Experiment: PYE 0.5x 30C survival rep3 12 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_00052 and CCNA_00053 are separated by 37 nucleotides CCNA_00053 and CCNA_00054 overlap by 4 nucleotides CCNA_00054 and CCNA_00055 are separated by 189 nucleotides CCNA_00055 and CCNA_00056 are separated by 45 nucleotides
CCNA_00052: CCNA_00052 - metal-dependent hydrolase/metalloproteinase, at 55,231 to 55,686
_00052
CCNA_00053: CCNA_00053 - phosphate starvation-inducible protein PhoH, at 55,724 to 56,668
_00053
CCNA_00054: CCNA_00054 - tRNA 2-methylthioadenosine synthase, at 56,665 to 58,011
_00054
CCNA_00055: CCNA_00055 - ferric uptake regulation protein, at 58,201 to 58,608
_00055
CCNA_00056: CCNA_00056 - ribosomal-protein-S18-alanine acetyltransferase, at 58,654 to 59,106
_00056
Position (kb) 56
57
58
59 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 55.669 kb on - strand at 55.679 kb on + strand at 55.696 kb on + strand at 55.720 kb on + strand at 55.736 kb on + strand at 55.744 kb on - strand at 55.768 kb on - strand at 55.824 kb on + strand, within CCNA_00053 at 55.830 kb on + strand, within CCNA_00053 at 55.838 kb on - strand, within CCNA_00053 at 55.838 kb on - strand, within CCNA_00053 at 55.838 kb on - strand, within CCNA_00053 at 55.855 kb on - strand, within CCNA_00053 at 55.862 kb on + strand, within CCNA_00053 at 55.874 kb on - strand, within CCNA_00053 at 55.882 kb on + strand, within CCNA_00053 at 55.899 kb on - strand, within CCNA_00053 at 55.946 kb on + strand, within CCNA_00053 at 55.959 kb on - strand, within CCNA_00053 at 55.964 kb on - strand, within CCNA_00053 at 55.979 kb on + strand, within CCNA_00053 at 55.979 kb on + strand, within CCNA_00053 at 55.979 kb on + strand, within CCNA_00053 at 55.987 kb on - strand, within CCNA_00053 at 55.987 kb on - strand, within CCNA_00053 at 55.987 kb on - strand, within CCNA_00053 at 55.987 kb on - strand, within CCNA_00053 at 55.987 kb on - strand, within CCNA_00053 at 55.987 kb on - strand, within CCNA_00053 at 55.987 kb on - strand, within CCNA_00053 at 56.007 kb on - strand, within CCNA_00053 at 56.025 kb on - strand, within CCNA_00053 at 56.025 kb on - strand, within CCNA_00053 at 56.049 kb on + strand, within CCNA_00053 at 56.049 kb on + strand, within CCNA_00053 at 56.050 kb on + strand, within CCNA_00053 at 56.089 kb on - strand, within CCNA_00053 at 56.089 kb on - strand, within CCNA_00053 at 56.186 kb on + strand, within CCNA_00053 at 56.209 kb on + strand, within CCNA_00053 at 56.250 kb on - strand, within CCNA_00053 at 56.270 kb on + strand, within CCNA_00053 at 56.288 kb on - strand, within CCNA_00053 at 56.299 kb on - strand, within CCNA_00053 at 56.319 kb on - strand, within CCNA_00053 at 56.327 kb on + strand, within CCNA_00053 at 56.334 kb on - strand, within