Experiment: m.b. Fusidic 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c04930 and PGA1_c04940 are separated by 112 nucleotides PGA1_c04940 and PGA1_c04950 are separated by 296 nucleotides PGA1_c04950 and PGA1_c04960 are separated by 154 nucleotides
PGA1_c04930: PGA1_c04930 - tryptophan synthase alpha chain, at 484,569 to 485,360
_c04930
PGA1_c04940: PGA1_c04940 - Lipid A 3-O-deacylase (PagL)., at 485,473 to 485,985
_c04940
PGA1_c04950: PGA1_c04950 - GTP-dependent nucleic acid-binding protein EngD, at 486,282 to 487,379
_c04950
PGA1_c04960: PGA1_c04960 - putative non-specific ribonucleoside hydrolase, at 487,534 to 488,454
_c04960
Position (kb) 486
487
488 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 485.363 kb on - strand at 485.392 kb on - strand at 485.412 kb on + strand at 485.420 kb on - strand at 485.420 kb on - strand at 485.426 kb on + strand at 485.453 kb on + strand at 485.517 kb on + strand at 485.522 kb on + strand at 485.530 kb on - strand, within PGA1_c04940 at 485.548 kb on + strand, within PGA1_c04940 at 485.557 kb on + strand, within PGA1_c04940 at 485.557 kb on + strand, within PGA1_c04940 at 485.557 kb on + strand, within PGA1_c04940 at 485.557 kb on + strand, within PGA1_c04940 at 485.557 kb on + strand, within PGA1_c04940 at 485.562 kb on + strand, within PGA1_c04940 at 485.562 kb on + strand, within PGA1_c04940 at 485.565 kb on - strand, within PGA1_c04940 at 485.565 kb on - strand, within PGA1_c04940 at 485.593 kb on + strand, within PGA1_c04940 at 485.604 kb on + strand, within PGA1_c04940 at 485.649 kb on + strand, within PGA1_c04940 at 485.676 kb on + strand, within PGA1_c04940 at 485.684 kb on - strand, within PGA1_c04940 at 485.740 kb on + strand, within PGA1_c04940 at 485.746 kb on - strand, within PGA1_c04940 at 485.766 kb on - strand, within PGA1_c04940 at 485.766 kb on - strand, within PGA1_c04940 at 485.853 kb on + strand, within PGA1_c04940 at 485.853 kb on + strand, within PGA1_c04940 at 485.904 kb on - strand, within PGA1_c04940 at 485.943 kb on - strand at 485.947 kb on + strand at 485.947 kb on + strand at 485.947 kb on + strand at 485.947 kb on + strand at 485.947 kb on + strand at 485.955 kb on - strand at 485.955 kb on - strand at 485.955 kb on - strand at 485.964 kb on + strand at 485.992 kb on + strand at 485.992 kb on + strand at 485.999 kb on - strand at 486.000 kb on - strand at 486.073 kb on - strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.076 kb on + strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.084 kb on - strand at 486.140 kb on - strand at 486.173 kb on + strand at 486.190 kb on + strand at 486.202 kb on + strand at 486.212 kb on - strand at 486.216 kb on - strand at 486.234 kb on + strand at 486.244 kb on + strand at 486.255 kb on + strand at 486.273 kb on - strand at 486.520 kb on - strand, within PGA1_c04950 at 486.720 kb on + strand, within PGA1_c04950 at 486.969 kb on + strand, within PGA1_c04950 at 487.070 kb on + strand, within PGA1_c04950 at 487.381 kb on - strand at 487.383 kb on + strand at 487.454 kb on - strand at 487.472 kb on + strand at 487.480 kb on - strand at 487.509 kb on + strand at 487.509 