Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS24030

Experiment: L-Threonine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS24025 and BWI76_RS24030 are separated by 211 nucleotidesBWI76_RS24030 and BWI76_RS24035 are separated by 8 nucleotidesBWI76_RS24035 and BWI76_RS24040 are separated by 118 nucleotidesBWI76_RS24040 and BWI76_RS24045 are separated by 15 nucleotidesBWI76_RS24045 and BWI76_RS24050 are separated by 62 nucleotides BWI76_RS24025: BWI76_RS24025 - agmatinase, at 5,014,228 to 5,015,148 _RS24025 BWI76_RS24030: BWI76_RS24030 - arginine decarboxylase, at 5,015,360 to 5,017,336 _RS24030 BWI76_RS24035: BWI76_RS24035 - hypothetical protein, at 5,017,345 to 5,017,491 _RS24035 BWI76_RS24040: BWI76_RS24040 - hypothetical protein, at 5,017,610 to 5,017,855 _RS24040 BWI76_RS24045: BWI76_RS24045 - hypothetical protein, at 5,017,871 to 5,018,116 _RS24045 BWI76_RS24050: BWI76_RS24050 - methionine adenosyltransferase, at 5,018,179 to 5,019,333 _RS24050 Position (kb) 5015 5016 5017 5018Strain fitness (log2 ratio) -3 -2 -1 0 1at 5014.443 kb on + strand, within BWI76_RS24025at 5014.495 kb on + strand, within BWI76_RS24025at 5014.605 kb on - strand, within BWI76_RS24025at 5014.643 kb on + strand, within BWI76_RS24025at 5014.643 kb on + strand, within BWI76_RS24025at 5014.643 kb on + strand, within BWI76_RS24025at 5014.651 kb on - strand, within BWI76_RS24025at 5014.891 kb on - strand, within BWI76_RS24025at 5015.150 kb on + strandat 5015.644 kb on + strand, within BWI76_RS24030at 5016.002 kb on - strand, within BWI76_RS24030at 5016.088 kb on - strand, within BWI76_RS24030at 5016.255 kb on + strand, within BWI76_RS24030at 5016.289 kb on + strand, within BWI76_RS24030at 5016.451 kb on + strand, within BWI76_RS24030at 5016.543 kb on - strand, within BWI76_RS24030at 5016.881 kb on - strand, within BWI76_RS24030at 5016.923 kb on - strand, within BWI76_RS24030at 5016.949 kb on + strand, within BWI76_RS24030at 5017.062 kb on + strand, within BWI76_RS24030at 5017.096 kb on - strand, within BWI76_RS24030at 5017.314 kb on + strandat 5017.322 kb on - strandat 5017.408 kb on + strand, within BWI76_RS24035at 5017.408 kb on + strand, within BWI76_RS24035at 5017.416 kb on - strand, within BWI76_RS24035at 5017.433 kb on + strand, within BWI76_RS24035at 5017.446 kb on - strand, within BWI76_RS24035at 5017.497 kb on - strandat 5017.503 kb on + strandat 5017.503 kb on + strandat 5017.511 kb on - strandat 5017.511 kb on - strandat 5017.511 kb on - strandat 5017.511 kb on - strandat 5017.563 kb on - strandat 5017.592 kb on + strandat 5017.602 kb on - strandat 5017.620 kb on - strandat 5017.620 kb on - strandat 5017.629 kb on + strandat 5017.651 kb on - strand, within BWI76_RS24040at 5017.724 kb on + strand, within BWI76_RS24040at 5017.756 kb on + strand, within BWI76_RS24040at 5017.829 kb on - strand, within BWI76_RS24040at 5017.868 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Threonine (N)
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5,014,443 + BWI76_RS24025 0.23 -1.4
5,014,495 + BWI76_RS24025 0.29 -1.3
5,014,605 - BWI76_RS24025 0.41 -2.3
5,014,643 + BWI76_RS24025 0.45 -1.9
5,014,643 + BWI76_RS24025 0.45 -2.3
5,014,643 + BWI76_RS24025 0.45 -1.8
5,014,651 - BWI76_RS24025 0.46 -3.5
5,014,891 - BWI76_RS24025 0.72 -2.3
5,015,150 + -0.1
5,015,644 + BWI76_RS24030 0.14 +0.3
5,016,002 - BWI76_RS24030 0.32 -0.5
5,016,088 - BWI76_RS24030 0.37 -1.1
5,016,255 + BWI76_RS24030 0.45 -0.4
5,016,289 + BWI76_RS24030 0.47 -0.2
5,016,451 + BWI76_RS24030 0.55 +0.5
5,016,543 - BWI76_RS24030 0.60 -0.0
5,016,881 - BWI76_RS24030 0.77 +0.0
5,016,923 - BWI76_RS24030 0.79 +0.6
5,016,949 + BWI76_RS24030 0.80 -0.4
5,017,062 + BWI76_RS24030 0.86 -1.2
5,017,096 - BWI76_RS24030 0.88 -1.3
5,017,314 + -0.3
5,017,322 - -0.9
5,017,408 + BWI76_RS24035 0.43 +0.8
5,017,408 + BWI76_RS24035 0.43 -0.0
5,017,416 - BWI76_RS24035 0.48 +1.3
5,017,433 + BWI76_RS24035 0.60 +0.2
5,017,446 - BWI76_RS24035 0.69 -0.1
5,017,497 - -0.5
5,017,503 + +0.2
5,017,503 + +0.8
5,017,511 - +0.2
5,017,511 - -0.2
5,017,511 - +0.1
5,017,511 - +0.2
5,017,563 - +0.5
5,017,592 + -0.0
5,017,602 - +0.3
5,017,620 - +0.9
5,017,620 - +0.3
5,017,629 + -0.1
5,017,651 - BWI76_RS24040 0.17 +0.4
5,017,724 + BWI76_RS24040 0.46 -0.3
5,017,756 + BWI76_RS24040 0.59 +0.8
5,017,829 - BWI76_RS24040 0.89 +0.4
5,017,868 + -0.0

Or see this region's nucleotide sequence