Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS25520

Experiment: L-Threonine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS25515 and BWI76_RS25520 overlap by 1 nucleotidesBWI76_RS25520 and BWI76_RS25525 are separated by 121 nucleotidesBWI76_RS25525 and BWI76_RS25530 are separated by 9 nucleotidesBWI76_RS25530 and BWI76_RS25535 overlap by 28 nucleotidesBWI76_RS25535 and BWI76_RS25540 are separated by 10 nucleotides BWI76_RS25515: BWI76_RS25515 - sulfoxide reductase, at 5,305,065 to 5,306,066 _RS25515 BWI76_RS25520: BWI76_RS25520 - sulfoxide reductase heme-binding subunit YedZ, at 5,306,066 to 5,306,665 _RS25520 BWI76_RS25525: BWI76_RS25525 - hypothetical protein, at 5,306,787 to 5,306,987 _RS25525 BWI76_RS25530: BWI76_RS25530 - acetyl-CoA carboxylase, at 5,306,997 to 5,307,086 _RS25530 BWI76_RS25535: BWI76_RS25535 - acetyl-CoA carboxylase biotin carboxyl carrier protein subunit, at 5,307,059 to 5,307,532 _RS25535 BWI76_RS25540: BWI76_RS25540 - acetyl-CoA carboxylase biotin carboxylase subunit, at 5,307,543 to 5,308,892 _RS25540 Position (kb) 5306 5307Strain fitness (log2 ratio) -1 0 1 2at 5305.165 kb on + strandat 5305.165 kb on + strandat 5305.173 kb on - strand, within BWI76_RS25515at 5305.228 kb on + strand, within BWI76_RS25515at 5305.264 kb on - strand, within BWI76_RS25515at 5305.346 kb on + strand, within BWI76_RS25515at 5305.354 kb on - strand, within BWI76_RS25515at 5305.354 kb on - strand, within BWI76_RS25515at 5305.439 kb on + strand, within BWI76_RS25515at 5305.620 kb on - strand, within BWI76_RS25515at 5305.716 kb on - strand, within BWI76_RS25515at 5305.726 kb on - strandat 5305.760 kb on - strand, within BWI76_RS25515at 5305.837 kb on + strand, within BWI76_RS25515at 5306.162 kb on - strand, within BWI76_RS25520at 5306.346 kb on + strand, within BWI76_RS25520at 5306.346 kb on + strand, within BWI76_RS25520at 5306.346 kb on + strand, within BWI76_RS25520at 5306.346 kb on + strand, within BWI76_RS25520at 5306.346 kb on + strand, within BWI76_RS25520at 5306.354 kb on - strand, within BWI76_RS25520at 5306.354 kb on - strand, within BWI76_RS25520at 5306.354 kb on - strand, within BWI76_RS25520at 5306.354 kb on - strand, within BWI76_RS25520at 5306.354 kb on - strand, within BWI76_RS25520at 5306.386 kb on + strand, within BWI76_RS25520at 5306.503 kb on + strand, within BWI76_RS25520at 5306.575 kb on + strand, within BWI76_RS25520

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Threonine (N)
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5,305,165 + +1.4
5,305,165 + -0.3
5,305,173 - BWI76_RS25515 0.11 +0.3
5,305,228 + BWI76_RS25515 0.16 +0.4
5,305,264 - BWI76_RS25515 0.20 +0.3
5,305,346 + BWI76_RS25515 0.28 +1.6
5,305,354 - BWI76_RS25515 0.29 +0.3
5,305,354 - BWI76_RS25515 0.29 +0.6
5,305,439 + BWI76_RS25515 0.37 +0.8
5,305,620 - BWI76_RS25515 0.55 -0.2
5,305,716 - BWI76_RS25515 0.65 -0.1
5,305,726 - -0.5
5,305,760 - BWI76_RS25515 0.69 +0.2
5,305,837 + BWI76_RS25515 0.77 +0.5
5,306,162 - BWI76_RS25520 0.16 -0.5
5,306,346 + BWI76_RS25520 0.47 -0.2
5,306,346 + BWI76_RS25520 0.47 +0.5
5,306,346 + BWI76_RS25520 0.47 +1.3
5,306,346 + BWI76_RS25520 0.47 -0.5
5,306,346 + BWI76_RS25520 0.47 -1.0
5,306,354 - BWI76_RS25520 0.48 -0.0
5,306,354 - BWI76_RS25520 0.48 -0.1
5,306,354 - BWI76_RS25520 0.48 -0.0
5,306,354 - BWI76_RS25520 0.48 -0.0
5,306,354 - BWI76_RS25520 0.48 +1.9
5,306,386 + BWI76_RS25520 0.53 +0.1
5,306,503 + BWI76_RS25520 0.73 +0.2
5,306,575 + BWI76_RS25520 0.85 +0.7

Or see this region's nucleotide sequence