kb on + strand at 487.517 kb on - strand at 487.566 kb on - strand at 487.573 kb on - strand at 487.610 kb on + strand at 487.653 kb on + strand, within PGA1_c04960 at 487.699 kb on + strand, within PGA1_c04960 at 487.700 kb on + strand, within PGA1_c04960 at 487.707 kb on - strand, within PGA1_c04960 at 487.708 kb on - strand, within PGA1_c04960 at 487.735 kb on + strand, within PGA1_c04960 at 487.759 kb on + strand, within PGA1_c04960 at 487.778 kb on + strand, within PGA1_c04960 at 487.820 kb on - strand, within PGA1_c04960 at 487.828 kb on - strand, within PGA1_c04960 at 487.832 kb on - strand, within PGA1_c04960 at 487.923 kb on - strand, within PGA1_c04960 at 487.935 kb on + strand, within PGA1_c04960 at 487.945 kb on + strand, within PGA1_c04960 at 487.959 kb on + strand, within PGA1_c04960 at 487.980 kb on - strand, within PGA1_c04960 at 487.988 kb on + strand, within PGA1_c04960 at 487.988 kb on + strand, within PGA1_c04960 at 487.988 kb on + strand, within PGA1_c04960 at 487.996 kb on - strand, within PGA1_c04960 at 487.996 kb on - strand, within PGA1_c04960 at 487.996 kb on - strand, within PGA1_c04960 at 487.996 kb on - strand, within PGA1_c04960 at 488.083 kb on - strand, within PGA1_c04960 at 488.096 kb on + strand, within PGA1_c04960 at 488.096 kb on + strand, within PGA1_c04960 at 488.096 kb on + strand, within PGA1_c04960 at 488.096 kb on + strand, within PGA1_c04960 at 488.096 kb on + strand, within PGA1_c04960 at 488.096 kb on + strand, within PGA1_c04960 at 488.104 kb on - strand, within PGA1_c04960 at 488.104 kb on - strand, within PGA1_c04960 at 488.115 kb on - strand, within PGA1_c04960 at 488.126 kb on - strand, within PGA1_c04960 at 488.146 kb on + strand, within PGA1_c04960 at 488.159 kb on + strand, within PGA1_c04960 at 488.164 kb on + strand, within PGA1_c04960 at 488.167 kb on - strand, within PGA1_c04960 at 488.177 kb on - strand, within PGA1_c04960 at 488.284 kb on + strand, within PGA1_c04960 at 488.286 kb on + strand, within PGA1_c04960 at 488.329 kb on + strand, within PGA1_c04960 at 488.332 kb on - strand, within PGA1_c04960 at 488.337 kb on - strand, within PGA1_c04960 at 488.337 kb on - strand, within PGA1_c04960 at 488.366 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Fusidic 0.0002 mg/ml remove 485,363 - +0.4 485,392 - +1.8 485,412 + +0.4 485,420 - -0.5 485,420 - +1.7 485,426 + -0.5 485,453 + -0.8 485,517 + +0.5 485,522 + +0.2 485,530 - PGA1_c04940 0.11 +1.1 485,548 + PGA1_c04940 0.15 +1.8 485,557 + PGA1_c04940 0.16 -0.3 485,557 + PGA1_c04940 0.16 -0.3 485,557 + PGA1_c04940 0.16 -0.7 485,557 + PGA1_c04940 0.16 +0.9 485,557 + PGA1_c04940 0.16 +0.7 485,562 + PGA1_c04940 0.17 -0.3 485,562 + PGA1_c04940 0.17 -2.1 485,565 - PGA1_c04940 0.18 +0.5 485,565 - PGA1_c04940 0.18 -0.5 485,593 + PGA1_c04940 0.23 -0.1 485,604 + PGA1_c04940 0.26 -0.1 485,649 + PGA1_c04940 0.34 +0.3 485,676 + PGA1_c04940 0.40 +0.7 485,684 - PGA1_c04940 0.41 -0.7 485,740 + PGA1_c04940 0.52 +1.2 485,746 - PGA1_c04940 0.53 +0.4 485,766 - PGA1_c04940 0.57 +0.7 485,766 - PGA1_c04940 0.57 +1.7 485,853 + PGA1_c04940 0.74 +0.2 485,853 + PGA1_c04940 0.74 -0.5 485,904 - PGA1_c04940 0.84 +1.1 