Experiment: m.b. Fusidic 0.0002 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c04830 and PGA1_c04840 are separated by 344 nucleotides PGA1_c04840 and PGA1_c04850 overlap by 1 nucleotides PGA1_c04850 and PGA1_c04860 are separated by 7 nucleotides PGA1_c04860 and PGA1_c04870 are separated by 127 nucleotides
PGA1_c04830: PGA1_c04830 - 30S ribosomal protein S1, at 475,145 to 476,824
_c04830
PGA1_c04840: PGA1_c04840 - integration host factor subunit beta, at 477,169 to 477,453
_c04840
PGA1_c04850: PGA1_c04850 - Protein of unknown function (DUF1049)., at 477,453 to 477,803
_c04850
PGA1_c04860: PGA1_c04860 - N-(5'-phosphoribosyl)anthranilate isomerase TrpF, at 477,811 to 478,464
_c04860
PGA1_c04870: PGA1_c04870 - tryptophan synthase beta chain, at 478,592 to 479,845
_c04870
Position (kb)
477
478 Strain fitness (log2 ratio)
-2
-1
0
1 at 476.899 kb on + strand at 476.992 kb on + strand at 477.039 kb on - strand at 477.070 kb on - strand at 477.075 kb on + strand at 477.080 kb on + strand at 477.083 kb on - strand at 477.083 kb on - strand at 477.145 kb on + strand at 477.475 kb on + strand at 477.545 kb on + strand, within PGA1_c04850 at 477.749 kb on + strand, within PGA1_c04850 at 477.767 kb on + strand, within PGA1_c04850 at 477.783 kb on + strand at 477.808 kb on + strand at 478.460 kb on + strand at 478.460 kb on + strand at 478.501 kb on - strand at 478.559 kb on - strand at 478.579 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. Fusidic 0.0002 mg/ml remove 476,899 + +1.4 476,992 + +1.6 477,039 - +0.6 477,070 - +0.7 477,075 + +0.4 477,080 + -0.9 477,083 - -0.9 477,083 - +0.6 477,145 + +1.2 477,475 + -1.9 477,545 + PGA1_c04850 0.26 -1.1 477,749 + PGA1_c04850 0.84 -1.3 477,767 + PGA1_c04850 0.89 -0.2 477,783 + -0.7 477,808 + -0.3 478,460 + -0.9 478,460 + +0.1 478,501 - +0.1 478,559 - +0.8 478,579 + +1.5
Or see this region's nucleotide sequence