CCNA_00053 at 56.357 kb on + strand, within CCNA_00053 at 56.364 kb on + strand, within CCNA_00053 at 56.372 kb on - strand, within CCNA_00053 at 56.390 kb on - strand, within CCNA_00053 at 56.405 kb on + strand, within CCNA_00053 at 56.418 kb on - strand, within CCNA_00053 at 56.432 kb on + strand, within CCNA_00053 at 56.450 kb on - strand, within CCNA_00053 at 56.474 kb on + strand, within CCNA_00053 at 56.484 kb on - strand, within CCNA_00053 at 56.491 kb on - strand, within CCNA_00053 at 56.502 kb on + strand, within CCNA_00053 at 56.533 kb on + strand, within CCNA_00053 at 56.566 kb on + strand, within CCNA_00053 at 56.566 kb on + strand, within CCNA_00053 at 56.574 kb on - strand at 56.574 kb on - strand at 56.577 kb on + strand at 56.577 kb on + strand at 56.582 kb on + strand at 56.618 kb on + strand at 56.618 kb on + strand at 56.639 kb on - strand at 56.648 kb on + strand at 56.651 kb on - strand at 56.656 kb on - strand at 56.656 kb on - strand at 56.658 kb on - strand at 56.674 kb on + strand at 56.682 kb on - strand at 56.684 kb on + strand at 56.687 kb on - strand at 56.692 kb on - strand at 56.692 kb on - strand at 56.707 kb on + strand at 56.715 kb on - strand at 56.734 kb on + strand at 56.780 kb on + strand at 56.795 kb on - strand at 56.819 kb on - strand, within CCNA_00054 at 56.860 kb on + strand, within CCNA_00054 at 56.912 kb on - strand, within CCNA_00054 at 56.912 kb on - strand, within CCNA_00054 at 56.927 kb on + strand, within CCNA_00054 at 56.927 kb on + strand, within CCNA_00054 at 56.935 kb on - strand, within CCNA_00054 at 56.935 kb on - strand, within CCNA_00054 at 56.935 kb on - strand, within CCNA_00054 at 57.034 kb on + strand, within CCNA_00054 at 57.041 kb on - strand, within CCNA_00054 at 57.041 kb on - strand, within CCNA_00054 at 57.069 kb on - strand, within CCNA_00054 at 57.080 kb on + strand, within CCNA_00054 at 57.138 kb on + strand, within CCNA_00054 at 57.138 kb on + strand, within CCNA_00054 at 57.146 kb on - strand, within CCNA_00054 at 57.148 kb on + strand, within CCNA_00054 at 57.148 kb on + strand, within CCNA_00054 at 57.148 kb on + strand, within CCNA_00054 at 57.156 kb on - strand, within CCNA_00054 at 57.156 kb on - strand, within CCNA_00054 at 57.156 kb on - strand, within CCNA_00054 at 57.156 kb on - strand, within CCNA_00054 at 57.156 kb on - strand, within CCNA_00054 at 57.156 kb on - strand, within CCNA_00054 at 57.169 kb on + strand, within CCNA_00054 at 57.169 kb on + strand, within CCNA_00054 at 57.177 kb on - strand, within CCNA_00054 at 57.177 kb on - strand, within CCNA_00054 at 57.177 kb on - strand, within CCNA_00054 at 57.194 kb on + strand, within CCNA_00054 at 57.256 kb on + strand, within CCNA_00054 at 57.307 kb on + strand, within CCNA_00054 at 57.315 kb on - strand, within CCNA_00054 at 57.320 kb on + strand, within CCNA_00054 at 57.352 kb on + strand, within CCNA_00054 at 57.352 kb on + strand, within CCNA_00054 at 57.375 kb on - strand, within CCNA_00054 at 57.387 kb on + strand, within CCNA_00054 at 57.395 kb on - strand, within CCNA_00054 at 57.412 kb on + strand, within CCNA_00054 at 57.419 kb on - strand, within CCNA_00054 at 57.419 kb on - strand, within CCNA_00054 at 57.428 kb on + strand, within CCNA_00054 at 57.428 kb on + strand, within CCNA_00054 at 57.487 kb on + strand, within CCNA_00054 at 57.492 kb on - strand, within CCNA_00054 at 57.495 kb on - strand, within CCNA_00054 at 57.511 kb on - strand, within CCNA_00054 at 57.511 kb on - strand, within CCNA_00054 at 57.531 kb on - strand, within CCNA_00054 at 57.588 kb on - strand, within CCNA_00054 at 57.623 kb on - strand, within CCNA_00054 at 57.665 kb on + strand, within CCNA_00054 at 57.733 kb on - strand, within CCNA_00054 at 57.738 kb on - strand, within CCNA_00054 at 57.768 kb on - strand, within CCNA_00054 at 57.816 kb on + strand, within CCNA_00054 at 57.829 kb on - strand, within CCNA_00054 at 57.829 kb on - strand, within CCNA_00054 at 57.852 kb on + strand, within CCNA_00054 at 57.868 kb on - strand, within CCNA_00054 at 57.868 kb on - strand, within CCNA_00054 at 57.870 kb on - strand, within CCNA_00054 at 57.892 kb on - strand at 57.910 kb on + strand at 57.913 kb on - strand at 57.932 kb on + strand at 57.940 kb on - strand at 57.946 kb on + strand at 57.954 kb on - strand at 58.001 kb on + strand at 58.027 kb on - strand at 58.035 kb on + strand at 58.043 kb on + strand at 58.043 kb on + strand at 58.043 kb on - strand at 58.051 kb on - strand at 58.058 kb on - strand at 58.092 kb on + strand at 58.111 kb on + strand at 58.111 kb on + strand at 58.116 kb on + strand at 58.116 kb on + strand at 58.124 kb on - strand at 58.124 kb on - strand at 58.178 kb on - strand at 58.179 kb on - strand at 58.192 kb on + strand at 58.192 kb on - strand at 58.192 kb on - strand at 58.623 kb on - strand at 58.651 kb on - strand at 58.675 kb on + strand at 58.709 kb on + strand, within CCNA_00056 at 58.733 kb on + strand, within CCNA_00056 at 58.742 kb on - strand, within CCNA_00056 at 58.751 kb on + strand, within CCNA_00056 at 58.751 kb on + strand, within CCNA_00056 at 58.759 kb on - strand, within CCNA_00056 at 58.759 kb on - strand at 58.759 kb on - strand, within CCNA_00056 at 58.764 kb on - strand, within CCNA_00056 at 58.811 kb on + strand, within CCNA_00056 at 58.819 kb on - strand, within CCNA_00056 at 58.819 kb on - strand, within CCNA_00056 at 58.819 kb on - strand, within CCNA_00056 at 58.841 kb on + strand, within CCNA_00056 at 58.849 kb on - strand, within CCNA_00056 at 58.854 kb on - strand, within CCNA_00056 at 58.854 kb on - strand, within CCNA_00056 at 58.854 kb on - strand, within CCNA_00056 at 58.943 kb on - strand, within CCNA_00056 at 58.943 kb on - strand, within CCNA_00056 at 58.943 kb on - strand, within CCNA_00056 at 58.943 kb on - strand, within CCNA_00056 at 58.951 kb on - strand, within CCNA_00056
