Experiment: Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt AZOBR_RS16700 and AZOBR_RS16705 are separated by 107 nucleotides AZOBR_RS16705 and AZOBR_RS16710 are separated by 2 nucleotides AZOBR_RS16710 and AZOBR_RS16715 are separated by 2 nucleotides
AZOBR_RS16700: AZOBR_RS16700 - precorrin-3B C17-methyltransferase, at 505,354 to 506,133
_RS16700
AZOBR_RS16705: AZOBR_RS16705 - cobalt-precorrin-6x reductase, at 506,241 to 506,975
_RS16705
AZOBR_RS16710: AZOBR_RS16710 - precorrin-6Y C5 15-methyltransferase, at 506,978 to 508,225
_RS16710
AZOBR_RS16715: AZOBR_RS16715 - cobalamin biosynthesis protein, at 508,228 to 508,632
_RS16715
Position (kb) 506
507
508
509 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 506.101 kb on + strand at 506.101 kb on + strand at 506.102 kb on - strand at 506.117 kb on - strand at 506.243 kb on - strand at 506.477 kb on - strand, within AZOBR_RS16705 at 506.477 kb on - strand, within AZOBR_RS16705 at 506.528 kb on - strand, within AZOBR_RS16705 at 507.005 kb on - strand at 507.005 kb on - strand at 507.260 kb on + strand, within AZOBR_RS16710 at 507.261 kb on - strand, within AZOBR_RS16710 at 508.888 kb on + strand at 509.170 kb on + strand at 509.170 kb on + strand at 509.170 kb on + strand at 509.171 kb on - strand at 509.171 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.2X; growth supplemented with 0.4X LB remove 506,101 + -0.1 506,101 + -2.4 506,102 - -0.2 506,117 - +0.3 506,243 - +0.3 506,477 - AZOBR_RS16705 0.32 -0.4 506,477 - AZOBR_RS16705 0.32 -2.8 506,528 - AZOBR_RS16705 0.39 +1.7 507,005 - -1.3 507,005 - -2.8 507,260 + AZOBR_RS16710 0.23 +0.5 507,261 - AZOBR_RS16710 0.23 +0.8 508,888 + -0.6 509,170 + +0.9 509,170 + +1.4 509,170 + +2.3 509,171 - -1.7 509,171 - +1.6
Or see this region's nucleotide sequence