Experiment: L-Threonine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05970 and BWI76_RS05975 are separated by 107 nucleotides BWI76_RS05975 and BWI76_RS05980 overlap by 8 nucleotides BWI76_RS05980 and BWI76_RS05985 overlap by 14 nucleotides
BWI76_RS05970: BWI76_RS05970 - hypothetical protein, at 1,253,745 to 1,253,927
_RS05970
BWI76_RS05975: BWI76_RS05975 - ABC transporter ATP-binding protein, at 1,254,035 to 1,254,730
_RS05975
BWI76_RS05980: BWI76_RS05980 - ABC transporter ATP-binding protein, at 1,254,723 to 1,255,505
_RS05980
BWI76_RS05985: BWI76_RS05985 - branched-chain amino acid ABC transporter permease, at 1,255,492 to 1,256,541
_RS05985
Position (kb)
1254
1255
1256 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1253.798 kb on + strand, within BWI76_RS05970 at 1253.880 kb on - strand, within BWI76_RS05970 at 1254.022 kb on + strand at 1254.030 kb on - strand at 1254.030 kb on - strand at 1254.030 kb on - strand at 1254.062 kb on - strand at 1254.255 kb on - strand, within BWI76_RS05975 at 1254.403 kb on + strand, within BWI76_RS05975 at 1254.411 kb on - strand, within BWI76_RS05975 at 1254.432 kb on + strand, within BWI76_RS05975 at 1254.675 kb on + strand at 1254.794 kb on - strand at 1254.816 kb on - strand, within BWI76_RS05980 at 1254.856 kb on + strand, within BWI76_RS05980 at 1255.055 kb on - strand, within BWI76_RS05980 at 1255.099 kb on + strand, within BWI76_RS05980 at 1255.099 kb on + strand, within BWI76_RS05980 at 1255.182 kb on + strand, within BWI76_RS05980 at 1255.338 kb on - strand, within BWI76_RS05980 at 1255.352 kb on + strand at 1255.384 kb on + strand, within BWI76_RS05980 at 1255.465 kb on + strand at 1255.831 kb on + strand, within BWI76_RS05985 at 1256.049 kb on - strand, within BWI76_RS05985 at 1256.193 kb on + strand, within BWI76_RS05985 at 1256.366 kb on - strand, within BWI76_RS05985 at 1256.382 kb on - strand, within BWI76_RS05985 at 1256.441 kb on - strand at 1256.501 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Threonine (N) remove 1,253,798 + BWI76_RS05970 0.29 -0.1 1,253,880 - BWI76_RS05970 0.74 -0.2 1,254,022 + +1.4 1,254,030 - +0.5 1,254,030 - -0.9 1,254,030 - -2.2 1,254,062 - +0.4 1,254,255 - BWI76_RS05975 0.32 -0.4 1,254,403 + BWI76_RS05975 0.53 -0.6 1,254,411 - BWI76_RS05975 0.54 -3.6 1,254,432 + BWI76_RS05975 0.57 -1.3 1,254,675 + -3.9 1,254,794 - -4.1 1,254,816 - BWI76_RS05980 0.12 -3.0 1,254,856 + BWI76_RS05980 0.17 +1.8 1,255,055 - BWI76_RS05980 0.42 -3.0 1,255,099 + BWI76_RS05980 0.48 -2.0 1,255,099 + BWI76_RS05980 0.48 -2.5 1,255,182 + BWI76_RS05980 0.59 -2.6 1,255,338 - BWI76_RS05980 0.79 -4.3 1,255,352 + +0.8 1,255,384 + BWI76_RS05980 0.84 -1.9 1,255,465 + -2.8 1,255,831 + BWI76_RS05985 0.32 -2.0 1,256,049 - BWI76_RS05985 0.53 -4.5 1,256,193 + BWI76_RS05985 0.67 -1.2 1,256,366 - BWI76_RS05985 0.83 -1.0 1,256,382 - BWI76_RS05985 0.85 +0.0 1,256,441 - -3.1 1,256,501 - -0.4
Or see this region's nucleotide sequence