Experiment: m.b. Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_78p00190 and PGA1_78p00200 are separated by 20 nucleotides PGA1_78p00200 and PGA1_78p00210 are separated by 9 nucleotides PGA1_78p00210 and PGA1_78p00220 are separated by 49 nucleotides
PGA1_78p00190: PGA1_78p00190 - sn-glycerol-3-phosphate import ATP-binding protein UgbC, at 22,202 to 23,287
_78p00190
PGA1_78p00200: PGA1_78p00200 - Sugar phosphate isomerases/epimerases, at 23,308 to 24,051
_78p00200
PGA1_78p00210: PGA1_78p00210 - putative oxidoreductase, at 24,061 to 25,194
_78p00210
PGA1_78p00220: PGA1_78p00220 - transcriptional regulatory protein, LysR family, at 25,244 to 26,131
_78p00220
Position (kb) 24
25
26 Strain fitness (log2 ratio) -2
-1
0
1 at 23.154 kb on - strand, within PGA1_78p00190 at 23.781 kb on - strand, within PGA1_78p00200 at 23.786 kb on - strand, within PGA1_78p00200 at 24.221 kb on + strand, within PGA1_78p00210 at 24.532 kb on - strand, within PGA1_78p00210 at 24.597 kb on + strand, within PGA1_78p00210 at 24.853 kb on - strand, within PGA1_78p00210 at 25.032 kb on + strand, within PGA1_78p00210 at 25.362 kb on + strand, within PGA1_78p00220 at 25.560 kb on - strand, within PGA1_78p00220 at 26.110 kb on + strand at 26.118 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Dimethyl Sulfoxide 7.5 vol% remove 23,154 - PGA1_78p00190 0.88 +0.4 23,781 - PGA1_78p00200 0.64 +0.4 23,786 - PGA1_78p00200 0.64 -1.5 24,221 + PGA1_78p00210 0.14 +0.7 24,532 - PGA1_78p00210 0.42 -1.0 24,597 + PGA1_78p00210 0.47 -2.3 24,853 - PGA1_78p00210 0.70 -0.5 25,032 + PGA1_78p00210 0.86 -0.1 25,362 + PGA1_78p00220 0.13 -0.7 25,560 - PGA1_78p00220 0.36 -0.1 26,110 + -0.1 26,118 - -0.0
Or see this region's nucleotide sequence