Experiment: L-Threonine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24205 and BWI76_RS24210 are separated by 10 nucleotides BWI76_RS24210 and BWI76_RS24215 are separated by 79 nucleotides
BWI76_RS24205: BWI76_RS24205 - cystathionine beta-synthase, at 5,045,495 to 5,046,865
_RS24205
BWI76_RS24210: BWI76_RS24210 - cystathionine gamma-lyase, at 5,046,876 to 5,048,021
_RS24210
BWI76_RS24215: BWI76_RS24215 - LysR family transcriptional regulator, at 5,048,101 to 5,049,000
_RS24215
Position (kb)
5046
5047
5048
5049 Strain fitness (log2 ratio)
-1
0
1
2 at 5046.084 kb on - strand, within BWI76_RS24205 at 5046.289 kb on + strand, within BWI76_RS24205 at 5046.307 kb on + strand, within BWI76_RS24205 at 5046.328 kb on + strand, within BWI76_RS24205 at 5046.655 kb on + strand, within BWI76_RS24205 at 5046.720 kb on - strand, within BWI76_RS24205 at 5046.965 kb on - strand at 5047.117 kb on + strand, within BWI76_RS24210 at 5047.185 kb on - strand, within BWI76_RS24210 at 5047.195 kb on - strand, within BWI76_RS24210 at 5047.503 kb on - strand, within BWI76_RS24210 at 5047.596 kb on - strand, within BWI76_RS24210 at 5047.695 kb on + strand, within BWI76_RS24210 at 5047.816 kb on + strand, within BWI76_RS24210 at 5047.850 kb on + strand, within BWI76_RS24210 at 5048.010 kb on - strand at 5048.098 kb on + strand at 5048.098 kb on + strand at 5048.219 kb on + strand, within BWI76_RS24215 at 5048.249 kb on - strand, within BWI76_RS24215 at 5048.352 kb on - strand, within BWI76_RS24215 at 5048.645 kb on + strand, within BWI76_RS24215 at 5048.751 kb on + strand, within BWI76_RS24215 at 5048.872 kb on - strand, within BWI76_RS24215 at 5049.017 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Threonine (N) remove 5,046,084 - BWI76_RS24205 0.43 -0.0 5,046,289 + BWI76_RS24205 0.58 +0.5 5,046,307 + BWI76_RS24205 0.59 +1.0 5,046,328 + BWI76_RS24205 0.61 +1.2 5,046,655 + BWI76_RS24205 0.85 -0.7 5,046,720 - BWI76_RS24205 0.89 -0.9 5,046,965 - +0.8 5,047,117 + BWI76_RS24210 0.21 +0.5 5,047,185 - BWI76_RS24210 0.27 -1.6 5,047,195 - BWI76_RS24210 0.28 -0.3 5,047,503 - BWI76_RS24210 0.55 +0.2 5,047,596 - BWI76_RS24210 0.63 +0.1 5,047,695 + BWI76_RS24210 0.71 +0.2 5,047,816 + BWI76_RS24210 0.82 +0.2 5,047,850 + BWI76_RS24210 0.85 +0.7 5,048,010 - +0.4 5,048,098 + -0.8 5,048,098 + +0.0 5,048,219 + BWI76_RS24215 0.13 +0.3 5,048,249 - BWI76_RS24215 0.16 -0.9 5,048,352 - BWI76_RS24215 0.28 -1.4 5,048,645 + BWI76_RS24215 0.60 +2.3 5,048,751 + BWI76_RS24215 0.72 +1.4 5,048,872 - BWI76_RS24215 0.86 -1.1 5,049,017 - +0.9
Or see this region's nucleotide sequence