349 kb on - strand, within PGA1_c09110 at 936.355 kb on + strand, within PGA1_c09110 at 936.380 kb on - strand, within PGA1_c09110 at 936.407 kb on + strand, within PGA1_c09110 at 936.440 kb on - strand, within PGA1_c09110 at 936.447 kb on - strand, within PGA1_c09110 at 936.565 kb on - strand, within PGA1_c09110 at 936.596 kb on + strand, within PGA1_c09110 at 936.596 kb on + strand, within PGA1_c09110 at 936.596 kb on + strand, within PGA1_c09110 at 936.632 kb on - strand, within PGA1_c09110 at 936.647 kb on + strand, within PGA1_c09110 at 936.664 kb on + strand, within PGA1_c09110 at 936.830 kb on - strand, within PGA1_c09110 at 936.904 kb on + strand, within PGA1_c09110 at 936.906 kb on - strand, within PGA1_c09110 at 936.912 kb on - strand, within PGA1_c09110 at 936.912 kb on - strand, within PGA1_c09110 at 936.913 kb on + strand, within PGA1_c09110 at 936.913 kb on + strand, within PGA1_c09110 at 936.921 kb on - strand, within PGA1_c09110 at 936.937 kb on + strand, within PGA1_c09110 at 937.010 kb on - strand, within PGA1_c09110 at 937.050 kb on + strand, within PGA1_c09110 at 937.079 kb on - strand, within PGA1_c09110 at 937.104 kb on + strand, within PGA1_c09110 at 937.130 kb on - strand at 937.130 kb on - strand at 937.132 kb on + strand at 937.147 kb on - strand at 937.161 kb on + strand at 937.168 kb on - strand at 937.233 kb on + strand at 937.292 kb on - strand at 937.294 kb on + strand at 937.301 kb on + strand at 937.361 kb on + strand at 937.369 kb on - strand at 937.382 kb on + strand at 937.425 kb on + strand at 937.450 kb on + strand at 937.458 kb on - strand at 937.524 kb on - strand at 937.524 kb on - strand at 937.530 kb on + strand at 937.530 kb on + strand at 937.537 kb on + strand at 937.537 kb on + strand at 937.537 kb on + strand at 937.537 kb on + strand at 937.537 kb on + strand at 937.542 kb on + strand at 937.545 kb on - strand at 937.623 kb on + strand at 937.647 kb on + strand at 937.655 kb on + strand at 937.655 kb on + strand at 937.663 kb on - strand at 937.724 kb on - strand at 937.739 kb on - strand at 937.762 kb on + strand at 937.772 kb on + strand at 937.921 kb on + strand, within PGA1_c09120 at 937.929 kb on - strand, within PGA1_c09120 at 937.963 kb on + strand, within PGA1_c09120 at 937.981 kb on + strand, within PGA1_c09120 at 938.067 kb on + strand, within PGA1_c09120 at 938.139 kb on + strand, within PGA1_c09120 at 938.145 kb on + strand, within PGA1_c09120 at 938.145 kb on + strand, within PGA1_c09120 at 938.165 kb on + strand, within PGA1_c09120 at 938.190 kb on - strand, within PGA1_c09120 at 938.241 kb on - strand, within PGA1_c09120 at 938.262 kb on + strand, within PGA1_c09120
