Experiment: m.b. Fusidic 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c09040 and PGA1_c09050 overlap by 25 nucleotides PGA1_c09050 and PGA1_c09070 are separated by 23 nucleotides PGA1_c09070 and PGA1_c09090 are separated by 3 nucleotides PGA1_c09090 and PGA1_c09100 overlap by 32 nucleotides
PGA1_c09040: PGA1_c09040 - putative adenylyltransferase ThiF, at 931,995 to 932,984
_c09040
PGA1_c09050: PGA1_c09050 - thiamine-phosphate pyrophosphorylase ThiE, at 932,960 to 933,553
_c09050
PGA1_c09070: PGA1_c09070 - thiazole biosynthesis protein ThiG, at 933,577 to 934,344
_c09070
PGA1_c09090: PGA1_c09090 - thiamine biosynthesis protein ThiS, at 934,348 to 934,545
_c09090
PGA1_c09100: PGA1_c09100 - putative thiamine biosynthesis oxidoreductase ThiO, at 934,514 to 935,488
_c09100
Position (kb) 933
934
935 Strain fitness (log2 ratio) -1
0
1
2
3 at 932.578 kb on + strand, within PGA1_c09040 at 932.578 kb on + strand, within PGA1_c09040 at 932.578 kb on + strand, within PGA1_c09040 at 932.578 kb on + strand, within PGA1_c09040 at 932.580 kb on + strand, within PGA1_c09040 at 932.584 kb on - strand, within PGA1_c09040 at 932.586 kb on - strand, within PGA1_c09040 at 932.586 kb on - strand, within PGA1_c09040 at 932.586 kb on - strand, within PGA1_c09040 at 932.588 kb on + strand, within PGA1_c09040 at 932.588 kb on + strand, within PGA1_c09040 at 932.596 kb on - strand, within PGA1_c09040 at 932.708 kb on + strand, within PGA1_c09040 at 932.722 kb on + strand, within PGA1_c09040 at 932.757 kb on + strand, within PGA1_c09040 at 932.760 kb on - strand, within PGA1_c09040 at 932.786 kb on - strand, within PGA1_c09040 at 932.789 kb on + strand, within PGA1_c09040 at 932.800 kb on + strand, within PGA1_c09040 at 932.849 kb on + strand, within PGA1_c09040 at 932.859 kb on + strand, within PGA1_c09040 at 932.859 kb on + strand, within PGA1_c09040 at 932.862 kb on - strand, within PGA1_c09040 at 932.981 kb on - strand at 932.981 kb on - strand at 932.981 kb on - strand at 933.114 kb on + strand, within PGA1_c09050 at 933.146 kb on + strand, within PGA1_c09050 at 933.154 kb on - strand, within PGA1_c09050 at 933.184 kb on - strand, within PGA1_c09050 at 933.186 kb on + strand, within PGA1_c09050 at 933.186 kb on + strand, within PGA1_c09050 at 933.213 kb on - strand, within PGA1_c09050 at 933.275 kb on + strand, within PGA1_c09050 at 933.283 kb on - strand, within PGA1_c09050 at 933.283 kb on - strand, within PGA1_c09050 at 933.312 kb on - strand, within PGA1_c09050 at 933.314 kb on + strand, within PGA1_c09050 at 933.314 kb on + strand, within PGA1_c09050 at 933.322 kb on - strand, within PGA1_c09050 at 933.322 kb on - strand, within PGA1_c09050 at 933.484 kb on + strand, within PGA1_c09050 at 933.488 kb on + strand, within PGA1_c09050 at 933.518 kb on + strand at 933.575 kb on + strand at 933.580 kb on + strand at 933.581 kb on - strand at 933.583 kb on - strand at 933.583 kb on - strand at 933.583 kb on - strand at 933.583 kb on - strand at 933.603 kb on - strand at 933.689 kb on + strand, within PGA1_c09070 at 933.702 kb on - strand, within PGA1_c09070 at 933.731 kb on + strand, within PGA1_c09070 at 933.731 kb on + strand, within PGA1_c09070 at 933.739 kb on - strand, within PGA1_c09070 at 933.767 kb on + strand, within PGA1_c09070 at 933.862 kb on + strand, within PGA1_c09070 at 933.865 kb on - strand, within