Per-strain Table
Position Strand Gene LocusTag Fraction PYE 0.5x 30C survival rep3 12 days remove 55,669 - -0.8 55,679 + -0.1 55,696 + -1.2 55,720 + +1.1 55,736 + +0.3 55,744 - +1.5 55,768 - +1.0 55,824 + CCNA_00053 0.11 -1.3 55,830 + CCNA_00053 0.11 +0.4 55,838 - CCNA_00053 0.12 +1.1 55,838 - CCNA_00053 0.12 +1.5 55,838 - CCNA_00053 0.12 +1.4 55,855 - CCNA_00053 0.14 +1.2 55,862 + CCNA_00053 0.15 +0.8 55,874 - CCNA_00053 0.16 +1.6 55,882 + CCNA_00053 0.17 -1.3 55,899 - CCNA_00053 0.19 +1.2 55,946 + CCNA_00053 0.23 +1.5 55,959 - CCNA_00053 0.25 +3.0 55,964 - CCNA_00053 0.25 +1.8 55,979 + CCNA_00053 0.27 +0.4 55,979 + CCNA_00053 0.27 +0.7 55,979 + CCNA_00053 0.27 -1.6 55,987 - CCNA_00053 0.28 +1.2 55,987 - CCNA_00053 0.28 +1.8 55,987 - CCNA_00053 0.28 +1.4 55,987 - CCNA_00053 0.28 +1.6 55,987 - CCNA_00053 0.28 +0.9 55,987 - CCNA_00053 0.28 +2.1 55,987 - CCNA_00053 0.28 +1.4 56,007 - CCNA_00053 0.30 +1.5 56,025 - CCNA_00053 0.32 +0.9 56,025 - CCNA_00053 0.32 +1.6 56,049 + CCNA_00053 0.34 -0.7 56,049 + CCNA_00053 0.34 -0.1 56,050 + CCNA_00053 0.34 +0.6 56,089 - CCNA_00053 0.39 +1.6 56,089 - CCNA_00053 0.39 +1.6 56,186 + CCNA_00053 0.49 -0.7 56,209 + CCNA_00053 0.51 +0.7 56,250 - CCNA_00053 0.56 +1.4 56,270 + CCNA_00053 0.58 +0.7 56,288 - CCNA_00053 0.60 +2.0 56,299 - CCNA_00053 0.61 +0.8 56,319 - CCNA_00053 0.63 +1.5 56,327 + CCNA_00053 0.64 +0.1 56,334 - CCNA_00053 0.65 +1.8 56,357 + CCNA_00053 0.67 +1.7 56,364 + CCNA_00053 0.68 +0.3 56,372 - CCNA_00053 0.69 +1.4 56,390 - CCNA_00053 0.70 +1.3 56,405 + CCNA_00053 0.72 +0.7 56,418 - CCNA_00053 0.73 +1.7 56,432 + CCNA_00053 0.75 +1.0 56,450 - CCNA_00053 0.77 -0.3 56,474 + CCNA_00053 0.79 +1.0 56,484 - CCNA_00053 0.80 +2.1 56,491 - CCNA_00053 0.81 +2.0 56,502 + CCNA_00053 0.82 +0.5 56,533 + CCNA_00053 0.86 +1.3 56,566 + CCNA_00053 0.89 -0.7 56,566 + CCNA_00053 0.89 +0.8 56,574 - +1.4 56,574 - +1.5 56,577 + +1.3 56,577 + +0.0 56,582 + +0.6 56,618 + +0.8 56,618 + +0.4 56,639 - +1.1 56,648 + +0.0 56,651 - +0.8 56,656 - +1.2 56,656 - +1.5 56,658 - +1.8 56,674 + -1.2 56,682 - +0.3 56,684 + -0.8 56,687 - +0.4 56,692 - +0.3 56,692 - +0.6 56,707 + -0.1 56,715 - +2.6 56,734 + +0.6 56,780 + +1.5 56,795 - +2.0 56,819 - CCNA_00054 0.11 +2.0 56,860 + CCNA_00054 0.14 +2.3 56,912 - CCNA_00054 0.18 +2.6 56,912 - CCNA_00054 0.18 +2.4 56,927 + CCNA_00054 0.19 +2.3 56,927 + CCNA_00054 0.19 +1.7 56,935 - CCNA_00054 0.20 +2.9 56,935 - CCNA_00054 0.20 +2.3 56,935 - CCNA_00054 0.20 +2.0 57,034 + CCNA_00054 0.27 +2.5 