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Dimethyl Sulfoxide 7.5 vol% remove 934,513 - PGA1_c09090 0.83 -3.5 934,529 - -1.6 934,539 + -0.4 934,568 - -0.4 934,624 - PGA1_c09100 0.11 -0.6 934,636 - PGA1_c09100 0.13 -2.8 934,659 - PGA1_c09100 0.15 -1.9 934,659 - PGA1_c09100 0.15 -0.6 934,685 + PGA1_c09100 0.18 -3.5 934,685 + PGA1_c09100 0.18 -0.4 934,693 - PGA1_c09100 0.18 -2.1 934,693 - PGA1_c09100 0.18 -0.9 934,693 - PGA1_c09100 0.18 -1.6 934,710 + PGA1_c09100 0.20 -1.4 934,719 - PGA1_c09100 0.21 -2.4 934,846 + PGA1_c09100 0.34 -1.4 934,854 - PGA1_c09100 0.35 -2.7 934,854 - PGA1_c09100 0.35 -0.8 934,903 + PGA1_c09100 0.40 -1.6 934,943 + PGA1_c09100 0.44 -0.3 935,068 - PGA1_c09100 0.57 -1.0 935,081 + PGA1_c09100 0.58 -1.1 935,089 - PGA1_c09100 0.59 -0.6 935,139 + PGA1_c09100 0.64 -1.9 935,147 - PGA1_c09100 0.65 -2.6 935,147 - PGA1_c09100 0.65 -1.2 935,172 + PGA1_c09100 0.67 +0.0 935,177 + PGA1_c09100 0.68 -0.9 935,217 - PGA1_c09100 0.72 -1.4 935,228 - PGA1_c09100 0.73 -1.7 935,276 - PGA1_c09100 0.78 -2.0 935,351 + PGA1_c09100 0.86 -0.8 935,429 - +0.0 935,495 + -2.9 935,507 + -0.6 935,515 - -0.5 935,515 - -1.6 935,608 + -1.0 935,651 - -1.2 935,654 + -1.0 935,664 + +1.8 935,664 + -1.2 935,666 + PGA1_c09110 0.10 +0.1 935,682 + PGA1_c09110 0.11 -0.5 935,713 + PGA1_c09110 0.13 -0.5 935,779 + PGA1_c09110 0.16 -1.1 935,779 + PGA1_c09110 0.16 -1.8 935,786 + PGA1_c09110 0.17 -0.6 935,794 - PGA1_c09110 0.17 -2.8 935,950 + PGA1_c09110 0.26 -0.5 935,950 + PGA1_c09110 0.26 -0.5 935,964 - PGA1_c09110 0.27 -0.6 936,044 + PGA1_c09110 0.31 -2.4 936,076 + PGA1_c09110 0.33 -1.0 936,101 - PGA1_c09110 0.34 +0.0 936,107 + PGA1_c09110 0.35 -1.9 936,107 + PGA1_c09110 0.35 -0.7 936,126 + PGA1_c09110 0.36 -1.7 936,142 + PGA1_c09110 0.36 -0.7 936,181 + PGA1_c09110 0.39 -2.2 936,236 - PGA1_c09110 0.42 -2.7 936,349 - PGA1_c09110 0.48 -1.4 936,355 + PGA1_c09110 0.48 -1.6 936,380 - PGA1_c09110 0.50 -0.1 936,407 + PGA1_c09110 0.51 -2.4 936,440 - PGA1_c09110 0.53 -1.5 936,447 - PGA1_c09110 0.53 -2.0 936,565 - PGA1_c09110 0.60 -1.4 936,596 + PGA1_c09110 0.62 -1.4 936,596 + PGA1_c09110 0.62 +0.6 936,596 + PGA1_c09110 0.62 -1.9 936,632 - PGA1_c09110 0.64 -1.0 936,647 + PGA1_c09110 0.65 -2.2 936,664 + PGA1_c09110 0.66 -0.4 936,830 - PGA1_c09110 0.75 +0.2 936,904 + PGA1_c09110 0.79 -1.6 936,906 - PGA1_c09110 0.79 -1.6 936,912 - PGA1_c09110 0.79 -1.2 936,912 - PGA1_c09110 0.79 -2.6 936,913 + PGA1_c09110 0.79 -2.5 936,913 + PGA1_c09110 0.79 +0.4 936,921 - PGA1_c09110 0.80 -1.4 936,937 + PGA1_c09110 0.81 -1.4 937,010 - PGA1_c09110 0.85 -2.0 937,050 + PGA1_c09110 0.87 -1.0 937,079 - PGA1_c09110 0.89 -0.1 937,104 + PGA1_c09110 0.90 -1.2 937,130 - -0.4 937,130 - -2.5 937,132 + -0.7 937,147 - -2.0 937,161 + -0.5 937,168 - -0.6 937,233 + -1.4 937,292 - -0.4 937,294 + -1.5 937,301 + -0.4 937,361 + -2.0 937,369 - -2.4 937,382 + -1.2 937,425 + -2.6 937,450 + -2.0 937,458 - -1.2 937,524 - -1.9 937,524 - -1.2 937,530 + -1.1 937,530 + -0.2 937,537 + -1.6 937,537 + -1.8 937,537 + -3.4 937,537 + -0.4 937,537 + -2.0 937,542 + -0.3 937,545 - -1.0 937,623 + -0.1 937,647 + -0.4 937,655 + -1.8 937,655 + +0.4 937,663 - +0.3 937,724 - +0.5 937,739 - -0.3 937,762 + -0.1 937,772 + +1.4 937,921 + PGA1_c09120 0.18 -0.8 937,929 - PGA1_c09120 0.19 +1.3 937,963 + PGA1_c09120 0.22 +0.6 937,981 + PGA1_c09120 0.24 -0.2 938,067 + PGA1_c09120 0.33 +1.5 938,139 + PGA1_c09120 0.40 -0.6 938,145 + PGA1_c09120 0.41 -0.2 938,145 + PGA1_c09120 0.41 +0.5 938,165 + PGA1_c09120 0.43 +0.6 938,190 - PGA1_c09120 0.46 +0.6 938,241 - PGA1_c09120 0.51 -1.2 938,262 + PGA1_c09120 0.53 -1.4
Or see this region's nucleotide sequence