Experiment: m.b. Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_65p00300 and PGA1_65p00310 are separated by 4 nucleotides PGA1_65p00310 and PGA1_65p00320 are separated by 190 nucleotides
PGA1_65p00300: PGA1_65p00300 - GDP-L-fucose synthetase Fcl, at 39,409 to 40,398
_65p00300
PGA1_65p00310: PGA1_65p00310 - GDP-mannose 4,6-dehydratase Gmd, at 40,403 to 41,521
_65p00310
PGA1_65p00320: PGA1_65p00320 - type I secretion membrane fusion protein, at 41,712 to 43,019
_65p00320
Position (kb) 40
41
42 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 39.457 kb on + strand at 39.465 kb on - strand at 39.586 kb on + strand, within PGA1_65p00300 at 39.594 kb on - strand, within PGA1_65p00300 at 39.939 kb on + strand, within PGA1_65p00300 at 39.979 kb on - strand, within PGA1_65p00300 at 40.103 kb on + strand, within PGA1_65p00300 at 40.343 kb on - strand at 40.501 kb on + strand at 40.804 kb on + strand, within PGA1_65p00310 at 40.883 kb on + strand, within PGA1_65p00310 at 40.893 kb on - strand, within PGA1_65p00310 at 40.901 kb on - strand, within PGA1_65p00310 at 41.119 kb on + strand, within PGA1_65p00310 at 41.119 kb on + strand, within PGA1_65p00310 at 41.322 kb on - strand, within PGA1_65p00310 at 41.537 kb on - strand at 41.556 kb on + strand at 41.556 kb on + strand at 41.564 kb on - strand at 41.564 kb on - strand at 41.585 kb on - strand at 41.600 kb on + strand at 41.889 kb on - strand, within PGA1_65p00320 at 41.977 kb on - strand, within PGA1_65p00320
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Dimethyl Sulfoxide 7.5 vol% remove 39,457 + -0.6 39,465 - -0.1 39,586 + PGA1_65p00300 0.18 -0.8 39,594 - PGA1_65p00300 0.19 +0.6 39,939 + PGA1_65p00300 0.54 +1.1 39,979 - PGA1_65p00300 0.58 -3.6 40,103 + PGA1_65p00300 0.70 -0.2 40,343 - -1.0 40,501 + -0.2 40,804 + PGA1_65p00310 0.36 -1.5 40,883 + PGA1_65p00310 0.43 -0.2 40,893 - PGA1_65p00310 0.44 +0.2 40,901 - PGA1_65p00310 0.45 -0.2 41,119 + PGA1_65p00310 0.64 -0.6 41,119 + PGA1_65p00310 0.64 -1.1 41,322 - PGA1_65p00310 0.82 -1.2 41,537 - -1.2 41,556 + -0.2 41,556 + +0.8 41,564 - -0.6 41,564 - -0.7 41,585 - +0.3 41,600 + -1.2 41,889 - PGA1_65p00320 0.14 -1.2 41,977 - PGA1_65p00320 0.20 -0.6
Or see this region's nucleotide sequence