57,041 - CCNA_00054 0.28 +2.6 57,041 - CCNA_00054 0.28 +1.0 57,069 - CCNA_00054 0.30 +3.1 57,080 + CCNA_00054 0.31 -0.7 57,138 + CCNA_00054 0.35 +2.8 57,138 + CCNA_00054 0.35 +2.3 57,146 - CCNA_00054 0.36 +2.6 57,148 + CCNA_00054 0.36 +1.3 57,148 + CCNA_00054 0.36 +1.3 57,148 + CCNA_00054 0.36 +1.8 57,156 - CCNA_00054 0.36 +3.3 57,156 - CCNA_00054 0.36 +2.8 57,156 - CCNA_00054 0.36 +3.3 57,156 - CCNA_00054 0.36 +2.6 57,156 - CCNA_00054 0.36 +3.3 57,156 - CCNA_00054 0.36 +3.0 57,169 + CCNA_00054 0.37 +1.3 57,169 + CCNA_00054 0.37 +3.0 57,177 - CCNA_00054 0.38 +3.0 57,177 - CCNA_00054 0.38 +2.6 57,177 - CCNA_00054 0.38 +2.3 57,194 + CCNA_00054 0.39 +3.3 57,256 + CCNA_00054 0.44 +1.7 57,307 + CCNA_00054 0.48 +1.3 57,315 - CCNA_00054 0.48 +2.9 57,320 + CCNA_00054 0.49 +0.7 57,352 + CCNA_00054 0.51 +2.7 57,352 + CCNA_00054 0.51 +1.8 57,375 - CCNA_00054 0.53 +3.0 57,387 + CCNA_00054 0.54 +3.3 57,395 - CCNA_00054 0.54 +2.0 57,412 + CCNA_00054 0.55 +1.4 57,419 - CCNA_00054 0.56 +2.6 57,419 - CCNA_00054 0.56 +3.2 57,428 + CCNA_00054 0.57 +2.9 57,428 + CCNA_00054 0.57 +1.7 57,487 + CCNA_00054 0.61 +1.3 57,492 - CCNA_00054 0.61 +2.4 57,495 - CCNA_00054 0.62 +2.7 57,511 - CCNA_00054 0.63 +3.2 57,511 - CCNA_00054 0.63 +2.6 57,531 - CCNA_00054 0.64 +2.4 57,588 - CCNA_00054 0.69 +3.0 57,623 - CCNA_00054 0.71 +2.8 57,665 + CCNA_00054 0.74 +2.3 57,733 - CCNA_00054 0.79 +3.2 57,738 - CCNA_00054 0.80 +3.2 57,768 - CCNA_00054 0.82 +2.8 57,816 + CCNA_00054 0.85 +3.3 57,829 - CCNA_00054 0.86 +2.5 57,829 - CCNA_00054 0.86 +0.9 57,852 + CCNA_00054 0.88 +2.0 57,868 - CCNA_00054 0.89 +2.7 57,868 - CCNA_00054 0.89 +2.4 57,870 - CCNA_00054 0.89 +3.1 57,892 - +3.2 57,910 + +1.9 57,913 - +2.9 57,932 + +1.0 57,940 - +3.3 57,946 + +2.3 57,954 - +3.2 58,001 + -0.2 58,027 - +3.0 58,035 + +2.6 58,043 + -0.1 58,043 + -1.5 58,043 - +3.0 58,051 - +0.8 58,058 - +0.2 58,092 + -0.1 58,111 + -0.3 58,111 + -0.7 58,116 + +0.0 58,116 + -1.4 58,124 - +0.5 58,124 - +0.6 58,178 - +0.4 58,179 - +0.7 58,192 + -1.9 58,192 - +1.2 58,192 - +1.1 58,623 - -2.5 58,651 - -3.3 58,675 + +0.7 58,709 + CCNA_00056 0.12 +1.9 58,733 + CCNA_00056 0.17 +1.8 58,742 - CCNA_00056 0.19 +1.2 58,751 + CCNA_00056 0.21 +0.5 58,751 + CCNA_00056 0.21 +0.4 58,759 - CCNA_00056 0.23 -0.3 58,759 - +0.7 58,759 - CCNA_00056 0.23 +0.5 58,764 - CCNA_00056 0.24 +0.9 58,811 + CCNA_00056 0.35 +1.6 58,819 - CCNA_00056 0.36 +1.6 58,819 - CCNA_00056 0.36 +1.4 58,819 - CCNA_00056 0.36 +0.8 58,841 + CCNA_00056 0.41 +1.3 58,849 - CCNA_00056 0.43 +2.8 58,854 - CCNA_00056 0.44 +1.8 58,854 - CCNA_00056 0.44 +2.0 58,854 - CCNA_00056 0.44 +1.6 58,943 - CCNA_00056 0.64 +1.8 58,943 - CCNA_00056 0.64 +1.4 58,943 - CCNA_00056 0.64 +1.6 58,943 - CCNA_00056 0.64 +2.0 58,951 - CCNA_00056 0.66 +0.7
Or see this region's nucleotide sequence