Experiment: m.b. Dimethyl Sulfoxide 7.5 vol%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c26450 and PGA1_c26460 are separated by 152 nucleotides PGA1_c26460 and PGA1_c26470 are separated by 278 nucleotides
PGA1_c26450: PGA1_c26450 - glutamine-dependent NAD(+) synthetase NadE, at 2,755,629 to 2,757,287
_c26450
PGA1_c26460: PGA1_c26460 - Uncharacterized protein conserved in bacteria, at 2,757,440 to 2,758,972
_c26460
PGA1_c26470: PGA1_c26470 - 2-isopropylmalate synthase LeuA, at 2,759,251 to 2,760,822
_c26470
Position (kb)
2757
2758
2759 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2757.353 kb on - strand at 2757.358 kb on - strand at 2757.394 kb on + strand at 2757.418 kb on + strand at 2757.418 kb on + strand at 2757.418 kb on + strand at 2757.426 kb on - strand at 2757.436 kb on + strand at 2757.482 kb on + strand at 2757.482 kb on + strand at 2757.482 kb on + strand at 2757.482 kb on + strand at 2757.490 kb on - strand at 2757.497 kb on - strand at 2757.541 kb on + strand at 2757.628 kb on + strand, within PGA1_c26460 at 2757.630 kb on + strand, within PGA1_c26460 at 2757.630 kb on + strand, within PGA1_c26460 at 2757.630 kb on + strand, within PGA1_c26460 at 2757.630 kb on + strand, within PGA1_c26460 at 2757.630 kb on + strand, within PGA1_c26460 at 2757.630 kb on + strand, within PGA1_c26460 at 2757.630 kb on + strand, within PGA1_c26460 at 2757.638 kb on - strand, within PGA1_c26460 at 2757.638 kb on - strand, within PGA1_c26460 at 2757.640 kb on + strand, within PGA1_c26460 at 2757.648 kb on - strand, within PGA1_c26460 at 2757.720 kb on + strand, within PGA1_c26460 at 2757.895 kb on + strand, within PGA1_c26460 at 2757.903 kb on - strand, within PGA1_c26460 at 2757.903 kb on - strand, within PGA1_c26460 at 2757.953 kb on - strand, within PGA1_c26460 at 2758.022 kb on + strand, within PGA1_c26460 at 2758.074 kb on - strand, within PGA1_c26460 at 2758.108 kb on - strand, within PGA1_c26460 at 2758.112 kb on - strand, within PGA1_c26460 at 2758.123 kb on + strand, within PGA1_c26460 at 2758.169 kb on - strand, within PGA1_c26460 at 2758.171 kb on + strand, within PGA1_c26460 at 2758.172 kb on + strand, within PGA1_c26460 at 2758.229 kb on + strand, within PGA1_c26460 at 2758.242 kb on - strand, within PGA1_c26460 at 2758.276 kb on - strand, within PGA1_c26460 at 2758.310 kb on + strand, within PGA1_c26460 at 2758.318 kb on - strand, within PGA1_c26460 at 2758.475 kb on + strand, within PGA1_c26460 at 2758.480 kb on + strand, within PGA1_c26460 at 2758.480 kb on + strand, within PGA1_c26460 at 2758.480 kb on + strand, within PGA1_c26460 at 2758.480 kb on + strand, within PGA1_c26460 at 2758.484 kb on - strand, within PGA1_c26460 at 2758.488 kb on - strand, within PGA1_c26460 at 2758.488 kb on - strand, within PGA1_c26460 at 2758.488 kb on - strand, within PGA1_c26460 at 2758.488 kb on - strand, within PGA1_c26460 at 2758.488 kb on - strand, within PGA1_c26460 at 2758.518 kb on - strand, within PGA1_c26460 at 2758.525 kb on - strand, within PGA1_c26460 at 2758.600 kb on + strand, within PGA1_c26460 at 2758.608 kb on - strand, within PGA1_c26460 at 2758.622 kb on - strand, within PGA1_c26460 at 2758.627 kb on - strand, within PGA1_c26460 at 2758.720 kb on - strand, within PGA1_c26460 at 2758.733 kb on + strand, within PGA1_c26460 at 2758.733 kb on + strand, within PGA1_c26460 at 2758.782 kb on - strand, within PGA1_c26460 at 2758.794 kb on + strand, within PGA1_c26460 at 2758.795 kb on - strand, within PGA1_c26460 at 2758.882 kb on + strand at 2758.904 kb on + strand at 2758.912 kb on - strand at 2758.912 kb on - strand at 2758.914 kb on + strand at 2758.927 kb on - strand at 2759.061 kb on + strand at 2759.062 kb on - strand at 2759.064 kb on + strand at 2759.066 kb on + strand at 2759.066 kb on + strand at 2759.072 kb on - strand at 2759.072 kb on - strand at 2759.072 kb on - strand at 2759.074 kb on - strand at 2759.074 kb on - strand at 2759.074 kb on - strand at 2759.074 kb on - strand at 2759.074 kb on - strand at 2759.089 kb on - strand at 2759.127 kb on + strand at 2759.150 kb on - strand at 2759.161 kb on + strand at 2759.195 kb on + strand at 2759.195 kb on + strand at 2759.250 kb on + strand at 2759.329 kb on + strand at 2759.329 kb on + strand at 2759.329 kb on + strand at 2759.357 kb on - strand at 2759.381 kb on + strand at 2759.405 kb on + strand at 2759.452 kb on - strand, within PGA1_c26470 at 2759.481 kb on + strand, within PGA1_c26470 at 2759.503 kb on + strand, within PGA1_c26470 at 2759.550 kb on + strand, within PGA1_c26470 at 2759.583 kb on - strand, within PGA1_c26470 at 2759.583 kb on - strand, within PGA1_c26470 at 2759.599 kb on - strand, within PGA1_c26470 at 2759.607 kb on + strand, within PGA1_c26470 at 2759.615 kb on - strand, within PGA1_c26470 at 2759.648 kb on + strand, within PGA1_c26470 at 2759.668 kb on + strand, within PGA1_c26470 at 2759.706 kb on + strand, within PGA1_c26470 at 2759.714 kb on - strand, within PGA1_c26470 at 2759.778 kb on + strand, within PGA1_c26470 at 2759.791 kb on - strand, within PGA1_c26470 at 2759.791 kb on - strand, within PGA1_c26470 at 2759.809 kb on - strand, within PGA1_c26470 at 2759.920 kb on + strand, within PGA1_c26470 at 2759.924 kb on + strand, within PGA1_c26470 at 2759.953 kb on - strand, within PGA1_c26470
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Dimethyl Sulfoxide 7.5 vol% remove 2,757,353 - -0.6 2,757,358 - +1.1 2,757,394 + +0.4 2,757,418 + +0.0 2,757,418 + +0.1 2,757,418 + +0.5 2,757,426 - -2.0 2,757,436 + +0.4 2,757,482 + +1.8 2,757,482 + -1.0 2,757,482 + -1.5 2,757,482 + -0.4 2,757,490 - -0.1 2,757,497 - -0.1 2,757,541 + -1.1 2,757,628 + PGA1_c26460 0.12 +0.6 2,757,630 + PGA1_c26460 0.12 +0.2 2,757,630 + PGA1_c26460 0.12 -0.7 2,757,630 + PGA1_c26460 0.12 -1.0 2,757,630 + PGA1_c26460 0.12 +0.2 2,757,630 + PGA1_c26460 0.12 +0.3 2,757,630 + PGA1_c26460 0.12 -2.0 2,757,630 + PGA1_c26460 0.12 -1.0 2,757,638 - PGA1_c26460 0.13 +1.4 2,757,638 - PGA1_c26460 0.13 -1.2 2,757,640 + PGA1_c26460 0.13 +0.6 2,757,648 - PGA1_c26460 0.14 -0.2 2,757,720 + PGA1_c26460 0.18 +0.6 2,757,895 + PGA1_c26460 0.30 -2.7 2,757,903 - PGA1_c26460 0.30 +0.2 2,757,903 - PGA1_c26460 0.30 -0.7 2,757,953 - PGA1_c26460 0.33 -1.3 2,758,022 + PGA1_c26460 0.38 -0.1 2,758,074 - PGA1_c26460 0.41 -1.4 2,758,108 - PGA1_c26460 0.44 -0.7 2,758,112 - PGA1_c26460 0.44 -1.0 2,758,123 + PGA1_c26460 0.45 -1.1 2,758,169 - PGA1_c26460 0.48 -0.4 2,758,171 + PGA1_c26460 0.48 -1.0 2,758,172 + PGA1_c26460 0.48 -0.6 2,758,229 + PGA1_c26460 0.51 -0.4 2,758,242 - PGA1_c26460 0.52 -0.8 2,758,276 - PGA1_c26460 0.55 -1.8 2,758,310 + PGA1_c26460 0.57 -0.4 2,758,318 - PGA1_c26460 0.57 -1.8 2,758,475 + PGA1_c26460 0.68 -0.8 2,758,480 + PGA1_c26460 0.68 +0.1 2,758,480 + PGA1_c26460 0.68 -0.6 2,758,480 + PGA1_c26460 0.68 -0.2 2,758,480 + PGA1_c26460 0.68 -0.7 2,758,484 - PGA1_c26460 0.68 +0.3 2,758,488 - PGA1_c26460 0.68 +0.4 2,758,488 - PGA1_c26460 0.68 -0.3 2,758,488 - PGA1_c26460 0.68 +0.3 2,758,488 - PGA1_c26460 0.68 +0.6 2,758,488 - PGA1_c26460 0.68 -1.2 2,758,518 - PGA1_c26460 0.70 -0.8 2,758,525 - PGA1_c26460 0.71 +0.6 2,758,600 + PGA1_c26460 0.76 -1.8 2,758,608 - PGA1_c26460 0.76 -0.0 2,758,622 - PGA1_c26460 0.77 -0.3 2,758,627 - PGA1_c26460 0.77 -0.7 2,758,720 - PGA1_c26460 0.83 -0.4 2,758,733 + PGA1_c26460 0.84 -1.5 2,758,733 + PGA1_c26460 0.84 +0.4 2,758,782 - PGA1_c26460 0.88 +0.4 2,758,794 + PGA1_c26460 0.88 -0.2 2,758,795 - PGA1_c26460 0.88 +0.0 2,758,882 + +0.1 2,758,904 + +1.0 2,758,912 - -1.7 2,758,912 - -0.5 2,758,914 + -1.4 2,758,927 - +0.6 2,759,061 + +1.5 2,759,062 - -0.5 2,759,064 + -1.2 2,759,066 + -2.0 2,759,066 + -0.4 2,759,072 - -1.4 2,759,072 - +0.2 2,759,072 - -0.4 2,759,074 - -1.0 2,759,074 - +0.6 2,759,074 - +0.1 2,759,074 - -1.0 2,759,074 - -0.1 2,759,089 - +0.0 2,759,127 + +0.6 2,759,150 - -0.8 2,759,161 + +0.5 2,759,195 + -0.0 2,759,195 + -0.4 2,759,250 + +0.7 2,759,329 + +1.3 2,759,329 + -0.3 2,759,329 + +0.6 2,759,357 - -0.6 2,759,381 + -0.8 2,759,405 + -0.0 2,759,452 - PGA1_c26470 0.13 +0.9 2,759,481 + PGA1_c26470 0.15 +0.9 2,759,503 + PGA1_c26470 0.16 +2.2 2,759,550 + PGA1_c26470 0.19 -0.4 2,759,583 - PGA1_c26470 0.21 +0.2 2,759,583 - PGA1_c26470 0.21 -0.2 2,759,599 - PGA1_c26470 0.22 -2.4 2,759,607 + PGA1_c26470 0.23 +1.2 2,759,615 - PGA1_c26470 0.23 +0.2 2,759,648 + PGA1_c26470 0.25 +0.5 2,759,668 + PGA1_c26470 0.27 -0.4 2,759,706 + PGA1_c26470 0.29 -0.0 2,759,714 - PGA1_c26470 0.29 -0.8 2,759,778 + PGA1_c26470 0.34 +0.3 2,759,791 - PGA1_c26470 0.34 -0.0 2,759,791 - PGA1_c26470 0.34 +0.3 2,759,809 - PGA1_c26470 0.35 -0.4 2,759,920 + PGA1_c26470 0.43 +0.0 2,759,924 + PGA1_c26470 0.43 -1.0 2,759,953 - PGA1_c26470 0.45 +1.1
Or see this region's nucleotide sequence