Experiment: m.b. Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c03350 and PGA1_c03360 are separated by 269 nucleotides PGA1_c03360 and PGA1_c03365 are separated by 156 nucleotides PGA1_c03365 and PGA1_c03370 are separated by 163 nucleotides PGA1_c03370 and PGA1_c03380 are separated by 109 nucleotides
PGA1_c03350: PGA1_c03350 - octaprenyl-diphosphate synthase IspB, at 321,425 to 322,423
_c03350
PGA1_c03360: PGA1_c03360 - Predicted O-methyltransferase, at 322,693 to 323,460
_c03360
PGA1_c03365: PGA1_c03365 - Uncharacterized small protein, at 323,617 to 323,796
_c03365
PGA1_c03370: PGA1_c03370 - hypothetical protein, at 323,960 to 324,166
_c03370
PGA1_c03380: PGA1_c03380 - putative glycine cleavage system transcriptional activator, at 324,276 to 325,157
_c03380
Position (kb) 322
323
324 Strain fitness (log2 ratio) -2
-1
0
1
2 at 322.448 kb on - strand at 322.620 kb on + strand at 322.627 kb on + strand at 322.628 kb on - strand at 322.643 kb on - strand at 322.649 kb on + strand at 322.700 kb on + strand at 323.059 kb on + strand, within PGA1_c03360 at 323.059 kb on + strand, within PGA1_c03360 at 323.082 kb on - strand, within PGA1_c03360 at 323.092 kb on - strand, within PGA1_c03360 at 323.092 kb on - strand, within PGA1_c03360 at 323.092 kb on - strand, within PGA1_c03360 at 323.242 kb on - strand, within PGA1_c03360 at 323.440 kb on + strand at 323.445 kb on + strand at 323.445 kb on + strand at 323.445 kb on + strand at 323.445 kb on + strand at 323.504 kb on - strand at 323.635 kb on - strand, within PGA1_c03365 at 323.685 kb on + strand, within PGA1_c03365 at 323.702 kb on + strand, within PGA1_c03365 at 323.777 kb on - strand, within PGA1_c03365 at 323.777 kb on - strand, within PGA1_c03365 at 323.777 kb on - strand, within PGA1_c03365 at 323.813 kb on + strand at 323.847 kb on - strand at 323.847 kb on - strand at 323.868 kb on + strand at 323.915 kb on - strand at 323.923 kb on + strand at 324.017 kb on + strand, within PGA1_c03370 at 324.017 kb on + strand, within PGA1_c03370 at 324.024 kb on + strand, within PGA1_c03370 at 324.087 kb on - strand, within PGA1_c03370 at 324.120 kb on + strand, within PGA1_c03370 at 324.128 kb on + strand, within PGA1_c03370 at 324.128 kb on + strand, within PGA1_c03370 at 324.199 kb on - strand at 324.222 kb on + strand at 324.227 kb on + strand at 324.232 kb on + strand at 324.235 kb on - strand at 324.235 kb on - strand at 324.235 kb on - strand at 324.235 kb on - strand at 324.240 kb on - strand at 324.277 kb on + strand at 324.278 kb on + strand at 324.278 kb on + strand at 324.278 kb on + strand at 324.286 kb on - strand at 324.286 kb on - strand at 324.286 kb on - strand at 324.339 kb on - strand at 324.370 kb on + strand, within PGA1_c03380 at 324.420 kb on - strand, within PGA1_c03380 at 324.434 kb on - strand, within PGA1_c03380
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Dimethyl Sulfoxide 7.5 vol% remove 322,448 - -1.8 322,620 + +0.2 322,627 + -1.0 322,628 - -0.4 322,643 - +0.8 322,649 + -0.2 322,700 + -1.5 323,059 + PGA1_c03360 0.48 -1.7 323,059 + PGA1_c03360 0.48 -0.8 323,082 - PGA1_c03360 0.51 -0.5 323,092 - PGA1_c03360 0.52 -1.2 323,092 - PGA1_c03360 0.52 -2.2 323,092 - PGA1_c03360 0.52 +0.1 323,242 - PGA1_c03360 0.71 -0.8 323,440 + -0.5 323,445 + -0.5 323,445 + +2.2 323,445 + +0.1 323,445 + -0.5 323,504 - +0.3 323,635 - PGA1_c03365 0.10 -1.6 323,685 + PGA1_c03365 0.38 +0.5 323,702 + PGA1_c03365 0.47 -0.7 323,777 - PGA1_c03365 0.89 -0.2 323,777 - PGA1_c03365 0.89 -0.4 323,777 - PGA1_c03365 0.89 +1.1 323,813 + -0.2 323,847 - -0.1 323,847 - +0.4 323,868 + -0.5 323,915 - -0.2 323,923 + +0.2 324,017 + PGA1_c03370 0.28 -1.0 324,017 + PGA1_c03370 0.28 +0.8 324,024 + PGA1_c03370 0.31 -0.2 324,087 - PGA1_c03370 0.61 +0.9 324,120 + PGA1_c03370 0.77 -0.4 324,128 + PGA1_c03370 0.81 -0.6 324,128 + PGA1_c03370 0.81 -0.1 324,199 - +0.2 324,222 + -0.3 324,227 + -1.2 324,232 + +1.0 324,235 - -2.2 324,235 - -0.6 324,235 - +0.3 324,235 - -0.2 324,240 - -0.0 324,277 + +1.0 324,278 + +0.1 324,278 + -0.3 324,278 + -0.2 324,286 - +0.4 324,286 - -0.7 324,286 - +0.8 324,339 - +0.4 324,370 + PGA1_c03380 0.11 -0.3 324,420 - PGA1_c03380 0.16 +0.8 324,434 - PGA1_c03380 0.18 +0.5
Or see this region's nucleotide sequence