485,943 - +0.5 485,947 + +0.1 485,947 + +0.2 485,947 + +0.3 485,947 + -0.8 485,947 + +0.4 485,955 - +0.7 485,955 - +0.7 485,955 - +0.8 485,964 + +0.5 485,992 + +0.4 485,992 + +1.7 485,999 - +1.2 486,000 - -0.9 486,073 - +0.1 486,076 + +0.1 486,076 + +0.0 486,076 + +1.2 486,076 + -1.3 486,076 + +1.1 486,076 + -0.4 486,076 + +0.2 486,076 + +0.3 486,076 + +0.6 486,076 + +0.1 486,076 + -0.5 486,076 + -0.2 486,076 + +0.4 486,076 + +0.9 486,076 + +0.5 486,076 + +2.0 486,076 + +0.2 486,076 + +0.9 486,076 + -0.3 486,076 + +0.8 486,076 + +1.0 486,076 + -0.5 486,076 + -1.3 486,076 + +1.9 486,076 + -0.3 486,076 + -0.3 486,076 + -0.2 486,076 + +1.2 486,076 + +0.2 486,084 - -0.0 486,084 - +0.4 486,084 - +0.3 486,084 - -0.1 486,084 - +0.1 486,084 - +0.5 486,084 - -0.7 486,084 - -0.1 486,084 - +0.1 486,084 - +1.8 486,084 - -0.3 486,084 - -0.6 486,084 - -0.7 486,084 - -0.9 486,084 - -0.5 486,084 - -0.5 486,084 - -1.7 486,084 - -0.5 486,084 - -0.2 486,084 - +0.7 486,084 - +0.3 486,084 - +1.1 486,084 - +0.2 486,084 - +3.2 486,084 - -0.1 486,084 - +0.2 486,084 - -0.6 486,084 - +1.4 486,084 - +1.0 486,084 - -1.7 486,084 - -0.1 486,084 - +0.4 486,084 - -0.1 486,084 - -0.3 486,084 - -0.1 486,084 - -1.2 486,084 - +0.3 486,140 - +0.3 486,173 + -0.0 486,190 + +0.1 486,202 + +0.3 486,212 - -0.3 486,216 - +0.2 486,234 + +0.7 486,244 + +0.4 486,255 + +0.2 486,273 - -0.5 486,520 - PGA1_c04950 0.22 -2.1 486,720 + PGA1_c04950 0.40 -2.8 486,969 + PGA1_c04950 0.63 +0.7 487,070 + PGA1_c04950 0.72 +0.7 487,381 - -0.1 487,383 + -0.5 487,454 - +1.0 487,472 + +1.1 487,480 - -0.1 487,509 + +1.4 487,509 + +0.2 487,517 - +2.5 487,566 - +2.7 487,573 - +1.1 487,610 + +1.8 487,653 + PGA1_c04960 0.13 +1.7 487,699 + PGA1_c04960 0.18 +2.2 487,700 + PGA1_c04960 0.18 +0.3 487,707 - PGA1_c04960 0.19 +0.1 487,708 - PGA1_c04960 0.19 +0.8 487,735 + PGA1_c04960 0.22 +2.5 487,759 + PGA1_c04960 0.24 +1.1 487,778 + PGA1_c04960 0.26 +1.0 487,820 - PGA1_c04960 0.31 +2.5 487,828 - PGA1_c04960 0.32 +2.8 487,832 - PGA1_c04960 0.32 +2.0 487,923 - PGA1_c04960 0.42 +1.8 487,935 + PGA1_c04960 0.44 +2.5 487,945 + PGA1_c04960 0.45 +1.8 487,959 + PGA1_c04960 0.46 +0.9 487,980 - PGA1_c04960 0.48 +2.4 487,988 + PGA1_c04960 0.49 +4.4 487,988 + PGA1_c04960 0.49 +1.5 487,988 + PGA1_c04960 0.49 +1.5 487,996 - PGA1_c04960 0.50 +3.0 487,996 - PGA1_c04960 0.50 +1.9 487,996 - PGA1_c04960 0.50 +1.3 487,996 - PGA1_c04960 0.50 +1.3 488,083 - PGA1_c04960 0.60 +2.5 488,096 + PGA1_c04960 0.61 +1.8 488,096 + PGA1_c04960 0.61 +1.7 488,096 + PGA1_c04960 0.61 +2.7 488,096 + PGA1_c04960 0.61 +1.9 488,096 + PGA1_c04960 0.61 +2.0 488,096 + PGA1_c04960 0.61 +1.2 488,104 - PGA1_c04960 0.62 +0.7 488,104 - PGA1_c04960 0.62 +1.2 488,115 - PGA1_c04960 0.63 +2.6 488,126 - PGA1_c04960 0.64 +1.4 488,146 + PGA1_c04960 0.66 +2.2 488,159 + PGA1_c04960 0.68 +1.7 488,164 + PGA1_c04960 0.68 +0.2 488,167 - PGA1_c04960 0.69 +2.1 488,177 - PGA1_c04960 0.70 +0.3 488,284 + PGA1_c04960 0.81 +3.1 488,286 + PGA1_c04960 0.82 +1.4 488,329 + PGA1_c04960 0.86 +1.7 488,332 - PGA1_c04960 0.87 +2.0 488,337 - PGA1_c04960 0.87 +1.9 488,337 - PGA1_c04960 0.87 +1.6 488,366 + +1.8
Or see this region's nucleotide sequence