Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20780

Experiment: L-Threonine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20775 and BWI76_RS20780 are separated by 170 nucleotidesBWI76_RS20780 and BWI76_RS20785 overlap by 11 nucleotidesBWI76_RS20785 and BWI76_RS20790 are separated by 10 nucleotides BWI76_RS20775: BWI76_RS20775 - ethanolamine utilization protein EutH, at 4,342,470 to 4,343,702 _RS20775 BWI76_RS20780: BWI76_RS20780 - alcohol dehydrogenase EutG, at 4,343,873 to 4,345,060 _RS20780 BWI76_RS20785: BWI76_RS20785 - ethanolamine utilization protein EutJ, at 4,345,050 to 4,345,889 _RS20785 BWI76_RS20790: BWI76_RS20790 - aldehyde dehydrogenase, at 4,345,900 to 4,347,303 _RS20790 Position (kb) 4343 4344 4345 4346Strain fitness (log2 ratio) -2 -1 0 1 2at 4343.138 kb on - strand, within BWI76_RS20775at 4343.138 kb on - strand, within BWI76_RS20775at 4343.335 kb on + strand, within BWI76_RS20775at 4343.343 kb on - strand, within BWI76_RS20775at 4343.551 kb on - strand, within BWI76_RS20775at 4343.655 kb on - strandat 4343.675 kb on - strandat 4343.764 kb on + strandat 4343.826 kb on + strandat 4343.859 kb on + strandat 4344.162 kb on + strand, within BWI76_RS20780at 4344.162 kb on + strand, within BWI76_RS20780at 4344.170 kb on - strand, within BWI76_RS20780at 4344.227 kb on + strand, within BWI76_RS20780at 4344.306 kb on + strand, within BWI76_RS20780at 4344.398 kb on + strand, within BWI76_RS20780at 4344.467 kb on + strand, within BWI76_RS20780at 4344.669 kb on - strand, within BWI76_RS20780at 4344.669 kb on - strand, within BWI76_RS20780at 4344.876 kb on - strand, within BWI76_RS20780at 4344.993 kb on + strandat 4345.529 kb on + strand, within BWI76_RS20785at 4345.579 kb on - strandat 4345.916 kb on + strandat 4346.010 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Threonine (N)
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4,343,138 - BWI76_RS20775 0.54 +0.1
4,343,138 - BWI76_RS20775 0.54 +0.3
4,343,335 + BWI76_RS20775 0.70 +1.0
4,343,343 - BWI76_RS20775 0.71 +1.1
4,343,551 - BWI76_RS20775 0.88 -1.2
4,343,655 - +0.2
4,343,675 - -0.6
4,343,764 + -0.6
4,343,826 + -0.0
4,343,859 + -0.4
4,344,162 + BWI76_RS20780 0.24 +0.5
4,344,162 + BWI76_RS20780 0.24 -0.9
4,344,170 - BWI76_RS20780 0.25 -0.4
4,344,227 + BWI76_RS20780 0.30 -2.6
4,344,306 + BWI76_RS20780 0.36 +0.4
4,344,398 + BWI76_RS20780 0.44 -0.1
4,344,467 + BWI76_RS20780 0.50 -0.8
4,344,669 - BWI76_RS20780 0.67 +0.1
4,344,669 - BWI76_RS20780 0.67 +0.7
4,344,876 - BWI76_RS20780 0.84 -0.0
4,344,993 + +1.1
4,345,529 + BWI76_RS20785 0.57 +0.5
4,345,579 - +2.2
4,345,916 + +0.4
4,346,010 - +0.7

Or see this region's nucleotide sequence