PGA1_c09070 at 933.878 kb on + strand, within PGA1_c09070 at 933.881 kb on - strand, within PGA1_c09070 at 933.901 kb on - strand, within PGA1_c09070 at 933.948 kb on + strand, within PGA1_c09070 at 933.972 kb on + strand, within PGA1_c09070 at 933.978 kb on - strand, within PGA1_c09070 at 933.980 kb on - strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.004 kb on + strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.012 kb on - strand, within PGA1_c09070 at 934.076 kb on + strand, within PGA1_c09070 at 934.084 kb on - strand, within PGA1_c09070 at 934.103 kb on - strand, within PGA1_c09070 at 934.103 kb on - strand, within PGA1_c09070 at 934.108 kb on + strand, within PGA1_c09070 at 934.142 kb on + strand, within PGA1_c09070 at 934.142 kb on + strand, within PGA1_c09070 at 934.150 kb on - strand, within PGA1_c09070 at 934.152 kb on + strand, within PGA1_c09070 at 934.152 kb on + strand, within PGA1_c09070 at 934.162 kb on + strand, within PGA1_c09070 at 934.275 kb on + strand at 934.275 kb on - strand at 934.306 kb on - strand at 934.311 kb on + strand at 934.311 kb on + strand at 934.311 kb on + strand at 934.319 kb on - strand at 934.339 kb on - strand at 934.354 kb on - strand at 934.364 kb on + strand at 934.472 kb on - strand, within PGA1_c09090 at 934.513 kb on - strand, within PGA1_c09090 at 934.529 kb on - strand at 934.539 kb on + strand at 934.568 kb on - strand at 934.624 kb on - strand, within PGA1_c09100 at 934.636 kb on - strand, within PGA1_c09100 at 934.659 kb on - strand, within PGA1_c09100 at 934.659 kb on - strand, within PGA1_c09100 at 934.685 kb on + strand, within PGA1_c09100 at 934.685 kb on + strand, within PGA1_c09100 at 934.693 kb on - strand, within PGA1_c09100 at 934.693 kb on - strand, within PGA1_c09100 at 934.693 kb on - strand, within PGA1_c09100 at 934.710 kb on + strand, within PGA1_c09100 at 934.719 kb on - strand, within PGA1_c09100 at 934.846 kb on + strand, within PGA1_c09100 at 934.854 kb on - strand, within PGA1_c09100 at 934.854 kb on - strand, within PGA1_c09100 at 934.903 kb on + strand, within PGA1_c09100 at 934.943 kb on + strand, within PGA1_c09100 at 935.068 kb on - strand, within PGA1_c09100 at 935.081 kb on + strand, within PGA1_c09100 at 935.089 kb on - strand, within PGA1_c09100 at 935.139 kb on + strand, within PGA1_c09100 at 935.147 kb on - strand, within PGA1_c09100 at 935.147 kb on - strand, within PGA1_c09100 at 935.172 kb on + strand, within PGA1_c09100 at 935.177 kb on + strand, within PGA1_c09100 at 935.217 kb on - strand, within PGA1_c09100 at 935.228 kb on - strand, within PGA1_c09100 at 935.276 kb on - strand, within PGA1_c09100
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Fusidic 0.0002 mg/ml remove 932,578 + PGA1_c09040 0.59 +0.8 932,578 + PGA1_c09040 0.59 +0.2 932,578 + PGA1_c09040 0.59 +0.4 932,578 + PGA1_c09040 0.59 +1.2 932,580 + PGA1_c09040 0.59 +2.0 932,584 - PGA1_c09040 0.59 +1.9 932,586 - PGA1_c09040 0.60 +1.4 932,586 - PGA1_c09040 0.60 +1.2 932,586 - PGA1_c09040 0.60 +0.3 932,588 + PGA1_c09040 0.60 +1.2 932,588 + PGA1_c09040 0.60 +1.3 932,596 - PGA1_c09040 0.61 +2.9 932,708 + PGA1_c09040 0.72 +3.1 932,722 + PGA1_c09040 0.73 +0.8 932,757 + PGA1_c09040 0.77 +1.9 932,760 - PGA1_c09040 0.77 +0.5 932,786 - PGA1_c09040 0.80 +0.6 932,789 + PGA1_c09040 0.80 +1.8 932,800 + PGA1_c09040 0.81 +1.5 932,849 + PGA1_c09040 0.86 +1.9 932,859 + PGA1_c09040 0.87 +1.7 932,859 + PGA1_c09040 0.87 +2.4 932,862 - PGA1_c09040 0.88 +1.6 932,981 - -0.8 932,981 - +1.1 932,981 - -0.4 933,114 + PGA1_c09050 0.26 +1.5 933,146 + PGA1_c09050 0.31 +1.3 933,154 - PGA1_c09050 0.33 +2.0 933,184 - PGA1_c09050 0.38 +2.6 933,186 + PGA1_c09050 0.38 +2.0 933,186 + PGA1_c09050 0.38 +1.1 933,213 - PGA1_c09050 0.43 +1.0 933,275 + PGA1_c09050 0.53 +1.2 933,283 - PGA1_c09050 0.54 +1.0 933,283 - PGA1_c09050 0.54 +1.4 933,312 - PGA1_c09050 0.59 +1.9 933,314 + PGA1_c09050 0.60 +1.6 933,314 + PGA1_c09050 0.60 +1.0 933,322 - PGA1_c09050 0.61 +2.2 933,322 - PGA1_c09050 0.61 +1.4 933,484 + PGA1_c09050 0.88 +1.7 933,488 + PGA1_c09050 0.89 +2.0 933,518 + +2.1 933,575 + +0.6 933,580 + +1.2 933,581 - +0.4 933,583 - +0.3 933,583 - +0.1 933,583 - -0.1 933,583 - -0.7 933,603 - +1.5 933,689 + PGA1_c09070 0.15 +2.3 933,702 - PGA1_c09070 0.16 +2.0 933,731 + PGA1_c09070 0.20 +2.1 933,731 + PGA1_c09070 0.20 +1.7 933,739 - PGA1_c09070 0.21 +2.6 933,767 + PGA1_c09070 0.25 +1.5 933,862 + PGA1_c09070 0.37 +0.9 933,865 - PGA1_c09070 0.38 +1.2 933,878 + PGA1_c09070 0.39 +1.8 933,881 - PGA1_c09070 0.40 +1.8 933,901 - PGA1_c09070 0.42 +1.4 933,948 + PGA1_c09070 0.48 +2.2 933,972 + PGA1_c09070 0.51 +1.5 933,978 - PGA1_c09070 0.52 +2.3 933,980 - PGA1_c09070 0.52 +0.9 934,004 + PGA1_c09070 0.56 +1.0 934,004 + PGA1_c09070 0.56 +1.2 934,004 + PGA1_c09070 0.56 +1.6 934,004 + PGA1_c09070 0.56 +2.3 934,004 + PGA1_c09070 0.56 +1.4 934,004 + PGA1_c09070 0.56 +1.3 934,004 + PGA1_c09070 0.56 +2.0 934,004 + PGA1_c09070 0.56 +1.1 934,012 - PGA1_c09070 0.57 +2.6 934,012 - PGA1_c09070 0.57 +0.6 934,012 - PGA1_c09070 0.57 +1.7 934,012 - PGA1_c09070 0.57 +1.7 934,012 - PGA1_c09070 0.57 +1.0 934,012 - PGA1_c09070 0.57 +2.1 934,012 - PGA1_c09070 0.57 +0.8 934,012 - PGA1_c09070 0.57 +1.6 934,012 - PGA1_c09070 0.57 +0.9 934,076 + PGA1_c09070 0.65 +1.0 934,084 - PGA1_c09070 0.66 +1.0 934,103 - PGA1_c09070 0.68 +1.2 934,103 - PGA1_c09070 0.68 +2.1 934,108 + PGA1_c09070 0.69 +2.3 934,142 + PGA1_c09070 0.74 +2.1 934,142 + PGA1_c09070 0.74 +1.1 934,150 - PGA1_c09070 0.75 +2.2 934,152 + PGA1_c09070 0.75 +0.3 934,152 + PGA1_c09070 0.75 +1.6 934,162 + PGA1_c09070 0.76 +1.9 934,275 + +1.4 934,275 - +1.7 934,306 - +1.9 934,311 + -0.2 934,311 + +1.2 934,311 + +2.2 934,319 - +1.7 934,339 - +2.3 934,354 - +1.6 934,364 + +1.4 934,472 - PGA1_c09090 0.63 +2.3 934,513 - PGA1_c09090 0.83 +1.1 934,529 - +1.7 934,539 + +2.2 934,568 - +1.2 934,624 - PGA1_c09100 0.11 +1.5 934,636 - PGA1_c09100 0.13 +0.4 934,659 - PGA1_c09100 0.15 +1.2 934,659 - PGA1_c09100 0.15 +1.7 934,685 + PGA1_c09100 0.18 +1.6 934,685 + PGA1_c09100 0.18 +2.1 934,693 - PGA1_c09100 0.18 +1.0 934,693 - PGA1_c09100 0.18 +1.9 934,693 - PGA1_c09100 0.18 +1.5 934,710 + PGA1_c09100 0.20 -0.2 934,719 - PGA1_c09100 0.21 -0.8 934,846 + PGA1_c09100 0.34 +1.7 934,854 - PGA1_c09100 0.35 +1.4 934,854 - PGA1_c09100 0.35 +2.0 934,903 + PGA1_c09100 0.40 +2.3 934,943 + PGA1_c09100 0.44 +0.7 935,068 - PGA1_c09100 0.57 +2.0 935,081 + PGA1_c09100 0.58 +1.5 935,089 - PGA1_c09100 0.59 +2.5 935,139 + PGA1_c09100 0.64 +0.9 935,147 - PGA1_c09100 0.65 +0.7 935,147 - PGA1_c09100 0.65 +2.4 935,172 + PGA1_c09100 0.67 +2.4 935,177 + PGA1_c09100 0.68 +2.1 935,217 - PGA1_c09100 0.72 +1.2 935,228 - PGA1_c09100 0.73 +1.5 935,276 - PGA1_c09100 0.78 +1.4
Or see this region's nucleotide sequence